Abstract: An in vitro digestion device, adopting continuous dialysis method, was devised to imitate the nutrients digestion and absorption in crop, gizzard and proventriculus and small intestine of broiler. The suitable parameters, such as buffer flow rate, dialysis tube molecular weight cutoff, feed sample particle size and weight were studied too. Crude protein (CP) and starch digestibility rise with buffer flow rate. When buffer flow rate is 2.0ml/min both CP and starch digestibility is higher than when buffer flow rate is 1.0 ml/min and 1.5 ml/min respectively (P<0.01), but lower than 2.5ml/min (P<0.05). CP and starch digestibility were improved by increasing dialysis tube molecular weight cutoff from 12000 to 14000 Da and decreasing feed sample particle size from 40 Mu to 60 Mu. Feed sample weight also has effects on CP and starch digestibility in vitro. When1.5g feed sample digested in vitro CP and starch digestibility was lower than 0.5g and 1g (P<0.01), but there is no significant difference between 0.5g and 1g. In conclusion, the suitable buffer flow rate, size of dialysis tube molecular weight cutoff, particle size and weight of feed sample of this in vitro digestion device for broiler were 2.0ml/min,12000～14000Da, 60mu and 1g respectively. The device shows a good stability and replication. Both of CP and starch digestibility coefficient of variation in vitro are below 5%.