Abstract: Effect of three different soil types（gray damp soil，rice soil，Shajiang black soil） on the dynamic changes of starch accumulation and activities of five enzymes AGPP, UGPP, SSS, GBSS and SBE involved in starch synthesis was studied during grain filling of strong gluten wheat ZM 9023 on the condition of pond culture. Starch and components content was measured according to the method of double-wave length. AGPP and UGPP activities were tested according to the method described by Douglas et al，SSS、GBSS and SBE activities were tested according to the method described by Nakamura et al .The results indicated that the amylopectin synthesis was synchronous with as the amylose synthesis，and the amylopectin was synthetised faster than the amylose during the medium to later grain filling. The accumulating rates of amylopectin and starch in the kernel of gray damp soil were higher than that of other two soil types.The changes of five enzymes activities all came to be a single-peak curve, the peaks of AGPP, UGPP, SSS and SBE appeared in 18d after anthesis，but the peak of GBSS appeared in 24d after anthesis. The peaks of AGPP、SSS and SBE showed that “gray damp soil＞rice soil＞Shajiang black soil”, the peak of UGPP showed that “gray damp soil＞Shajiang black soil＞rice soil”，but the peak of GBSS indicated that “rice soil＞gray damp soil＞Shajiang black soil”. It stated that the gray damp soil and rice soil could help strong gluten wheat to synthetise starch. Correlation analysis showed that the activities of AGPP, UGPP, SSS, SBE in the kernel might be closely correlated with the amylopectin synthesis of strong gluten wheat in the gray damp soil, rice soil and Shajiang black soil.