Abstract: Polymerase chain reaction (PCR) conditions suitable for discriminating mungbean bruchid resistance genotypes were determined. Sixteen accessions were examined with 56 random primers which produced different PCR bands among them. These mungbean accessions can be classified into 4 groups, i. e., the wild-type resistance group (TC1966), the cultivar resistance group (V2709), the progeny resistance group(VC3890A2/TC1966-23) and the susceptible varieties with their progenies group. Mungbean cultivars with bruchid resistance or susceptibility and their crosses F2 of bruchid resistance wild-type with susceptible cultivar were used as materials in this experiment. By using BSA method, two codominant PCR markers were identified through the resistant (susceptible) bruchid cultivars bulks and the resistant (susceptible) bruchid bulks of a F2 population. There was a 1.03 kb band in the susceptible individuals and a 1.79 kb or two 1.79 kb/1.03 kb bands in the resistant individuals. It is sreculated that the markers are closely linked with TC1966 bruchid resistance/ susceptible elleles, and they can be applied in bruchid resistance identification of the mungbean and markers-assisted selection in bruchid resistance breeding of mungbean.

Key words: Mungbean [Vigna radiata(L.) Wilclzek], Bruchid resistance gene, PCR markers