Scientia Agricultura Sinica ›› 2025, Vol. 58 ›› Issue (15): 3036-3050.doi: 10.3864/j.issn.0578-1752.2025.15.008

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Effect of Applying Selenium Fertilizer to Soil on Selenium Enrichment of Winter Wheat in the Different Wheat-Producing Areas of China

LI HaiFeng1(), LI WenHu1, LI YuKe1, WANG ZhaoHui1,2, LIU JinShan1,2,*()   

  1. 1 College of Natural Resources and Environment, Northwest A&F University/Key Laboratory of Plant Nutrition and Agro- Environment in Northwest China, Ministry of Agriculture and Rural Affairs, Yangling 712100, Shaanxi
    2 State Key Laboratory of Crop Stress Biology Resistance and High-Efficiency Production, Yangling 712100, Shaanxi
  • Received:2024-09-27 Accepted:2024-11-21 Online:2025-08-01 Published:2025-07-30
  • Contact: LIU JinShan

Abstract:

【Objective】 Based on varying climate, soil, tillage, and field management conditions in different wheat-producing regions of China, this study aimed to investigate the impacts of soil-applied selenium (Se) fertilizer on Se enrichment in wheat and the available Se content in soil. Furthermore, the regional factors contributing to differences in the enhancement of Se content in wheat grain across these diverse areas were analyzed. These findings would provide a foundation for the efficient utilization of Se fertilizer and the scientific advancement of biofortified Se in wheat grain. 【Method】 The experiment was conducted from 2022 to 2024 in four representative wheat-producing regions of China, including Yongshou County of Shaanxi Province, Baixiang County of Hebei Province, Zitong County of Sichuan Province, and Shucheng County of Anhui Province. Five different levels of selenite fertilizer were applied: 0 (Se0), 100 (Se100), 200 (Se200), 300 (Se300), and 400 (Se400) g·hm-2. At the flowering and maturity stages of wheat, wheat plant and soil samples in each plot were collected. The wheat biomass, grain yield, Se content of different wheat organs, Se uptake and soil available Se content in different wheat-producing regions were determined and analyzed. 【Result】 Soil-applied Se fertilizer had no significant effect on wheat yield and aboveground biomass. Selenium content and accumulation in wheat organs were significantly increased with the increase of Se application rate. There was a linear positive correlation between Se content and Se application rate, and the selenium enrichment effect was in the order of Baixiang of Hebei Province > Yongshou of Shaanxi Province > Shucheng of Anhui Province > Zitong of Sichuan Province, and the Se content of grain was increased by 1.03, 0.57, 0.35, and 0.33 μg·kg-1, respectively with each application of 1 g·hm-2 selenium. Under the same Se application level, TFspike/stem and leaf value at flowering stage was higher than TFstem and leaf/root value, and TFgrain/stem and leaf value at maturity stage was increased with the increase of Se application rate, indicating that selenite was not easily transferred from root to stem and leaf, but easily transferred from stem and leaf to ear. The soil available Se content was significantly increased with the increase of Se application rate and the increasing effect was in the order of Yongshou of Shaanxi Province > Baixiang of Hebei Province > Shucheng of Anhui Province > Zitong of Sichuan Province, and the soil available Se content at the four sites was increased by 1.141, 0.077, 0.008, and 0.008 μg·kg-1, respectively with a rate of 1 g·hm-2 selenium application. The Se fertilizer application rate to meet the enrichment of grain selenium (150 μg·kg-1) in Yongshou of Shaanxi Province, Baixiang of Hebei Province, Zitong of Sichuan Province and Shucheng of Anhui Province were 232, 0, 376, and 354 g·hm-2, respectively. 【Conclusion】 In the wheat fields with low soil Se content, the application of Se fertilizer could lead to the production of Se-enriched wheat grain and an increase in the soil Se content in various wheat-producing regions of China. However, in alkaline soils, the enrichment of Se in wheat grain was more effective and the enhancement of soil available Se content was greater. The input rate of Se fertilizer for selenium enrichment requirement of wheat (>150 μg·kg-1) was lower in the alkaline soil than that in the acid soils.

Key words: main wheat-producing area, Se fertilization, grain Se content, soil Se content, Se biofortification

Table 1

Soil basic physicochemical properties of 0-20 cm layer at each field trial site in 2022"

地点
Site
pH 有机质
OM
(g·kg-1)
全氮
Total N
(g·kg-1)
硝态氮
NO3--N
(mg·kg-1)
铵态氮
NH4+-N
(mg·kg-1)
有效磷
Olsen-P
(mg·kg-1)
速效钾
Available K
(mg·kg-1)
总硒
Total Se
(mg·kg-1)
有效硒
Available Se
(µg·kg-1)
陕西永寿 Yongshou, Shaanxi 8.38 13.43 0.92 12.10 2.85 13.87 143.25 0.20 7.11
河北柏乡 Baixiang, Hebei 8.35 16.58 1.11 10.97 3.51 12.43 99.75 0.34 17.34
四川梓潼 Zitong, Sichuan 5.94 24.20 1.59 2.38 3.83 16.63 72.00 0.17 1.20
安徽舒城 Shucheng, Anhui 5.38 23.64 1.61 5.52 7.81 38.47 123.75 0.25 0.97

Table 2

Shoot biomass and grain yield of wheat under different Se application treatments"

年份
Year
地点
Site
产量 Yield (t·hm-2) 地上部生物量 Shoot biomass (t·hm-2)
Se0 Se100 Se200 Se300 Se400 Se0 Se100 Se200 Se300 Se400
2022-2023
陕西永寿 Yongshou, Shaanxi 6.52b 6.13b 6.20b 7.20a 6.33b 15.05b 14.41b 14.44b 16.72a 15.19b
河北柏乡 Baixiang, Hebei 8.49a 8.62a 8.58a 8.91a 8.64a 16.99a 17.42a 17.53a 18.63a 17.76a
四川梓潼 Zitong, Sichuan 7.05a 7.70a 7.43a 7.52a 7.61a 13.59a 14.79a 14.21a 14.54a 14.79a
安徽舒城 Shucheng, Anhui 5.91a 6.42a 6.21a 6.25a 6.14a 11.69a 12.21a 11.89a 12.04a 11.69a
2023-2024
陕西永寿 Yongshou, Shaanxi 7.75a 7.50a 7.73a 8.16a 8.04a 15.31a 15.00a 15.13a 15.78a 15.80a
河北柏乡 Baixiang, Hebei 9.09a 8.95a 9.33a 9.21a 8.74a 16.87a 16.54a 17.09a 17.11a 16.11a
四川梓潼 Zitong, Sichuan 7.98a 8.00a 8.33a 8.36a 8.20a 15.07a 14.70a 15.50a 15.31a 15.12a
安徽舒城 Shucheng, Anhui 4.94a 4.66a 5.55a 4.97a 5.26a 8.63a 8.03a 9.76a 8.74a 9.31a
两年均值
Two-year
average
陕西永寿 Yongshou, Shaanxi 7.14ab 6.92b 6.97ab 7.68a 7.19ab 15.18b 14.71b 14.79b 16.25a 15.49ab
河北柏乡 Baixiang, Hebei 8.79a 8.79a 8.95a 9.06a 8.69a 16.93a 16.98a 17.31a 17.87a 16.94a
四川梓潼 Zitong, Sichuan 7.52a 7.85a 7.88a 7.94a 7.91a 14.33a 14.75a 14.86a 14.92a 14.95a
安徽舒城 Shucheng, Anhui 5.43a 5.54a 5.88a 5.61a 5.70a 10.16a 10.12a 10.82a 10.39a 10.46a

Fig. 1

Se content in wheat organs under different Se application treatments Different lowercase letters indicate significant differences (P<0.05) in Se content among the five treatments in the same organs of wheat"

Fig. 2

Response of Se content in wheat grain to Se application rates"

Table 3

Se application rate for meeting the requirement of Se enrichment in wheat"

地点
Site
施硒量 Se application rate (g·hm-2)
2022-2023 2023-2024 平均 Average
陕西永寿 Yongshou, Shaanxi 183 281 232
河北柏乡 Baixiang, Hebei
四川梓潼 Zitong, Sichuan 370 382 376
安徽舒城 Shucheng, Anhui 353 356 354

Fig. 3

Se accumulation in wheat organs under different Se application treatments Different lowercase letters indicate significant differences (P<0.05) in Se accumulation among the five treatments in the same organs of wheat"

Table 4

The value of translocation factor (TF) under different selenium application treatments in 2024"

地点
Site
时期
Stage
TFSe 处理 Treatment
Se0 Se100 Se200 Se300 Se400
陕西永寿
Yongshou, Shaanxi
开花期 Flowering stage
TF穗/茎叶 0.30±0.09b 0.47±0.13ab 0.63±0.10a 0.71±0.40a 0.61±0.11ab
TF茎叶/根 0.08±0.04a 0.09±0.02a 0.11±0.03a 0.13±0.06a 0.12±0.02a
成熟期 Maturity stage TF籽粒/茎叶 0.41±0.21b 0.45±0.32b 1.10±0.27a 1.27±0.49a 1.23±0.18a
河北柏乡
Baixiang, Hebei
开花期 Flowering stage TF穗/茎叶 0.69±0.42a 0.58±0.21a 0.49±0.17a 0.51±0.19a 0.48±0.09a
TF茎叶/根 0.28±0.13b 0.28±0.09b 0.34±0.08ab 0.39±0.1ab 0.47±0.12a
成熟期 Maturity stage TF籽粒/茎叶 1.49±0.40a 1.60±0.56a 1.84±0.59a 1.94±0.70a 1.90±0.29a
四川梓潼
Zitong, Sichuan
开花期 Flowering stage TF穗/茎叶 0.48±0.33a 0.43±0.16a 0.45±0.08a 0.34±0.08a 0.43±0.06a
TF茎叶/根 0.12±0.05a 0.10±0.02a 0.10±0.02a 0.12±0.03a 0.09±0.02a
成熟期 Maturity stage TF籽粒/茎叶 0.53±0.16b 0.89±0.22ab 1.00±0.18ab 1.06±0.28ab 1.38±0.73a
安徽舒城
Shucheng, Anhui
开花期 Flowering stage TF穗/茎叶 0.41±0.15a 0.32±0.12a 0.30±0.08a 0.35±0.10a 0.38±0.14a
TF茎叶/根 0.12±0.02b 0.17±0.02ab 0.17±0.03ab 0.17±0.04ab 0.19±0.06a
成熟期 Maturity stage TF籽粒/茎叶 0.44±0.14c 0.60±0.22bc 0.74±0.12abc 0.91±0.19ab 1.02±0.31a

Table 5

Se biofortification index of wheat organs"

年份
Year
地点
Site
硒强化指数 Se biofortification index ((μg·kg-1)·(g·hm-2)-1)
茎叶 Straw 颖壳 Glume 籽粒 Grain 麸皮 Bran 面粉 Flour
2022-2023
陕西永寿 Yongshou, Shaanxi 0.31±0.08ab 0.22±0.03a 0.64±0.10b 0.80±0.12b 0.53±0.09b
河北柏乡 Baixiang, Hebei 0.33±0.02a 0.18±0.04ab 1.07±0.20a 1.38±0.13a 0.90±0.29a
四川梓潼 Zitong, Sichuan 0.23±0.03b 0.13±0.03b 0.31±0.04c 0.36±0.12c 0.29±0.04c
安徽舒城 Shucheng, Anhui 0.13±0.02c 0.15±0.02b 0.35±0.02c 0.60±0.03bc 0.17±0.04d
2023-2024
陕西永寿 Yongshou, Shaanxi 0.28±0.07b 0.05±0.01b 0.42±0.11b 0.58±0.17b 0.32±0.04b
河北柏乡 Baixiang, Hebei 0.57±0.07a 0.29±0.04a 1.14±0.18a 1.68±0.29a 0.85±0.12a
四川梓潼 Zitong, Sichuan 0.18±0.06b 0.09±0.01b 0.32±0.04c 0.47±0.09b 0.20±0.02bc
安徽舒城 Shucheng, Anhui 0.25±0.06b 0.06±0.01b 0.33±0.01c 0.57±0.08b 0.16±0.05c

Fig. 4

Soil available Se content in 0-20 cm soil layer after wheat harvest Different lowercase letters indicate significant differences (P<0.05) in Se content among the five treatments at the same experimental site"

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doi: 10.19586/j.2095-2341.2017.0095
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