电阻抗断层成像评价丰花月季抗寒性

doi:10.3864/j.issn.0578-1752.2017.07.013

Abstract

Abstract: 【Objective】 The objective of this experiment is to study whether the electrical impedance tomography (EIT) is feasible to assess the frost hardiness of floribunda roses (Rosa hybrida Hort. ‘Floribunda’), and with the view to provide a new reference method for rapid and non-destructive detection of ornamental plant for frost hardiness. 【Method】 Three perennial floribunda roses ‘Hongmao’, ‘Rouqingsishui’ and ‘Xianjing’ were used to measure EIT and electrical impedance spectroscopy (EIS) parameters of one-year-old stems under natural conditions of low temperature as well as artificial freezing, and the correlations of frost hardiness assessed by means of EIT and EIS parameters were compared with that of the traditional electrolyte leakage (EL) method. Meanwhile, the correlation analysis of four EIS parameters specific extracellular resistance (r_e), specific intracellular resistance (r_i), relaxation time (t) and distribution coefficient of t (y) of stems for three varieties of floribunda roses and EIT reconstructed values were studied.【Result】 The order of frost hardiness of the three floribunda roses varieties was: ‘Xianjing’＞‘Rouqingsishui’＞‘Hongmao’. Stems of the three varieties could achieve a clear EIT imaging at different temperatures and EIT reconstructed value showed a decreasing trend as temperature decreased. Frost hardiness calculated by EIT reconstructed value had a significant correlation (r=0.92) with that assessed by EL method, however, the results were lower than the EL method. EIS parameters changed during cold acclimation, on the whole, the extracellular resistance (r_e) rose, while the relaxation time (t) dropped. Under natural conditions of low temperature, r_e and EIT reconstructed values of ‘Hongmao’ and ‘Xianjing’ positively correlated (r＞0.72), and t and EIT reconstructed value of ‘Rouqingsishui’ and ‘Xianjing’ positively correlated (r＞0.63) as well, indicating that all of them can be used to characterize the state of cell and tissue.【Conclusion】 Under freezing temperature test, after frost damage EIT reconstructed values of stems in floribunda roses reduced significantly, and could be calculated with Logistic equation to get the frost hardiness, but the results were lower than those of EL method. Under natural conditions of low temperature, two EIS parameters extracellular resistance (r_e) and relaxation time (t) had correlations with EIT reconstructed values. After freezing test, the frost hardiness of the floribunda roses could be assessed by the EIT reconstructed values of stems.

Key words: floribunda roses, frost hardiness, electrical impedance tomography, electrical impedance spectroscopy parameters

JIAO MeiLing, GONG RuiJuan, QIAN Ji, ZHANG Gang. Frost Hardiness Assessment of Floribunda Roses by Means of Electrical Impedance Tomography[J].Scientia Agricultura Sinica, 2017, 50(7): 1302-1316.

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