2-甲基丁酸对犊牛小肠酶活及葡萄糖转运载体基因表达的影响

doi:10.3864/j.issn.0578-1752.2016.05.018

Abstract

Abstract: 【Objective】 There are obvious changes observed in the development of the gastrointestine and its function of absorption and metabolism in pre- and post-weaning dairy calves. Especially, the development of the gastrointestine and even its future production performance which are influenced by the feeding and management of calves during the pre-weaning period. 2-methyl butyric acid is a kind of branched-chain short volatile fatty acids, mainly from the degradation of branched chain amino acids in the rumen, can be used as a control agent of ruminant animal gastrointestinal development. The aim of this study was to reveal the 2-methyl butyric acid on development mechanism of the small intestine by observing the effects of 2-methyl butyrate supplementation on the small intestine digestive enzymes activities and expression of growth hormone receptor (GHR) and sodium-glucose cotransporter (SGLT1) mRNA of small intestinal mucosa in dairy calves. 【Method】Thirty-two Holstein male calves (15-day-old) with similar body weight (44.7±0.3) kg were chosen and divided into four groups randomly. Calves in the control were fed milk in pre-weaning and calf-concentrate and alfalfa hay in the post-weaning period. Calves in treatments were supplemented 2-methylbutyrate at 3, 6 and 9 g per calf per day, respectively. All calves were weaned at 45 days of age. At 30 and 90 days of age, four calves from each group were selected and slaughtered before the morning feeding. Digesta and mucosa of duodenal, proximal jejunal, distal jejunal and ileal were collected to determine the activities of lactase, amylase, trypsase and lipase, and mRNA expression of GHR and SGLT-1, respectively. 【Result】The results showed that lactase enzyme activity and mRNA expression of mucosal GHR and SGLT1 in every part of small intestine in post-weaning calves were lower than that in pre-weaning calves, while activities of amylase, lipase and trypsin in every part of the small intestine in post-weaning calves were higher than that of pre-weaning calves. Lactase activities of proximal jejunal, distal jejunal and ileal in pre-weaning calves supplemented with 2-methyl butyrate at 6 and 9 g·d^-1 were higher than that of the control group and the 3 g·d^-1 2-methyl butyrate group (P＜0.05). Lactase activities of duodenal and ileal in post-weaning calves in the group supplemented with 2-methyl butyrate at 6 and 9 g·d^-1 were higher than that of the control group and the 3 g·d^-1 2-methyl butyrate group (P＜0.05). Activities of trypsase and lipase in the duodenal, proximal jejunal, distal jejunal and ileal, and GHR mRNA expression of the small intestine in pre- and post-weaning calves supplemented with 2-methyl butyrate at 6 and 9 g·d^-1 were higher than that of the control group and the 3 g·d^-1 2-methyl butyrate group (P＜0.05). The SGLT-1 mRNA expression of the duodenal in pre- and post-weaning calves supplemented with 2-methyl butyrate at 6 and 9 g·d^-1 were higher than that of the control group (P＜0.05). The SGLT-1 mRNA expression of the proximal jejunal, distal jejunal and ileal in pre- and post-weaning calves supplemented with 2-methyl butyrate at 6 and 9 g·d^-1 were higher than that of the control and 3 g·d^-1 2-methyl butyrate group (P＜0.05).【Conclusion】Results indicated that digestive enzymes activities in the small intestinal digesta and mRNA expression of GHR and SGLT-1 in the small intestinal mucosa were improved by supplemental 2-methyl butyrate, and the optimum dose of 2-methyl butyric acid was 6 g·d^-1.

Key words: 2-methyl butyric acid, Holstein male calves, digestive enzymes activities, small intestinal mucosa, GHR mRNA, SGLT-1 mRNA

ZHANG Hang, LIU Qiang, WANG Cong, ZHANG Yan-li, PEI Cai-xia, WANG Yong-xin, GUO Gang, HUO Wen-jie, ZHANG Shuan-lin. Effects of 2-methylbutyrate on Digestive Enzymes Activities and Gene Expression of Glucose Cotransporter of Small Intestine in Calves[J].Scientia Agricultura Sinica, 2016, 49(5): 979-987.

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