Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (7): 1501-1508.doi: 10.3864/j.issn.0578-1752.2013.07.021

• RESEARCH NOTES • Previous Articles     Next Articles

Pheromonal Activity of Rectum Extracts from Chinese Citrus Fly (Bactrocera minax)

 XIAO  Wei, WU  Ke-Ming, GONG  Qing-Tao, ZHAO  Zhi-Mo, HE  Lin   

  1. 1.Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716
    2.Shandong Institute of Pomology, Taian 271000, Shandong
  • Received:2012-12-24 Online:2013-04-01 Published:2013-01-21

Abstract: 【Objective】The objective of this study is to make clear days that emerged adults of Chinese citrus fly (Bactrocera minax) need to reach sexual maturity, to obtain rectum extracts from adults fly and assay their pheromonal activity.【Method】Under a microscope, morphology and relative location of rectum in digestion system of adult flies were observed. Rectums from both males and females of 5-, 10- and 15-day-old adult flies were extracted with n-hexane. Male and female bodies with rectum excised were also extracted. EAG responses of male and female adults to rectum extracts and body extracts were recorded. Field attractiveness of rectum extracts from 15-day-old male and female adults were also investigated. 【Result】The rectum of B. minax was located at the distal part of digestion system, between hind gut and anus. The rectum was morphologically similar as that of other species in Bactrocera. EAG responses of male and female adults to heterosexual rectum extracts were the highest among all extracts. Meanwhile, male and female adults also showed relatively high EAG responses to homosexual rectum extracts. In field tests, 15-day-old female rectum extracts attracted significantly more adults than any one attractant of hydrolyzed protein lure, sugar solution with wine and vinegar mixture, and 15-day-old male rectum extracts. Meanwhile, even attractiveness of 15-day-old male rectum extracts was much lower than that of 15-day-old female rectum extracts, male rectum extracts still attracted a small number of adults. Sex ratio of adults attracted by 15-day-old female rectum extracts was 0.82﹕1, while any of other attractants attracted more females than males. 【Conclusion】About 15 days after emergency, Chinese citrus flies reaches sexual maturity. With similar morphology and location in digestion system as other species in Bactrocera, the rectum of B. minax also probably secrets and stores pheromonal substances in its rectal gland. Moreover, pheromones in female rectum are attractive to male and female adults in the field.

Key words: Chinese citrus fly (Bactrocera minax) , rectum extracts , pheromonal activity

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