Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (1): 136-145.doi: 10.3864/j.issn.0578-1752.2013.01.016

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Construction of Eukaryotic Expression Vector of Bovine fabp3 or fabp4 Gene and Expression of the Gene in Mouse Myoblasts

 WANG  Jie, AO  Xu-Dong, LI  Wen-Bin, BAI  Hai-Dong, YUE  Yong-Li, YU  Hai-Quan   

  1. The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Huhhot 010021
  • Received:2012-05-07 Online:2013-01-01 Published:2012-07-10

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Abstract: 【Objective】 The objective of the study is to analyze the intrinsic expression bovine fabp3 and fabp4 genes, and the dynamic changes of fabp3 and fabp4 gene expression after transfected the eukaryotic expression vector into mice myoblasts. 【Method】 Eukaryotic expression vector pDsHF3 for fabp3 gene, pDsHF4 for fabp4 gene were constructed and transfected into mice myoblasts by using liposome technique. The transfected mice myoblasts were induced differentiation into myotubes by 2% of pregnant mare serum after 72 h transfection. The changes of gene expression of myoblasts and myotubes were analyzed by real-time PCR. 【Result】 The results showed that, bovine fabp3 or fabp4 genes were expressed in transfected myoblasts, respectively. The intrinsic expression of mouse fabp3 or fabp4 gene was affected in myoblasts and in differentiated myotubes.【Conclusion】 Eukaryotic expression vectors pDsHF3 and pDsHF4 can highly express in proliferative myoblasts, and the expression of exogenous bovine fabp3 or fabp4 genes may affect intrinsic expression of mouse fabp3 and fabp4 genes in myoblasts and myotubes.

Key words: bovine fabp3 gene , bovine fabp4 gene , myoblasts , myotubes

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