高致病性PRRSV与PCV2共感染协同致病性研究

doi:10.3864/j.issn.0578-1752.2012.18.019

Abstract

Abstract: 【Objective】 The purpose is to study the synergetic pathogenic effect between highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) and porcine cirovirus type 2 (PCV2), and to clarify the relation of ordinal co-infection and synergetic pathogenicity. 【Method】 Thirty 35-day-old piglets were randomly selected and separated into 6 groups which designated PCV2/HP-PRRSV ordinal co-infection group, HP-PRRSV/PCV2 ordinal co-infection group, HP-PRRSV+PCV2 simultaneous co-infection group, HP-PRRSV infection group, PCV2 infection group and control group, respectively. Clinical symptom and temperature were recorded everyday post-inoculation, meanwhile, weighing body weight and collecting blood samples. Variations of CD3+CD4+CD8-, CD3+CD4-CD8+, γδT, NK, granulocyte and mononuclear cell were determined by flow cytometry. Two kinds of antibodies against HP-PRRSV and PCV2 were quantified by immunoperoxidase monolayer assay (IPMA). Concentrations of IFN-γ, TNF-α, IL-10, IL-2, GM-CSF in serum were determined by ELISA. Pathological changes were observed through making pathological section for different organs. 【Result】 All the findings suggest that the pathogenicity of HP-PRRSV/PCV2 ordinal co-infection is conspicuously more severe than other testing groups. Mortality of this group is 60% surprisingly. Virus load in body is the highest while antibodies level is the lowest. Variation of immunocyte subgroups and cytokines (especially TNF-α) is the most conspicuous. 【Conclusion】 There is a synergetic pathogenicity of co-infection of pigs with HP-PRRSV and PCV2. If HP-PRRSV infecting precedes PCV2, the morbility and mortality will be conspicuously enhanced. This study has provided a scientific proof to control the two viruses.

Key words: highly pathogenic porcine reproductive and respiratory syndrome virus, porcine cirovirus type 2, co-infection, synergetic pathogenicity

FAN Pei-Hu, WEI Yan-Wu, GUO Long-Jun, WU Hong-Li, HUANG Li-Ping, LIU Chang-Ming. Studies on Synergetic Pathogenicity of Co-infection with Highly Pathogenic PRRSV and PCV2[J].Scientia Agricultura Sinica, 2012, 45(18): 3859-3872.

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