Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (15): 3147-3153.doi: 10.3864/j.issn.0578-1752.2012.15.017

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Effect of Recipient Status and Embryo Transfer Methods on Production of Cloned Pigs

 WEI  Heng-Xi, LI  Qiu-Yan, GAO  Feng-Lei, LI  Yan, ZHANG  Shou-Quan, LI  Ning   

  1. 1.华南农业大学动物科学学院/广东省农业动物基因组学与分子育种重点实验室，广州 510642
    2.中国农业大学生物学院/农业生物技术国家重点实验室，北京100193
  • Received:2012-02-09 Online:2012-08-01 Published:2012-05-03

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Abstract: 【Objective】In order to establish an efficient embryo transfer technology and improve the production efficiency of cloned pigs, the present study was investigated different embryo transfer methods, gestational age and synchronization status of recipients on cloning pigs.【Method】Cloned embryos ware transferred with different methods to different status recipient, the delivery rates and the cloning efficiency were compared. The optimal synchronization time of recipient was determined by using co-transfer of different kinds of cloned embryos with similar developmental ability at different development stages.【Result】The method of transferring embryos through oviduct umbrella receive higher delivery rate and cloning efficiency (2.2% vs 0.4%, P＜0.01) than the method of oviduct puncture, and insemination after embryos transfer had a negative effect on cloning efficiency (0.6% vs 2.2%, P＜0.01). The litter size and cloning efficiency were higher when using sows as recipient than gilts ((5.5±0.7) vs (2.7±0.3), P＜0.05 and 3.0% vs 0.8%, P＜0.01 respectively). Higher cloning efficiency was found in the group of recipient estrous time posterior of 12-36 h to the embryo activation when compared to the meanwhile and prior of 12-24 h groups (2.0% vs 0.5% vs 0%, P＜0.05), and the optimal recipient synchronization time is the time of estrous posterior 24 h to embryo activation, and the cloning efficiency reached 3.0%.【Conclusion】An efficient embryos transfer technology was established in pig cloning by transferring embryos through oviduct umbrella and by using natural estrous sows beginning heat posterior 24 h to embryo activation as recipient.    

Key words: pig cloning, embryo transfer, recipient sows, cloning efficiency, somatic cell nuclear transfer

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