Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (4): 809-814.doi: 10.3864/j.issn.0578-1752.2012.04.023

• RESEARCH NOTES • Previous Articles     Next Articles

Comparison of Profiling Rice Volatiles by Means of Solid Phase Microextraction 

 SU  Yi-Juan, WANG  Rui-Long, YE  Mao, SONG  Yuan-Yuan, ZENG  Ren-Sen   

  1. 1.华南农业大学热带亚热带生态研究所/农业部生态农业重点开放实验室,广州 510642
  • Received:2011-04-07 Online:2012-02-15 Published:2011-11-30

Abstract: 【Objective】The objective of the study is to provide appropriate volatile collection methods of solid phase microextraction (SPME) for analyzing induced volatiles from plants through comparison of difference in rice volatile profiles collected by different fibres. 【Method】 Three SPME fibres including 100 µm polydimethylsiloxane (PDMS), 7 µm PDMS and 85 µm polyacrylate(PA) were used to extract volatiles emitted from rice plants that were treated with methyl jasmonate. Gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) were used to compare the difference in rice induced volatiles in terms of their amount, profile and composition of extracted volatiles. The difference between SPME method and dynamic headspace sampling (DHS) technique was investigated.  【Result】 Under the same condition, the maximum amount and variety of volatiles were found in rice volatiles extracted by 100 µm PDMS, the minimum amount and variety by 7 µm PDMS. The composition of volatiles obtained by SPME and DHS was similar, but profile of volatile compounds differed from each other. 【Conclusion】 Three fibres showed varied efficiency and selectivity in extracting volatile compounds. SPME fibres should be chosen appropriately according to the property of target compounds in plant volatiles. Both SPME and DHS have their own advantages and disadvantages in plant volatiles extraction and analysis.

Key words: rice volatiles, solid phase microextraction (SPME), dynamic headspace sampling (DHS)

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