Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (19): 4102-4109.doi: 10.3864/j.issn.0578-1752.2011.19.022

• VETERINARY SCIENCE • Previous Articles     Next Articles

Studies on Pharmacodynamics in vitro of the Florfenicol Dual Suspension Emulsion

 LI  Xiao-Wen, DONG  Wei, YANG  Kun, CHEN  Xiao-Jun, LIU  Zhao-Ying, SUN  Zhi-Liang   

  1. 1.湖南农业大学动物医学院
    2.湖南省兽药工程技术研究中心
    3.国家植物功能成分利用工程技术研究中心生物兽药分中心
  • Received:2011-05-23 Online:2011-10-01 Published:2011-08-23

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Abstract: 【Objective】 The in vitro pharmacodynamics characteristics of florfenicol dual suspension emulsion (DSEF) were studied in an experiment. 【Method】 The florfenicol injection (FI) was used as the control group and five kinds of bacteria were used for in vitro antibacterial test to determine the minimal inhibitory concentration (MIC), minimal bactericide concentration (MBC) and mutant selection window (MSW), respectively. The S.typhimurium was used for in vitro antibacterial test about post-antibiotic effect (PAE) and post-antibiotic sub-MIC effect (PASME), respectively. 【Result】 Compared with the ordinary injection, the MIC and MBC of DSEF for five kinds of bacteria showed no change obviously. But DSEF narrowed MSW obviously of five kinds of bacteria (P＜0.01) and extended the PAE and PASME significantly of S. typhimurium (P＜0.01). 【Conclusion】DSEF could reduce the bacterial resistance risk and extend the time of its after effect obviously, thereby improved the antibacterial effect.

Key words:

[1]Priebe S, Schwarz S. In Vitro activities of florfenicol against bovine and porcine respiratory tract pathogens, Antimicrob Agents Chemother, 2003, 47(8): 2703–2705.

[2]Park B K, Lima J H, Kima M S, Hwanga Y H, Yun H I. Pharmacokinetics of florfenicol and its metabolite, florfenicol amine, in dogs. Research in Veterinary Science, 2008, 84(1):85-89.

[3]曾振灵, 宋冶萍, 杨桂香, 叶启薇, 陈杖榴. 氟苯尼考与氯霉素对鸡大肠杆菌的疗效比较. 中国兽医科技, 2001, 8(31):25-27.

Zeng Z L, Song Y P, Yang G X, Ye Q W, Chen Z L. Efficacy compared about florfenicol and chloramphenicol for chicken E. coli. Chinese Journal of Veterinary Science and Technology, 2001, 8(31):25-27. (in Chinese)

[4]魏海涛, 方秋华, 曾振灵, 黄显会. 氟苯尼考药剂学研究进展. 中国兽药杂志, 2010, 44(7) : 44-46.

Wei H T, Fang Q H, Zeng Z L, Huang X H. Research progress on pharmaceutics of florfenicol. Chinese Journal of Veterinary Drug, 2010, 44(7) : 44-46. (in Chinese)

[5]孙志良, 李孝文, 董 伟, 陈小军， 刘 晗. 一种兽用抗菌油、水双混悬型注射用乳剂及其制备方法. CN102119923A, 2011-07-13.

Sun Z L, Li X W, Dong W, Chen X J, Liu H. The Dual Suspension Emulsion in oil and water about antibacterial drugs for veterinary use and its preparation methods. CN102119923A, 2011-07-13. (in Chinese)

[6]胡功政, 苑  丽, 刘智明, 王升运, 王付民. 氟苯尼考及其与多西环素联合的体外抗菌作用. 中国兽医学报, 2004, 24(4): 379-382.

Hu G Z, Yuan L, Liu Z M, Wang S Y, Wang F M. Antibacterial activity of florfenicol and its combinations with doxycycline in vitro. Chinese Journal of Veterinary Science, 2004, 24(4): 379-382. (in Chinese)

[7]苑 丽, 胡功政, 刘智明, 王升运, 梁 军, 杨玉荣. 氟苯尼考与多西环素联合对鸡大肠杆菌病的治疗效果. 河南农业大学学报, 2005, 39(1):93-97.

Yuan L, Hu G Z, Liu Z M, Wang S Y, Liang J, Yang Y R. Treament eficacy of florfenicol and its combinations against E.coli in chicken. Journal of Henan Agricultural University, 2005, 39(1):93-97. (in Chinese)

[8]廖昌容, 徐力文, 陈毕生. 氟苯尼考对六种海洋致病弧菌的体外抗菌活性研究. 水产养殖, 2005, 26(4): 1-4.

Liao C R, Xu L W, Chen B S. In vitro antibacterial activities of florfenicol against six marine pathogenic Vibrios. Journal of Aquaculture, 2005, 26(4):1-4.(in Chinese)

[9]李忠琴, 关瑞章, 汪黎虹, 骆曼娜, 刘宏伟. 氟苯尼考对鳗鲡常见致病菌体外抗菌后效应研究. 中兽医医药杂志, 2010, 4:40-42.

Li Z Q, Guan R Z, Wang L H, Luo M N, Liu H W. Post-antibiotic effect of florfenicol on pathogenic bacteria of eels in vitro. Journal of Traditional Chinese Veterinary Medicine, 2010, 4:40-42. (in Chinese)

[10]张 旭, 王大菊, 伍金娥. 氟苯尼考对禽多杀性巴氏杆菌的抗菌后效应. 华中农业大学学报, 2006, 25(4):408-410.

Zhang X, Wang D J, Wu J E. Post-antibiotic effect of florfenicol against pasteurella multocid(birds) in vitro. Journal of Huazhong Agricultural University,2006, 25(4):408-410. (in Chinese)

[11]张 旭, 薛克友, 王大菊, 赵芬琴, 谢欣梅. 氟苯尼考在鸡体内的药动学及其体内抗菌后效应研究. 中国兽药杂志, 2008, 42(12):39-42.

Zhang X, Xue K Y, Wang D J, Zhao F Q, Xie X M. Studies on pharmacokinetics of florfenicol in chicken and its post-antibiotic effects in vitro. Chinese Journal of Veterinary Drug, 2008, 42(12): 39-42. (in Chinese)

[12]张世新. 氟苯尼考对大肠杆菌K99体外抗菌后效应研究. 中国兽医杂志, 2006, 8(42):52-53.

Zhang S X. The in vitro post-antibiotic effects of the florfenicol for E. coli K99. Chinese Journal of Veterinary Medicine, 2006, 8(42):52-53. (in Chinese)

[13]吴小宁. 氟苯尼考脂质体的制备及药效学研究[D]. 杨凌: 西北农林科技大学硕士学位论文, 2005: 05.

Wu X N. Preparation and study on pharmacy of forfenicol- liposome[D]. Yangling: Northwest Sci-Tech University of Agriculture and Forestry, 2005: 05. (in Chinese)

[14]Zhao X, Drlicab K. Restricting the selection of antibiotic-resistant mutant bacteria: measurement and potential use of the mutant selection window. Journal of Infectious Diseases, 2002, 185(4): 561-565.

[15]Drlica K. The mutant selection window and antimicrobial resistance. Journal of Antimicrobial Chemotherapy, 2003, 52(1): 11-17.

[16]刘 洋, 徐 刚, 王 敏, 李洪涛, 李艳华. 酒石酸泰乐菌素联合阿莫西林钠缩小猪链球菌耐药突变选择窗的研究. 中国预防兽医学报, 2010, 32(1):65-67.

Li Y, Xu G, Wang M, Li H T, Li Y H. Narrowing mutant selection window of Streptococcus suis by combination use of tylosin tartrate and amoxicillin sodium in vitro. Chinese Journal of Preventive Veterinary Medicine, 2010, 32(1):65-67. (in Chinese)

[17]Peter A. James. Comparison of four methods for the determination of MIC and MBC of penicillin for viridans streptococci and the implications for penicillin tolerance. Journal of Antimicrobial Chemotherapy, 1990, 25(2): 209-216.

[18]张致平. 微生物药物学. 北京: 化学工业出版社, 2003: 388-394.

Zhang Z P. Microbial Pharmacology. Beijing: Chemical Industry Press, 2003: 392-394. (in Chinese)

[19]李国涛, 鲁猛厚. 氟喹诺酮类药物对伤寒沙门菌的防突变浓度测定. 中国传染病杂志, 2007, 25(5): 276-278.

Li G T, Lu M H. The determination of mutant prevention concentration for Salmonella bacteria about Fluoroquinolones drugs. Chinese Journal of Infectious Diseases, 2007, 25(5): 276-278. (in Chinese)

[20]Zinner S H, Lubenko I Y, Gilbert D, Simmons K, Zhao X, Drlica K, Firsov A A. Emergence of resistant Streptococcus pneumoniae in an in vitro dynamic model that simulates moxifloxacin concentrations inside and outside the mutant selection window: related changes in susceptibility, resistance frequency and bacterial killing. Journal of Antimicrobial Chemotherapy, 2003, 52(4):616-622.

[21]刘明涛, 孙恩华, 毕少杰, 于瑞丛, 李 玉. 利奈唑胺与万古霉素对金黄色葡萄球菌耐药突变选择的比较. 山东大学学报: 医学版, 2009, 47(8):42-45.

Liu M T, Sun E H, Bi S J, Yu R C, Li Y. Comparison of linezolid and vancomycin in preventing the drug resistant mutants for staphylococcus aureus. Journal of Shan Dong University: Health Sciences, 2009, 47(8): 42-45. (in Chinese)

[22]Hanberger H, Svensson E, Nilsson L E, Nilsson M. Pharmacodynamic effects of meropenem on gram-negative bacteria. European Journal of Clinical Microbiology & Infectious Diseases, 1995, 14: 383-390.

[23]王 慧, 刘 伟, 陈小军, 刘自逵, 孙志良. 头孢噻呋混悬剂体外抗菌活性及抗生素后效应研究. 中国农业科学, 2010, 43(7): 1493-1499.

Wang H, Liu W, Chen X J, Liu Z K, Sun Z L. The in vitro antibacterial activities and post-Antibiotic effects of the ceftiofur suspension. Scientia Agricultura Sinica 2010, 43(7):1493-1499. (in Chinese)

[24]王丽平, 江善祥, 史晓丽, 郭永刚, 陈绍峰. 氨苄西林与阿莫西林对金黄色葡萄球菌及大肠杆菌的体外抗菌后效应. 南京农业大学学报, 2004, 27(1):93-96.

Wang L P, Jiang S X, Shi X L, Guo Y G, Chen S F. Postantibiotic efect of ampicillin and amoxycillin on Staphylococcus aureus and Escherichia coli in vitro. Journal of Nanjing Agricultural University, 2004, 27(1):93-96. (in Chinese)

[25]徐叔云, 卞如濂, 陈 修．药理实验方法学. 第3版．北京:人民卫生出版社, 2002: 1656-1659.

Xu S Y, Bian Y L, Chen X. Pharmacology Experiment Methodology. The third edition. Beijing: People’s Medical Publishing House, 2002: 1656-1659. (in Chinese)

[26]王志强, 陈杖榴. 兽用氟喹诺酮类的抗菌后效应研究. 中国兽药杂志, 2001, 35(5):13-17.

Wang Z Q, Chen Z L. The post-antibiotie effects of fluoroqujnolones for veterinary use in vitro. Chinese Journal of Veterinary Drug, 2001, 35(5):13-17. (in Chinese)

[27]张 旭. 氟苯尼考抗菌后效应及在鸡体内药物动力学研究[D]. 武汉: 华中农业大学硕士学位论文, 2005: 5.

Zhang X. Studies on the post-antibiotic effects and pharmacokinetics of florfenicol in chicken[D]. Wuhan: Huazhong Agricultural University, 2005: 5. (in Chinese)

[28]雷连成, 陈 伟, 韩文瑜, 蒲 明. 氧氟沙星脂质体的制备及抑菌实验观察. 中国兽药杂志, 2002, 36(5):33-36.

Lei L C, Chen W, Han W Y, Fu M. The preparation of levofloxacin liposomes and its antibacterial experiment. Chinese Journal of Veterinary Drug, 2002, 36(5):33-36. (in Chinese)

[29]Song J H, Kim S K, Chang K W, Han S K, Yi H K, Jeon J G. In vitro inhibitory effects of Polygonum cuspidatum on bacterial viability and virulence factors of Streptococcus mutans and Streptococcus sobrinus. Archives of Oral Biology, 2006, 51(12): 1131-1140.

[30]Rotter M, Sattar S, Dhara S, Allegranzi B, Mathai E, Pittet D. Methods to evaluate the microbicidal activities of hand-rub and hand-wash agents. Journal of Hospital Infection, 2009, 73(3): 191-199.

[31]何 敏, 卢胜明, 李英伦. 氟苯尼考耐药性研究进展. 中国兽药杂志, 2005, 39(6):38-41.

He M, Lu S M, Li Y L. Development in the research of florfenicoi resistance in bacteria. Chinese Journal of Veterinary Drug, 2005, 39(6): 38-41. (in Chinese)

[32]崔俊昌, 刘又宁．抗菌药物防突变浓度及其临床意义.国外医学: 呼吸系统分册, 2003, 6(23):3l5-318.

Cui J C, Liu Y N. Antibacterial drugs mutant prevention concentration and their clinical significance. Section of Respiratory System Foreign Medical Sciences, 2003, 6(23):3l5-318. (in Chinese)

[33]李朝霞, 刘又宁. 细菌耐药突变选择窗理论与抗菌药物合理应用. 中国药学杂志, 2006, 4l(16):1201-1204.

Li Z X, Liu Y N. The theory of bacteria mutant selection window and the reasonable application of antimicrobial agents. Chinese Pharmaceutical Journal, 2006, 4l(16): 120l-1204.(in Chinese)

[34]Zhou J, Dong Y, Zhao X, Lee S, Amin A, Ramaswamy S, Domagala J, James M, Musser, Drlica K. Selection of antibiotic-resistant bacterial mutants: allelic diversity among fluoroquinolone-resistant mutations. Journal of Infectious Diseases, 2002, 182(2):517-525.
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