Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (18): 3903-3908.doi: 10.3864/j.issn.0578-1752.2011.18.022

• VETERINARY SCIENCE • Previous Articles     Next Articles

Forsythiaside A Induced IFN-α and Up-Regulated Correlation Factors of JAK-STAT Signaling Pathways in vitro

 LI  Hua-Wei, ZHANG  Yu-Lu, ZHANG  Zhong-Wen, XIA  Nan, GUO  Wei, WU  Guo-Juan   

  1. 1.北京农学院动物科学技术学院
  • Received:2010-11-09 Revised:2011-03-16 Online:2011-09-15 Published:2011-05-25

Abstract: 【Objective】 This study focused on the effect of forsythiaside A added in chick embryo kidney cells, the expression of IFN-αand the mRNA expression of correlation factors in JAK-STAT pathway. 【Methods】 In the study, three levels forsythiaside (100, 200, 400 μg•mL-1) were set, and real-time RT-PCR was used to detect the mRNA expression of IFN-α and JAK-STAT pathway related factors; western blotting was used to investigate protein expression of IFN-α. 【Result】 Compared with normal group, the expression of IFN-α increased significantly when high dose forsythiaside A was added (P<0.05). And the expression of STAT1, JAK1, IFNAR1, IFNAR2, IRF1, IRF7 was significantly increased either. 【Conclusion】Forsythiaside A induced the expression of IFN-α in chicken embryo kidney cells (CEKs), and can positively regulate the JAK-STAT signaling pathway significantly.

Key words: forsythosideA, CEKcell, IFN-α, JAK-STAT, real-timePCR, westernblotting

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