Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (18): 3886-3894.doi: 10.3864/j.issn.0578-1752.2011.18.020

• VETERINARY SCIENCE • Previous Articles     Next Articles

Genetic Variation of the ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus in East China from 2008 to 2010

 WANG  Xiao-Min, HE  Kong-Wang, ZHANG  Wen-Wen, CHEN  Wei, MAO  Ai-Hua, YU  Zheng-Yu, WEN  Li-Bin, NI  Yan-Xiu, ZHANG  Xue-Han, 吕Li-Xin , GUO  Rong-Li, ZHOU  Jun-Ming, LI  Bin   

  1. 1.江苏省农业科学院兽医研究所/农业部动物疫病诊断与免疫重点开放实验室/国家兽用生物制品工程技术研究中心
    2.南京农业大学动物医学院
  • Received:2011-04-14 Revised:2011-06-23 Online:2011-09-15 Published:2011-08-12
  • Contact: Xiaomin Wang E-mail:JAAS.WXM@Gmail.com

Abstract: 【Objective】 A total of 260 swine samples of dead or sick pigs obtained from Jiangsu, Anhui, Shanghai, Shandong, Zhejiang, Fujian and Jiangxi provinces of China during 2008-2010 were detected for PRRSV. And ORF5 genes of some isolates were amplified and sequenced for understanding the molecule epidemiology and the genetic evolution of porcine reproductive and respiratory syndrome virus (PRRSV) in East China.【Method】PRRSV was identified by RT-PCR from samples. Sequences of ORF5 of 36 PRRSV positive samples was amplified and analyzed with other 15 strains available on NCBI GenBank.【Result】 A total of 118 samples were positive and the positive rate was 45.4%. Sequence analysis showed that the 36 isolates of this study belong to the North American-type PRRSV strains and were closely related to the highly pathogenic PRRSV (HP-PRRSV) with 94.6-100% amino acid sequence identities. The sequence analysis combined with the phylogenetic analysis indicated that all these North American-type PRRSV strains in East China were further divided into five subgenotypes, subgenotype III showed colser identity with HP-PRRSV; almost all subgenotypes were found to be variable in the primary neutralizing epitope and subgenotypes III and IV had more glycosylation sites than others. Although these 36 isolates were collected from different provinces in East China, there were no obvious relations between the distribution of PRRSV and the region.【Conclusion】 The PRRSV infection was widespread and HP-PRRSV was the popular strain in East China during 2008-2010. However some different genetic characteristics appeared in the genomes, the genetic evolution was relatively stable. There exists a cross-cutting phenomenon on the genetic relationship of PRRSV isolates obtained from different provinces. Subgenotype IV and V only appeared in some provinces, but the distribution of PRRSV did not show apparent geographical characteristics.

Key words:

CLC Number: 

  • S855.3

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