Abstract: 【Objective】To analysis the genetic differentiation of the host- and geo-populations of Liriomyza sativae Blanchard.【Method】Theβ-tubulin gene of 5 host-populations and 6 geo-populations of Liriomyza sativae Blanchard were sequenced. The identity and variation of β-tubulin gene sequenced were analyzed by biosoftware DNAStar and MEGA and the phylogenetic trees were constructed. 【Result】The results obtained by the biosoftware DNAStar and MEGA respectively were similar. The differences of β-tubulin gene among the host- and geo-populations of L.sativae were very low, with 8 variable sites and no insertions and deletions and the identities were all above 98%.【Conclusion】The trends of differentiation of host- and geo-populations of L. sativae were consistent with the hobby to hosts and the geographical distribution, respectively.