Abstract: 【Objective】】To research the inhibition effects of compound Chinese medicine on the anti-avian influenza virus in vivo and in vitro, its pharmacokinetics and acute toxicity test , its effects on immune function of SPF chicken.【Method】the effects of direct inactivation,preventive and therapeutic of compound Chinese medicine on the anti-avian influenza virus were studied on chicken embryo and MDCK cell. Its pharmacokinetics was studied by drug residual accumulation. The acute toxicity test of compound Chinese medicine was studied by oral administration and intraperitoneal injection on Kunming mice. The effects of compound Chinese medicine on immune function of SPF chicken was studied by means of the indexes of immune organs in chickens,the contents of CD3、CD4、CD8 andγδTCR by FAC and antibody titer of avian influenza virus detected on HI method.【Result】The result showed that the compound medicine had a strong action of direct inactivation,preventive and therapeutic on the anti-avian influenza virus in vitro. Furthermore it inhibited avian influenza virus adsorbed in cell surface and entered into intracellular. It could prolong the mean death time of chicken and took effect on the chicken challenged the subtype H5N1 of highly avian influenza virus in vivo. The pharmacokinetics parameter showed that its elimination was slow. It had a long time in the mouse and it had long effect in the mouse. The study of acute toxic experiments on mouse was as following: After oral administration the compound medicine was safe. After intraperitoneal injection adminwastration, the LD50 was 425.6mg/kg.b.w.It enhanced cellular and humoral immune function and the indexes of immune organs in chickens.【Conclusion】The compound Chinese medicine is preventive and safe , prolong the mean death time of chicken and strengthen immunity of chickens.