Abstract: Abstract: 【Objective】Development of multiplex PCR for wheat quality traits is very important to improve the efficiency and reduce the cost in molecular marker assisted breeding. 【Method】 In the present study, according to genes controlling for pan bread and Chinese noodle quality, and the annealing temperatures of different markers, the molecular markers for important quality trait genes, i.e., high-molecular-weight glutenin subunit (HMW-GS) genes Ax2*, Bx14, Bx17 and Dx5, and low-molecular-weight glutenin subunit (LMW-GS) gene Glu-A3d, as well as the marker BDFL-BRD for Wx-B1, MAG269 for Wx-D1, ω-sec for 1BL/1RS translocation and PPO18 for polyphenol oxidase（PPO）activity, were selected and three combinations of molecular markers Ax2*/Bx17/Dx5, Bx14/Glu-A3d/ω-sec and BDFL-BRD/MAG269/PPO18 were tested. 【Result】Results indicated that the three multiplex PCRs, PCR-I (Ax2*/Bx17/Dx5), PCR-II (Bx14/Glu-A3d/ω-sec) and PCR-III (BDFL-BRD/MAG269/PPO18) can be used to identify the genes presented in wheat cultivars. A total of 141 wheat cultivars were evaluated with the multiplex PCRs, and the frequencies of HMW-GS Ax2*, Bx14, Bx17, Dx5 and LMW-GS Glu-A3d accounted for 4.3%, 7.1%, 1.4%, 17.7% and 27.7%, respectively. Frequencies of 1BL/1RS translocation and low polyphenol oxidase (PPO) activity genetypes were 44.0% and 51.8%, respectively. Wx-B1 null type accounted for 4.3%. Data obtained by SDS-PAGE for HMW-GS, LMW-GS and secalin for 80 genotypes correspond very well with those from the multiplex PCRs. 【Conclusion】This indicated that the multiplex PCRs developed can be steadily and efficiently used to detect the nine genes for the improvement of wheat quality in molecular marker assisted breeding.