Abstract: Chemical oxygen demand, weight change, waste water composition were assayed with CODMn filter and membrane separation, DNS, Comassie brilliant blue during bio-pulping, bio-retting and bio-saccharification process of herbaceous fibers. It was revealed that non-cellulose equivalent to about 10% of total raw material weight could be consumed in the fermentation stage, and another 15% of total raw material weight including 90% in the form of macromolecule or particle (The left 10% was soluble reducing sugar and protein) could be removed in the coming washing stage. So the pollution charge could be greatly reduced originally and most of the pollutant could be simply separated with physical method. The final waste water could be easily treated.

Key words: herbaceous fiber, bio-extraction, pollutant analysis