关键词: 甘蓝型油菜, 显性核不育, 波里马胞质不育, 遗传模式

Abstract: Abstract: 【Objective】The dominant genic and cytoplasmic male sterility (DGCMS) in Brassica napus might be a new way in breeding program and utilization of heterosis. The present study was designated to verify the genotype of dominant male sterility two-line and polcms three lines, identify the genetic pattern of dominant male sterility of Gd1AB and D3AB, construct dominant genic and cytoplasmic male sterility system. 【Method】Various types of testcrosses and backcrosses were made between dominant male sterility two-line and polcms three lines, and the fertility segregation was recorded respectively.in ChongQing and/or ShiZhu country in spring and summer season . The allelism between the Mf in Gd1AB or D3AB and Rfc in polcms restorers was analyzed. The testcrosses were made through crossing Gd1A or D3A with dominant restorers 05R or D1R, and the sterile plants of F2 with temporary maintainer test methodology were subjected to determination of the genetic pattern of dominant homozygous two-type line Gd1AB or D3AB. The sterile plants with polcms cytoplasm were selected to pollinated with C3B as recurrent parent, extensively screening in testcross progenies was made to find dominant nucleus + cytoplasm twin-ms lines. 【Result】The testcross populations containing only sterile individuals clearly indicated the multiple allele inheritance model for Gd1AB was confirmed, and then the fact that testcross lines segregated for feitility strongly supports D3AB was conditioned by two dominant interactive genes rather than by multiple alleles. The dominant hyterozyous genic and cytoplasmic male sterility line DGCMS—3A was successfully obtained by continuous recurrent selection cross toward higher quality trait with low erucic acid and low glucosinolate for two years. By temporary maintainer test methodology, it was possible to select dominant homozygous genic male sterility with polcms cytoplasm by recurrent selection through crossing Gd1B or D3B. 【Conclusion】The inhibitory gene Mf in the fertile plants from Gd1AB or D3AB was demonstrated to be norrallelic to the gene Rfc from polcms restores. The genotype of the sterile and fertile plants in Gd1AB are N(MsMsrfcrfc) and N(MfMsrfcrfc) respectively, while D3AB are of genotypes N(MsMsmfmfrfcrfc) and N(MfMsmfmfrfcrfc) respectively. Therefore, the dominant genic and cytoplasmic male sterility were separated into four kinds: the two dominant interactive hyterzygous genic and cytoplasmic male sterility S(Msmsmfmfrfcrfc)+S(msmsmfmfrfcrfc), the single multiple allelic hyterzygous genic and cytoplasmic male sterility S(Msmfrfcrfc)+S(mfmfrfcrfc), the two dominant interactive homozygous genic and cytoplasmic male sterility S(MsMsmfmfrfcrfc)+S(MsMsMfmfrfcrfc), the single multiple allelic homozygous genic and cytoplasmic male sterility S(MsMsrfcrfc)+S(MfMsrfcrfc).

Key words: Brassica napus L., Dominant genic male sterility, Polima cytoplasmic male sterility, Inheritance model