芽孢杆菌脂肽靶向减弱青枯雷尔氏菌致病力的生防作用

doi:10.3864/j.issn.0578-1752.2026.13.008

中国农业科学 ›› 2026, Vol. 59 ›› Issue (13): 2853-2866.doi: 10.3864/j.issn.0578-1752.2026.13.008

芽孢杆菌脂肽靶向减弱青枯雷尔氏菌致病力的生防作用

刘羽凡¹^,³(), 陈峥²(), 陈德局¹, 刘欣¹, 肖荣凤¹, 王阶平¹, 王璕³, 刘波¹, 何进³(), 陈梅春¹()

¹ 福建省农业科学院资源环境与土壤肥料研究所，福州 350003
² 福建省农业科学院植物保护研究所，福州 350003
³ 华中农业大学农业微生物资源发掘与利用全国重点实验室，武汉 430000

收稿日期:2026-02-25 接受日期:2026-05-01 出版日期:2026-07-01 发布日期:2026-07-01
通信作者:
何进，E-mail：hejin@mail.hzau.edu.cn
陈梅春，E-mail：cmczjw@163.com
联系方式: 刘羽凡，E-mail：liuyufan12256@163.com。陈峥，E-mail：acerdestiny@163.com。刘羽凡和陈峥为同等贡献作者。
基金资助:
国家重点研发计划(2022YFF1000700); 福建省农业科学院优秀科技创新人才专项(YCZX202408)

Biocontrol Mechanism of Bacillus-Derived Lipopeptides Through Targeted Reduction of Pathogenicity in Ralstonia solanacearum

LIU YuFan¹^,³(), CHEN Zheng²(), CHEN DeJu¹, LIU Xin¹, XIAO RongFeng¹, WANG JiePing¹, WANG Xun³, LIU Bo¹, HE Jin³(), CHEN MeiChun¹()

¹ Institute of Resources, Environment and Soil Fertilizer, Fujian Academy of Agricultural Sciences, Fuzhou 350003
² Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350003
³ National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430000

Received:2026-02-25 Accepted:2026-05-01 Published:2026-07-01 Online:2026-07-01

摘要/Abstract

摘要：

【目的】由青枯雷尔氏菌(Ralstonia solanacearum）引起的青枯病是一种破坏性严重的土传病害。芽孢杆菌脂肽具有开发为新型生物农药的潜力，但其能否通过削弱病原菌致病力发挥生防作用尚不明确。论文旨在探究脂肽降低青枯菌致病力的机制，为基于致病力调控的新型生物农药研发提供理论依据。【方法】采用倍比稀释法测定脂肽的最小抑菌浓度（MIC）；利用盆栽试验评估脂肽对青枯病的防治效果；利用试剂盒测定番茄防御相关酶活性，包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、多酚氧化酶（PPO）、过氧化物酶（POD），以及丙二醛（MDA）和过氧化氢（H₂O₂）含量；结合扫描电镜、半固体平板运动性试验及转录组测序，系统分析脂肽对青枯菌形态、运动性及基因表达的影响。【结果】芽孢杆菌FJAT-2349脂肽的MIC为0.1875 mg·mL^-1，在0.075 mg·mL^-1亚抑菌浓度下，番茄青枯病发病率相对于青枯菌对照组降低29.2%；该浓度脂肽未引起番茄叶片SOD、CAT、PPO和POD活性及MDA和H₂O₂含量的显著变化，表明其未诱导植株系统抗性。电镜观察显示，经脂肽处理的菌体伸长至正常细胞的3倍，提示细胞分裂受阻；运动性能力降低约36%。转录组分析表明，脂肽处理导致青枯菌细胞分裂基因（ZapE）、III型分泌系统调控基因（hrpB1和hrpB2）、鞭毛转录调控因子（flhC）、毒力效应蛋白基因表达显著下调，而鞭毛蛋白基因（fliC）、组氨酸利用相关酶基因（hipO）、RNA聚合酶β'亚基基因（rpoC）和细胞分裂相关基因（ftsK）表达显著上调。KEGG富集分析显示，差异表达基因主要集中于代谢（能量代谢、氨基酸代谢以及次生代谢产物生物合成）、遗传信息处理（翻译和RNA加工与代谢）和环境信息处理（ABC蛋白转运）等相关通路，表明脂肽通过干扰能量代谢和物质运输、破坏蛋白质合成与周转，重编程病原菌的代谢活动，进而抑制其生长与繁殖。【结论】亚抑菌浓度芽孢杆菌FJAT-2349脂肽主要通过双重核心机制发挥生防作用：阻滞细胞分裂进程，并抑制III型分泌系统毒力；协同干扰病菌能量代谢和物质运输、破坏蛋白质合成与周转、降低病菌运动能力，从而系统性削弱其致病力。

关键词: 芽孢杆菌, 脂肽, 青枯雷尔氏菌, 致病力, 生物防治

Abstract:

【Objective】Bacterial wilt caused by Ralstonia solanacearum is a highly destructive soil-borne disease. Lipopeptides produced by Bacillus species are promising biocontrol agents. However, whether lipopeptides can exert biocontrol effects by attenuating the pathogenicity of R. solanacearum remains unclear. This study aims to investigate the mechanism by which lipopeptides target and reduce the pathogenicity of R. solanacearum, and to provide a theoretical basis for developing novel biocontrol agents based on pathogenicity regulation.【Method】The minimum inhibitory concentration (MIC) of lipopeptides was determined using the broth dilution method. The control efficacy of lipopeptides against bacterial wilt was evaluated through pot experiments. Defense-related enzyme activities including superoxide dismutase (SOD), catalase (CAT), polyphenol oxidase (PPO), and peroxidase (POD), as well as the contents of malondialdehyde (MDA) and hydrogen peroxide (H₂O₂) in tomato plants were determined using commercial assay kits. Combined with scanning electron microscopy, semi-solid plate motility test, and transcriptome sequencing, the effects of lipopeptides on the morphology, motility, and gene expression of R. solanacearum were systematically analyzed.【Result】The MIC of lipopeptides from Bacillus sp. FJAT-2349 was 0.1875 mg·mL^-1. At a sub-inhibitory concentration (0.075 mg·mL^-1), the incidence of tomato bacterial wilt decreased by 29.2% compared to the R. solanacearum control group. No significant changes were observed in the activities of SOD, CAT, PPO, POD, or the contents of MDA and H₂O₂ in tomato leaves, indicating that this concentration of lipopeptides failed to induce systemic resistance in tomato plants. SEM analysis showed that lipopeptide-treated R. solanacearum cells exhibited an elongated rod-shaped morphology with a length approximately three times that of normal cells, suggesting impaired cell division. Motility assays showed that bacterial movement was reduced by approximately 36%. Transcriptomic analysis revealed significant down-regulation of genes associated with cell division (ZapE), type III secretion system (T3SS) regulation (hrpB1, hrpB2), flagellar transcriptional regulator (flhC) and genes encoding virulence effector proteins. The gene expressions of cell division-related enzyme (ftsK), flagellin (fliC), histidine utilization-related enzyme (hipO), and RNA polymerase β' subunit (rpoC) were up-regulated. KEGG enrichment analysis indicated that differentially expressed genes were significantly enriched in pathways related to metabolism (energy metabolism, amino acid metabolism, and biosynthesis of secondary metabolites), genetic information processing (translation and RNA processing and metabolism), and environmental information processing (ABC protein transport), suggesting that lipopeptide treatment interferes with energy metabolism and substance transport, disrupts protein synthesis and turnover, reprograms the metabolic activity of R. solanacearum, and thereby inhibits its growth and reproduction.【Conclusion】Bacillus sp. FJAT-2349 lipopeptides at sub-inhibitory concentration exert their biocontrol effect primarily through a dual-core mechanism: blocking cell division progression and suppressing T3SS virulence, which synergistically interfere with pathogen energy metabolism and substance transport, disrupt protein synthesis and turnover, and reduce pathogen motility, thereby attenuating the pathogenicity of R. solanacearum.

Key words: Bacillus, lipopeptide, Ralstonia solanacearum, pathogenicity, biocontrol

刘羽凡, 陈峥, 陈德局, 刘欣, 肖荣凤, 王阶平, 王璕, 刘波, 何进, 陈梅春. 芽孢杆菌脂肽靶向减弱青枯雷尔氏菌致病力的生防作用[J]. 中国农业科学, 2026, 59(13): 2853-2866.

LIU YuFan, CHEN Zheng, CHEN DeJu, LIU Xin, XIAO RongFeng, WANG JiePing, WANG Xun, LIU Bo, HE Jin, CHEN MeiChun. Biocontrol Mechanism of Bacillus-Derived Lipopeptides Through Targeted Reduction of Pathogenicity in Ralstonia solanacearum[J]. Scientia Agricultura Sinica, 2026, 59(13): 2853-2866.

图/表 11

表1

图1

图2

图3

图4

图5

图6

图7

图8

图9

图10

参考文献 45

[1]	Zhang W, Planas-Marquès M, Liang M, Zhang Q, Vermeulen A, Kaschani F, Kaiser M, Takken F L W, Coll N S, Valls M. The CAPE1 peptide confers resistance against bacterial wilt in tomato[J]. Journal of Experimental Botany, 2025, 76(15): 4340-4358.
[2]	张林琳, 宫瑞, 崔彦玲, 钟雄辉, 李烨, 李然红, 潜宗伟. 利用VIGS分析SmWRKY30在茄子抗青枯病中的作用[J]. 中国农业科学, 2025, 58(3): 548-563. DOI: 10.3864/j.issn.0578-1752.2025.03.011.
	Zhang L L, Gong R, Cui Y L, Zhong X H, Li Y, Li R H, Qian Z W. Effect analysis of SmWRKY30 in eggplant resistance to Ralstonia solanacearum by virus induced gene silencing (VIGS)[J]. Scientia Agricultura Sinica, 2025, 58(3): 548-563. DOI: 10.3864/j.issn.0578-1752.2025.03.011. (in Chinese)
[3]	Chachar Z, Xue X, Fang J, Chen M, Jiarui C, Chen W, Ahmed N, Chachar S, Narejo M U, Ahmed N, Fan L, Lai R, Qi Y. Key mechanisms of plant-Ralstonia solanacearum interaction in bacterial wilt pathogenesis[J]. Frontiers in Microbiology, 2025, 16: 1521422.
[4]	Moussa Z, Rashad E M, Elsherbiny E A, Al-Askar A A, Arishi A A, Al-Otibi F O, Saber W I. New strategy for inducing resistance against bacterial wilt disease using an avirulent strain of Ralstonia solanacearum[J]. Microorganisms, 2022, 10(9): 1814.
[5]	Zhu S, Chang X, Liu N, He Y, Wang J, Wu Z. The composite microbial agent controls tomato bacterial wilt by colonizing the root surface and regulating the rhizosphere soil microbial community[J]. Frontiers in Microbiology, 2025, 16: 1559380.
[6]	Mnif I, Grau-Campistany A, Coronel-León J, Hammami I, Triki M A, Manresa A, Ghribi D. Purification and identification of Bacillus subtilis SPB1 lipopeptide biosurfactant exhibiting antifungal activity against Rhizoctonia bataticola and Rhizoctonia solani[J]. Environmental Science and Pollution Research, 2016, 23(7): 6690-6699.
[7]	廖鑫琳, 郭鑫, 杨季学, 邵嘉朱, 袁歆瑜, 胡佳燕, 陈晓晓, 蒋冬花. 拮抗青枯雷尔氏菌的放线菌筛选及其防病作用[J]. 中国农业科学, 2024, 57(7): 1319-1334. DOI: 10.3864/j.issn.0578-1752.2024.07.009.
	Liao X L, Guo X, Yang J X, Shao J Z, Yuan X Y, Hu J Y, Chen X X, Jiang D H. Screening of actinomycetes against Ralstonia solanacearum and its disease prevention function[J]. Scientia Agricultura Sinica, 2024, 57(7): 1319-1334. DOI: 10.3864/j.issn.0578-1752.2024.07.009. (in Chinese)
[8]	Diniz G F, Figueiredo J E, Canuto K M, Cota L V, Souza A S, Simeone M L, Tinoco S M, Ribeiro P R, Ferreira L V, Marins M S, de Oliveira-Paiva C A, dos Santos V L. Chemical and genetic characterization of lipopeptides from Bacillus velezensis and Paenibacillus ottowii with activity against Fusarium verticillioides[J]. Frontiers in Microbiology, 2024, 15: 1443327.
[9]	Munusamy S, Conde R, Bertrand B, Munoz-Garay C. Biophysical approaches for exploring lipopeptide-lipid interactions[J]. Biochimie, 2020, 170: 173-202.
[10]	Fan S, Tian F, Fang L, Yang C H, He C. Transcriptional responses of Xanthomonas oryzae pv. oryzae to type III secretion system inhibitor ortho-coumaric acid[J]. BMC Microbiology, 2019, 19(1): 163.
[11]	Yang L, Li S, Qin X, Jiang G, Chen J, Li B, Yao X, Liang P, Zhang Y, Ding W. Exposure to umbelliferone reduces Ralstonia solanacearum biofilm formation, transcription of type III secretion system regulators and effectors and virulence on tobacco[J]. Frontiers in Microbiology, 2017, 8: 1234.
[12]	Chen K, Zhuang Y, Wang L, Li H, Lei T, Li M, Gao M, Wei J, Dang H, Raza A, Yang Q, Sharif Y, Yang H, Zhang C, Zou H, Zhuang W. Comprehensive genome sequence analysis of the devastating tobacco bacterial phytopathogen Ralstonia solanacearum strain FJ1003[J]. Frontiers in Genetics, 2022, 13: 966092.
[13]	Li S, Yu Y, Chen J, Guo B, Yang L, Ding W. Evaluation of the antibacterial effects and mechanism of action of protocatechualdehyde against Ralstonia solanacearum[J]. Molecules, 2016, 21(6): 754.
[14]	Vailleau F, Genin S. Ralstonia solanacearum: An arsenal of virulence strategies and prospects for resistance[J]. Annual Review of Phytopathology, 2023, 61: 25-47.
[15]	周池, 彭征宇, 张清壮, 彭迪, 陶禹, 李鑫. 青枯菌侵染对辣椒内生菌群落的时空动态影响及病原菌增殖特征[J]. 微生物学报, 2025, 65(11): 4921-4937.
	Zhou C, Peng Z Y, Zhang Q Z, Peng D, Tao Y, Li X. Spatiotemporal dynamics of endophytic microbiota in pepper plants infected by Ralstonia solanacearum and proliferation characteristics of the pathogen[J]. Acta Microbiologica Sinica, 2025, 65(11): 4921-4937. (in Chinese)
[16]	Yoshihara A, Shimatani M, Sakata M, Takemura C, Senuma W, Hikichi Y, Kai K. Quorum sensing inhibition attenuates the virulence of the plant pathogen Ralstonia solanacearum species complex[J]. ACS Chemical Biology, 2020, 15(11): 3050-3059.
[17]	Hendrich C G, Truchon A N, Dalsing B L, Allen C. Nitric oxide regulates the Ralstonia solanacearum type III secretion system[J]. Molecular Plant-Microbe Interactions, 2023, 36(6): 334-344.
[18]	Chen M, Zhang W, Han L, Ru X, Cao Y, Hikichi Y, Ohnishi K, Pan G, Zhang Y. A CysB regulator positively regulates cysteine synthesis, expression of type III secretion system genes, and pathogenicity in Ralstonia solanacearum[J]. Molecular Plant Pathology, 2022, 23(5): 679-692.
[19]	Tang X, Xiao Y, Zhou J M. Regulation of the type III secretion system in phytopathogenic bacteria[J]. Molecular Plant-Microbe Interactions, 2006, 19(11): 1159-1166.
[20]	Guo Q Q, Li Y Z, Shi H B, Yi A Y, Xu X L, Wang H H, Deng X, Wu Z B, Cui Z N. Novel mandelic acid derivatives suppress virulence of Ralstonia solanacearum via type III secretion system[J]. Pest Management Science, 2023, 79(11): 4626-4634.
[21]	Puigvert M, Solé M, López-Garcia B, Coll N S, Beattie K D, Davis R A, Elofsson M, Valls M. Type III secretion inhibitors for the management of bacterial plant diseases[J]. Molecular Plant Pathology, 2019, 20(1): 20-32.
[22]	郑雪芳, 王梓然, 朱育菁, 陈燕萍, 王阶平, 刘波. 不同致病力青枯雷尔氏菌诱导番茄防御相关信号途径基因的表达分析[J]. 福建农业学报, 2022, 37(1): 79-83.
	Zheng X F, Wang Z R, Zhu Y J, Chen Y P, Wang J P, Liu B. Expressions of defense signal pathway genes in tomato plant induced by Ralstonia solanacearum of different virulence[J]. Fujian Journal of Agricultural Sciences, 2022, 37(1): 79-83. (in Chinese)
[23]	Qin Q, Liu B, Ma B, Wei X, Zhou Y, Sun Z. Isolation and identification of endophytic bacterium B5 from Mentha haplocalyx Briq. and its biocontrol mechanisms against Alternaria alternata- induced tobacco brown spot [J]. Journal of Fungi, 2025, 11(6): 446.
[24]	陈娟妮, 陈品璐, 李珏, 谢蒙潇, 李欣蓓, 丁伟. 纳米氧化镁诱导烟草抗青枯病的作用机理[J]. 中国农业科学, 2025, 58(16): 3327-3344. DOI: 10.3864/j.issn.0578-1752.2025.16.015.
	Chen J N, Chen P L, Li Y, Xie M X, Li X B, Ding W. Mechanism of tobacco resistance to bacterial wilt induced by magnesium oxide nanoparticles[J]. Scientia Agricultura Sinica, 2025, 58(16): 3327-3344. DOI: 10.3864/j.issn.0578-1752.2025.16.015. (in Chinese)
[25]	Desoignies N, Schramme F, Ongena M, Legrève A. Systemic resistance induced by Bacillus lipopeptides in Beta vulgaris reduces infection by the rhizomania disease vector Polymyxa betae[J]. Molecular Plant Pathology, 2013, 14(4): 416-421.
[26]	Lam V B, Meyer T, Arias A A, Ongena M, Oni F E, Höfte M. Bacillus cyclic lipopeptides iturin and fengycin control rice blast caused by Pyricularia oryzae in potting and acid sulfate soils by direct antagonism and induced systemic resistance[J]. Microorganisms, 2021, 9(7): 1441.
[27]	Chen M C, Wang J P, Zhu Y J, Liu B, Yang W J, Ruan C Q. Antibacterial activity against Ralstonia solanacearum of the lipopeptides secreted from the Bacillus amyloliquefaciens strain FJAT-2349[J]. Journal of Applied Microbiology, 2019, 126(5): 1519-1529.
[28]	Manetsberger J, Caballero G N, Benomar N, Christie G, Abriouel H. Antimicrobial activity of environmental Bacillus spp. and Peribacillus spp. isolates linked to surfactin, fengycin, bacillibactin and lantibiotics[J]. International Journal of Biological Macromolecules, 2025, 316(1): 144644.
[29]	Charpe A M, Aglave B, Ghosh D K. Microbial-mediated induced resistance: Interactive effects for improving crop health[J]. Frontiers in Microbiology, 2025, 16: 1660944.
[30]	Balleux G, Höfte M, Arguelles-Arias A, Deleu M, Ongena M. Bacillus lipopeptides as key players in rhizosphere chemical ecology[J]. Trends in Microbiology, 2025, 33(1): 80-95.
[31]	Chen M C, Liu T T, Wang J P, Chen Y P, Chen Q X, Zhu Y J, Liu B. Strong inhibitory activities and action modes of lipopeptides on lipase[J]. Journal of Enzyme Inhibition and Medicinal Chemistry, 2020, 35(1): 897-905.
[32]	Yokota K. Bacillus cyclic lipopeptide; elicitors to induce disease resistance in biological control of plant diseases[J]. Bioscience, Biotechnology, and Biochemistry, 2026, 90(3): 313-316.
[33]	Meunier A, Cornet F, Campos M. Bacterial cell proliferation: From molecules to cells[J]. FEMS Microbiology Reviews, 2021, 45(1): fuaa046.
[34]	Marteyn B S, Karimova G, Fenton A K, Gazi A D, West N, Touqui L, Prevost M C, Betton J M, Poyraz O, Ladant D, Gerdes K, Sansonetti P J, Tang C M. ZapE is a novel cell division protein interacting with FtsZ and modulating the Z-ring dynamics[J]. mBio, 2014, 5(2): e00022-14.
[35]	Wang L, Lutkenhaus J. FtsK is an essential cell division protein that is localized to the septum and induced as part of the SOS response[J]. Molecular Microbiology, 1998, 29(3): 731-740.
[36]	Digonnet C, Martinez Y, Denancé N, Chasseray M, Dabos P, Ranocha P, Marco Y, Jauneau A, Goffner D. Deciphering the route of Ralstonia solanacearum colonization in Arabidopsis thaliana roots during a compatible interaction: Focus at the plant cell wall[J]. Planta, 2012, 236(5): 1419-1431.
[37]	Peyraud R, Cottret L, Marmiesse L, Gouzy J, Genin S. A resource allocation trade-off between virulence and proliferation drives metabolic versatility in the plant pathogen Ralstonia solanacearum[J]. PLoS Pathogens, 2016, 12(10): e1005939.
[38]	Asolkar T, Ramesh R. The involvement of the type six secretion system (T6SS) in the virulence of Ralstonia solanacearum on brinjal[J]. 3 Biotech, 2020, 10(7): 324.
[39]	Li Y, Hutchins W, Wu X, Liang C, Zhang C, Yuan X, Khokhani D, Chen X, Che Y, Wang Q, Yang C H. Derivative of plant phenolic compound inhibits the type III secretion system of Dickeya dadantii via HrpX/HrpY two-component signal transduction and Rsm systems[J]. Molecular Plant Pathology, 2015, 16(2): 150-163.
[40]	Khokhani D, Zhang C, Li Y, Wang Q, Zeng Q, Yamazaki A, Hutchins W, Zhou S S, Chen X, Yang C H. Discovery of plant phenolic compounds that act as type III secretion system inhibitors or inducers of the fire blight pathogen, Erwinia amylovora [J]. Applied and Environmental Microbiology, 2013, 79(18): 5424-5436.
[41]	Zhou Z J, Xiang L P, Wang X T, Jiang G, Cheng J, Cao X H, Fan X P, Shen H. An in-depth study of the growth inhibition of Vibrio parahaemolyticus by surfactin and its effects on cell membranes, ROS levels and gene transcription[J]. Journal of Invertebrate Pathology, 2025, 211: 108298.
[42]	Wu C Y, Huang H T, Chiang Y T, Lee K T. Surfactin inhibits enterococcal biofilm formation via interference with pilus and exopolysaccharide biosynthesis[J]. BMC Microbiology, 2025, 25: 85.
[43]	Dalsing B L, Truchon A N, Gonzalez-Orta E T, Milling A S, Allen C. Ralstonia solanacearum uses inorganic nitrogen metabolism for virulence, ATP production, and detoxification in the oxygen-limited host xylem environment[J]. mBio, 2015, 6(2): e02471.
[44]	Lowe-Power T M, Hendrich C G, von Roepenack-Lahaye E, Li B, Wu D S, Mitra R, Dalsing B L, Ricca P, Naidoo J, Cook D, Jancewicz A, Masson P, Thomma B, Lahaye T, Michael A J, Allen C. Metabolomics of tomato xylem sap during bacterial wilt reveals Ralstonia solanacearum produces abundant putrescine, a metabolite that accelerates wilt disease[J]. Environmental Microbiology, 2018, 20(4): 1330-1349.
[45]	Hamilton C D, Steidl O R, Macintyre A M, Hendrich C G, Allen C. Ralstonia solanacearum depends on catabolism of myo-inositol, sucrose, and trehalose for virulence in an infection stage-dependent manner[J]. Molecular Plant-Microbe Interactions, 2021, 34(6): 669-679.

引物名称Primer name	引物序列Primer sequence
flhC-F	ACATCTACCGTTTCCTGGGC
flhC-R	GTGCAACATCTTCCCCTCGA
fliC-F	CTGTGGAATCCAACAACGGC
fliC-R	GCTGCGTTCTGGCCATATTG
hipO-F	GCCAATATCGACCCGACCAT
hipO-R	AAGTCGTAGCTGGGGTTGTG
rpoC-F	TCGATCATCGAAACGCCGAT
rpoC-R	AATCGTCTTCCACCACCACC
recA-F	TTCTCGACCACCTTCGGCTTC
recA-R	GCTCGATCAAGAAGGGCGAT
hrpB1-F	CGAAGTGCTGATGTATGCCA
hrpB1-R	GGTCGAGCTCCTTGAAGTTC
hrpB2-F	CATGGAGGAGATCCTGGTCG
hrpB2-R	TCATTGGTTCTTCATCAAGGTCTG

芽孢杆菌脂肽靶向减弱青枯雷尔氏菌致病力的生防作用

Biocontrol Mechanism of Bacillus-Derived Lipopeptides Through Targeted Reduction of Pathogenicity in Ralstonia solanacearum

RichHTML

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 11

参考文献 45

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	刘雨晴, 张璐, 李建杰, 杨海通, 宋连杰, 李伯森, 高玉红, 张会文, 孙新胜. 高效三甲胺脱除菌株的筛选及生长条件优化[J]. 中国农业科学, 2026, 59(8): 1797-1808.
[2]	王晶, 李岗, 蔡晓雅, 庞璐瑶, 黄青滢, 林宝义, 孔海民, 郝跃, 任海英. 杨梅凋萎病生防芽孢杆菌的筛选及其防病机制[J]. 中国农业科学, 2026, 59(7): 1467-1479.
[3]	董钰, 吴千, 冯萱, 郑银英, 崔百明. 解淀粉欧文氏菌新质粒pEA60通过调节毒力因子的合成增强菌株的致病力[J]. 中国农业科学, 2026, 59(5): 996-1007.
[4]	杨帆, 胡小倩, 王宇, 岳彩霞, 张锐, 田雯, 汪婷婷, 李阳, 季美泉, 张莉会, 安可婧. 蜂蜜中需氧芽孢杆菌检测条件优化及污染特征分析[J]. 中国农业科学, 2026, 59(4): 887-899.
[5]	鄢文英, 张元珍, 吴洪鑫, 庞锐, 陈泽鹏, 金丰良, 许小霞. 靶向小菜蛾PxGNBP3的RNAi增效绿僵菌构建及其免疫调控机制[J]. 中国农业科学, 2026, 59(3): 556-574.
[6]	赵星宇, 高艳, 唐新科, 奉茵陈, 陈思颖, 顾天宇, 彭佳师. 铁在内生放线菌OsiPR-1诱导水稻稻瘟病抗性中的作用[J]. 中国农业科学, 2026, 59(13): 2828-2840.
[7]	范晓涵, 张维军, 袁锦锋, 赵栋霖, 张成省, 张之矾, 徐康文. 青枯菌GMI 1000菌株c-di-GMP合成酶Rsp1208的功能[J]. 中国农业科学, 2026, 59(11): 2374-2386.
[8]	从琪琪, 张静怡, 孟祥龙, 戴蓬博, 李波, 胡同乐, 王树桐, 曹克强, 王亚南. 我国苹果轮纹病弱毒菌株中病毒的鉴定及其携带情况的检测[J]. 中国农业科学, 2025, 58(3): 478-492.
[9]	童朝阳, 刘文华, 张国欣, 董春燕, 张燕霞, 徐晓伟, 何东, 刘何春, 李杨, 王凤涛, 冯晶, 姚小波, 刘梅金, 蔺瑞明. 青稞穗腐病发生与穗螨群体迁移的关系[J]. 中国农业科学, 2025, 58(3): 493-506.
[10]	谢海鹏, 林俊旭, 刘勇, 麦贤俊, 罗丰, 王学武, 谢文, 李少卡, 孔祥义, 吴小燕. 施用不同有机肥对贝莱斯芽孢杆菌SD13防控豇豆枯萎病的影响[J]. 中国农业科学, 2025, 58(21): 4405-4420.
[11]	胡佳燕, 沈之涵, 温丽慧, 郁嘉豪, 章雨君, 蒋冬花. 水稻细菌性条斑病菌拮抗放线菌的鉴定及其防病和促生功能[J]. 中国农业科学, 2025, 58(17): 3434-3450.
[12]	杨文娟, 高嘉诚, 王艳婷, 李妍, 郭铭, 汪军成, 孟亚雄, 王化俊, 司二静. 条纹病菌效应蛋白Pg00778调控对大麦致病力的作用[J]. 中国农业科学, 2025, 58(15): 3020-3035.
[13]	赵琳琳, 贺羽茜, 彭杰丽, 王旭, 马佳, 张秀敏, 胡栋. 链霉菌TOR3209及其挥发性有机化合物提高烟草抗木贼镰孢的能力[J]. 中国农业科学, 2025, 58(11): 2162-2175.
[14]	乔英翠, 王薄毓, 王倩, 赵丹, 郭巍, 宁文烁, 常梦颖, 王海, 陆秀君. 暗黑鳃金龟HpvATPase B克隆、表达及与Bt Cry8Ea3毒素结合特性[J]. 中国农业科学, 2025, 58(10): 1947-1957.
[15]	廖鑫琳, 郭鑫, 杨季学, 邵嘉朱, 袁歆瑜, 胡佳燕, 陈晓晓, 蒋冬花. 拮抗青枯雷尔氏菌的放线菌筛选及其防病作用[J]. 中国农业科学, 2024, 57(7): 1319-1334.