转录因子NbMYB1R1通过促进活性氧积累抑制病毒侵染

doi:10.3864/j.issn.0578-1752.2024.08.006

中国农业科学 ›› 2024, Vol. 57 ›› Issue (8): 1490-1505.doi: 10.3864/j.issn.0578-1752.2024.08.006

转录因子NbMYB1R1通过促进活性氧积累抑制病毒侵染

姜兴林¹(), 于连伟¹(), 付涵¹, 艾妞¹, 崔荧钧⁵, 李好海⁶, 夏子豪⁷, 袁虹霞¹, 李洪连¹^,³^,⁴, 杨雪¹^,²(), 施艳¹()

¹ 河南农业大学植物保护学院，郑州 450002
² 河南农业大学作物学博士后流动站，郑州 450002
³ 河南省粮食作物协同创新中心，郑州 450002
⁴ 小麦玉米作物学国家重点实验室，郑州 450002
⁵ 河南省植物保护新技术推广协会，郑州 450002
⁶ 河南省植物保护检疫站，郑州 450002
⁷ 沈阳农业大学植物保护学院，沈阳 110866

收稿日期:2023-12-06 接受日期:2024-02-04 出版日期:2024-04-16 发布日期:2024-04-24
通信作者:
杨雪，E-mail：yangxuepphappy@126.com。
施艳，E-mail：shiyan00925@126.com
联系方式: 姜兴林，E-mail：1442295312@qq.com。于连伟，E-mail：ylwnet972@163.com。姜兴林和于连伟为同等贡献作者。
基金资助:
国家自然科学基金(3210170372); 河南农业大学科技创新项目(KJCX2021A13)

The Transcription Factor NbMYB1R1 Inhibits Viral Infection by Promoting ROS Accumulation

JIANG XingLin¹(), YU LianWei¹(), FU Han¹, AI Niu¹, CUI YingJun⁵, LI HaoHai⁶, XIA ZiHao⁷, YUAN HongXia¹, LI HongLian¹^,³^,⁴, YANG Xue¹^,²(), SHI Yan¹()

¹ College of Plant Protection, Henan Agricultural University, Zhengzhou 450002
² Crop Science Postdoctoral Programme of Henan Agricultural University, Zhengzhou 450002
³ Collaborative Innovation Centre of Henan Grain Crops, Zhengzhou 450002
⁴ State Key Laboratory of Wheat and Maize Crop Science, Zhengzhou 450002
⁵ Henan Association for the Promotion of New Plant Protection Techniques, Zhengzhou 450002
⁶ Henan Plant Protection and Quarantine Station, Zhengzhou 450002
⁷ College of Plant Protection, Shenyang Agricultural University, Shenyang 110866

Received:2023-12-06 Accepted:2024-02-04 Published:2024-04-16 Online:2024-04-24

摘要/Abstract

摘要：

【背景】黄瓜绿斑驳花叶病毒（cucumber green mottle mosaic virus，CGMMV）是我国重要的检疫性植物病毒，严重降低了世界范围内蔬菜以及瓜类的产量。MYB蛋白家族庞大，功能多样，存在于所有真核生物中。大多数MYB作为转录因子控制植物的发育和代谢，并对植物应对生物和非生物胁迫反应起重要的调控作用。前期研究显示CGMMV侵染后可以显著上调寄主转录因子基因NbMYB1R1的表达。【目的】明确NbMYB1R1参与CGMMV侵染的机制，为CGMMV病害防控提供理论依据。【方法】运用MEGA 7.0构建系统进化树，对NbMYB1R1的氨基酸序列进行系统进化分析；通过构建NbMYB1R1的荧光表达载体，转化GV3101农杆菌后浸润本氏烟叶片，激光共聚焦显微镜观察其亚细胞定位；利用qRT-PCR技术分析NbMYB1R1在CGMMV侵染不同时期的转录水平及NbMYB1R1沉默烟草植株中活性氧（reactive oxygen species，ROS）相关基因的转录变化；通过沉默NbMYB1R1及下游调控基因NbAOX1a和NbAOX1b，分析NbMYB1R1及下游调控基因NbAOX1a和NbAOX1b在CGMMV侵染过程中的作用；瞬时过表达NbMYB1R1和NbMYB1R1关键氨基酸突变体，分析NbMYB1R1对CGMMV侵染的影响；使用台盼蓝染色以及DAB染色观察瞬时过表达NbMYB1R1造成的细胞死亡是否与程序性细胞死亡（programmed cell death，PCD）以及ROS的积累有关；运用酵母双杂交技术验证NbMYB1R1是否与CGMMV相关蛋白互作。【结果】系统进化树分析表明，NbMYB1R1属于1R MYB大类并与多种烟草的MYB转录因子同源性极高；亚细胞定位结果显示NbMYB1R1定位于细胞核；在CGMMV侵染的烟草植株中，NbMYB1R1的转录水平对比健康植株有明显变化，在CGMMV侵染8、12 d时NbMYB1R1的转录水平显著上调；在沉默内源基因NbMYB1R1的植株上接种CGMMV，3 d后NbMYB1R1沉默植株系统叶出现斑驳、卷曲症状，而对照植株在3.5 d时才出现症状；同时CGMMV CP mRNA水平和蛋白水平检测结果也表明沉默NbMYB1R1可以有效促进CGMMV的积累；在本氏烟叶片瞬时过表达NbMYB1R1及其突变体3 d时检测CGMMV蛋白水平表达量，结果显示过表达NbMYB1R1可以有效抑制CGMMV的侵染，DNA结合结构域缺失后会减轻NbMYB1R1对CGMMV的抑制；台盼蓝和DAB染色结果表明，在瞬时过表达NbMYB1R1蛋白后导致ROS积累并引起细胞死亡；在沉默内源基因NbMYB1R1的植株叶片上检测ROS相关基因的转录水平，发现交替氧化酶基因AOX1a、AOX1b转录水平显著上调；在沉默内源基因NbAOX1a和NbAOX1b的植株上接种CGMMV，4 d后NbAOX1a和NbAOX1b沉默植株系统叶出现斑驳、卷曲症状，而对照植株在3.5 d时就出现症状；同时CGMMV CP mRNA水平和蛋白水平检测结果也表明沉默NbAOX1a和NbAOX1b可以有效抑制CGMMV的积累；酵母双杂交结果显示NbMYB1R1不与CGMMV编码蛋白直接相互作用。【结论】随着CGMMV侵染，防御相关基因NbMYB1R1表达上调，从而抑制下游基因AOX1a、AOX1b的表达并激活细胞内产生ROS抑制病毒侵染，但NbMYB1R1不是通过与CGMMV病毒蛋白直接互作而产生此作用。由此可见，NbMYB1R1在CGMMV侵染过程中发挥了重要作用。

关键词: 黄瓜绿斑驳花叶病毒, NbMYB1R1, 转录因子, 致病机制, 活性氧

Abstract:

【Background】Cucumber green mottle mosaic virus (CGMMV) is an important quarantine plant virus in China, which has seriously reduced the production of vegetables and melons worldwide. The MYB protein family is large, multifunctional and present in all eukaryotes. Most MYB proteins act as transcription factors that control development, and metabolism in plants, and regulate biotic and abiotic stress responses. Previous studies have shown that during CGMMV infection, the expression of transcription factor gene NbMYB1R1 was significantly up-regulated. 【Objective】The objective of this study is to clarify the mechanism of NbMYB1R1 involved in CGMMV infection, and to provide a theoretical basis for controlling CGMMV infection. 【Method】MEGA 7.0 was used to construct a phylogenetic tree to analyze the amino acid sequence of NbMYB1R1 protein. The expression vector NbMYB1R1-GFP was constructed, transformed into Agrobacterium GV3101 and infiltrated the tobacco leaves to observe the subcellular localization of NbMYB1R1 by confocal microscopy. The transcriptional levels of NbMYB1R1 at different stages of CGMMV infection and ROS related genes in silenced NbMYB1R1 plants were analyzed using qRT-PCR technology. TRV-mediated gene silencing (VIGS) was utilized to analyze the function of NbMYB1R1, NbAOX1a, and NbAOX1b during CGMMV infection. Transient overexpression of NbMYB1R1 and NbMYB1R1 mutant was conducted to analyze the effect of NbMYB1R1 on CGMMV infection. Trypan blue staining and DAB staining were used to observe whether the cell death caused by the transient overexpression of NbMYB1R1 was related to the programmed cell death (PCD) and the accumulation of ROS. Yeast two-hybrid system was used to verify whether NbMYB1R1 interacted with CGMMV related proteins. 【Result】Phylogenetic tree analysis showed that NbMYB1R1 belonged to the 1R MYB category and had high homology with MYB transcription factors in a variety of tobaccos. The results of subcellular localization showed that NbMYB1R1 was localized in the nucleus. In CGMMV-infected tobacco plants, the transcript level of NbMYB1R1 was significantly changed compared with healthy plants, which was significantly up-regulated at 8 and 12 d of CGMMV infection. CGMMV was inoculated into TRV:NbMYB1R1 and TRV:00 plants. The NbMYB1R1-silenced plants showed the mottled and curled symptoms at 3 dpi, while the control plant appeared the symptoms at 3.5 dpi. Silencing NbMYB1R1 could effectively promote the accumulation of CGMMV at mRNA and protein levels. The CGMMV accumulation in NbMYB1R1 and NbMYB1R1 mutant transiently overexpression leaves was detected, and the results showed that NbMYB1R1 overexpression could effectively inhibit CGMMV infection and DNA binding domain deletion mutant reduced the inhibition of CGMMV by NbMYB1R1. The results of trypan blue and DAB staining showed that NbMYB1R1 overexpression could induce the ROS accumulation and cell death. The transcription level of ROS-related genes in TRV:NbMYB1R1 and TRV:00 plant was also detected, and the results showed that silencing NbMYB1R1 could specifically up-regulate the transcription of AOX1a and AOX1b. After CGMMV inoculation on silencing endogenous genes NbAOX1a and NbAOX1b, the systemic leaves of silenced NbAOX1a and NbAOX1b plants showed mottled and curled symptoms at 4 dpi, while the control plants appeared symptoms at 3.5 d. Meanwhile, the results of CGMMV CP mRNA and protein levels also indicated that silencing NbAOX1a and NbAOX1b could effectively inhibit the accumulation of CGMMV. The yeast two-hybrid results showed that NbMYB1R1 did not directly interact with the CGMMV proteins. 【Conclusion】During the CGMMV infection, the defense-related gene NbMYB1R1 is up-regulated. It speculates that the upregulation of NbMYB1R1 inhibits the transcription of the downstream genes AOX1a and AOX1b and activates the production of ROS in cells, thereby inhibiting viral infection. However, NbMYB1R1 does not produce this effect through a direct interaction with the CGMMV viral protein. NbMYB1R1 plays an important role in CGMMV infection.

Key words: cucumber green mottle mosaic virus (CGMMV), NbMYB1R1, transcription factor, pathogenic mechanism, reactive oxygen species (ROS)

姜兴林, 于连伟, 付涵, 艾妞, 崔荧钧, 李好海, 夏子豪, 袁虹霞, 李洪连, 杨雪, 施艳. 转录因子NbMYB1R1通过促进活性氧积累抑制病毒侵染[J]. 中国农业科学, 2024, 57(8): 1490-1505.

JIANG XingLin, YU LianWei, FU Han, AI Niu, CUI YingJun, LI HaoHai, XIA ZiHao, YUAN HongXia, LI HongLian, YANG Xue, SHI Yan. The Transcription Factor NbMYB1R1 Inhibits Viral Infection by Promoting ROS Accumulation[J]. Scientia Agricultura Sinica, 2024, 57(8): 1490-1505.

图/表 11

表1

本研究所用引物"

用途Use	引物名称Primer name	引物序列Primer sequence (5′-3′)
酵母双杂交系统 Yeast two-hybrid	pGADT7- NbMYB1R1-F	ATATGGCCATGGAGGCCAGTGAATTCATGTCTAACGAATGCGGAAACAAAGA
	pGADT7- NbMYB1R1-R	ATCTGCAGCTCGAGCTCGATGGATCCAGCCACACTAATTATGCTATCCCC
	pGBKT7-CP-F	TGCATATGGCCATGGAGGCCGAATTCATGGCTTACAATCCGATCACACCTA
	pGBKT7-CP-R	TGCGGCCGCTGCAGGTCGACGGATCCAGCTTTCGAGGTGGTAGCC
	pGBKT7-MP-F	TGCATATGGCCATGGAGGCCGAATTCATGTCTCTAAGTAAGGTGTCAGTCG
	pGBKT7-MP-R	TGCGGCCGCTGCAGGTCGACGGATCCGGTGTGATCGGATTGTAAGCC
	pGBKT7-129K-F	TGCATATGGCCATGGAGGCCGAATTCATGGCAAACATTAATGAACAAATCA
	pGBKT7-129K-R	TGCGGCCGCTGCAGGTCGACGGATCCTTTGGTAGGCACAGTGGTAGC
	pGBKT7-MET-F	TGCATATGGCCATGGAGGCCGAATTCATGGCAAACATTAATGAACAAATCA
	pGBKT7-MET-R	TGCGGCCGCTGCAGGTCGACGGATCCCTCGATCTGTACCTTCCTAACCTTCA
	pGBKT7-HEL-F	TGCATATGGCCATGGAGGCCGAATTCACATTAGTTGACGGAGTGCC
	pGBKT7-HEL-R	TGCGGCCGCTGCAGGTCGACGGATCCTTTGGTAGGCACAGTGGTAGC
	pGBKT7-P2-F	TGCATATGGCCATGGAGGCCGAATTCCAATTAATGCAGAACTCGCTGTATG
	pGBKT7-P2-R	TGCGGCCGCTGCAGGTCGACGGATCCCTTAGAGACATCTATGTAAAGACTAC
瞬时过表达 Over-expression	NbMYB1R1-GFP-F	ACGAGCTGTACAAGGGTACCATGTCTAACGAATGCGGAAACAAAGA
	NbMYB1R1-GFP-R	CGGACTCTAGTTCATCTAGAAGCCACACTAATTATGCTATCCCC
	NbMYB1R1^121-127-GFP-F	CCTACTCAGGTGGCCCTCCGCCGAAATAACATCAA
	NbMYB1R1^121-127-GFP-R	GTTATTTCGGCGGAGGGCCACCTGAGTAGGGGTC
基因沉默 Virus induced gene silencing (VIGS)	NbMYB1R1 VIGS-F	AGTGGTCTCTGTCCAGTCCTCAAGACAAGGACCCCTACTC
	NbMYB1R1 VIGS-R	GGTCTCAGCAGACCACAAGTGGTATTGGCCTAATGGGCT
	NbAOX1a/b VIGS-F	AGTGGTCTCTGTCCAGTCCT GTAGGAGGAATGTTGTTGCACT
	NbAOX1a/b VIGS-R	GGTCTCAGCAGACCACAAGT ATTACCCTTGTCTAATTCCTTGAGG
实时荧光定量PCR Quantitative real-time PCR	NbUBC-qPCR-F	TTTCGGTCCTGATGATACTCCC
	NbUBC-qPCR-R	CACAGAGCAAAGACTGGATTGA
	NbMYB1R1-qPCR-F	GGGTTCATGCTCTTTGGTGT
	NbMYB1R1-qPCR-R	ACGGGTCCTAGCAGAAGGAT
	NbAOX1a-qPCR-F	TTGAGAACGTTCCTGCTCCT
	NbAOX1a-qPCR-R	TGGAGAGTCCCTTGAGCTGT
	NbAOX1b-qPCR-F	TTGAGAACGTTCCTGCTCCT
	NbAOX1b-qPCR-R	GGTGCTGGAGAGTCCTTGAG
	NbGST-qPCR-F	TGAAGGAAAGCGAAGCACAAT
	NbGST-qPCR-R	ACGACGGCGCGAATCAAACA
	NbSOD-qPCR-F	GCCGTCCTTAGCAGCAGTGAA
	NbSOD-qPCR-R	CCGGGTTTTAGGCCAGAGACAT
	NbAPX1-qPCR-F	GGAGTGGTTGCTGTTGAAGTC
	NbAPX1-qPCR-R	GGAGAGCCTTGTCTGATGG
	NbCAT3-qPCR-F	TGCGCATCACAATAATCACCAT
	NbCAT3-qPCR-R	TGTCGCGCTTTCCTGTCAA
	NbGPX2-qPCR-F	TTGTTTTGCCACTACCTTGTTCAG
	NbGPX2-qPCR-R	TTCATTTGGGGGTGTCAGATTAG
	CP-qPCR-F	ACAAGGTACCGCTTTCCAGA
	CP-qPCR-R	TACGACAGACGAGGGTAACG

表1

表2

图1

图2

图3

图4

图5

图6

图7

图8

图9

参考文献 49

[1]	赵文. 黄瓜栽培的现状及其发展趋势. 智慧农业导刊, 2022, 2(3): 32-34.
	ZHAO W. The current situation and development trends of cucumber cultivation. Journal of Smart Agriculture, 2022, 2(3): 32-34. (in Chinese)
[2]	LING K S, LI R, ZHANG W. First report of cucumber green mottle mosaic virus infecting greenhouse cucumber in Canada. Plant Disease, 2014, 98(5): 701.
[3]	KLEMPNAUER K H, GONDA T J, BISHOP J M. Nucleotide sequence of the retroviral leukemia gene v-myb and its cellular progenitor c-myb: The architecture of a transduced oncogene. Cell, 1982, 31(1): 453-463. doi: 10.1016/0092-8674(82)90138-6
[4]	PAZ-ARES J, GHOSAL D, WIENAND U, PETERSON P A, SAEDLER H. The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators. The EMBO Journal, 1987, 6(12): 3553-3558. doi: 10.1002/embj.1987.6.issue-12
[5]	KATIYAR A, SMITA S, LENKA S K, RAJWANSHI R, CHINNUSAMY V, BANSAL K C. Genome-wide classification and expression analysis of MYB transcription factor families in rice and Arabidopsis. BMC Genomics, 2012, 13: 544. doi: 10.1186/1471-2164-13-544
[6]	SALIH H, GONG W, HE S, SUN G, SUN J, DU X. Genome- wide characterization and expression analysis of MYB transcription factors in Gossypium hirsutum. BMC Genetics, 2016, 17(1): 129. doi: 10.1186/s12863-016-0436-8
[7]	WEI Q, CHEN R, WEI X, LIU Y, ZHAO S, YIN X, XIE T. Genome- wide identification of R2R3-MYB family in wheat and functional characteristics of the abiotic stress responsive gene TaMYB344. BMC Genomics, 2020, 21(1): 792. doi: 10.1186/s12864-020-07175-9
[8]	ZHAO P, LI Q, LI J, WANG L, REN Z. Genome-wide identification and characterization of R2R3MYB family in Solanum lycopersicum. Molecular Genetics and Genomics, 2014, 289(6): 1183-1207. doi: 10.1007/s00438-014-0879-4
[9]	CHEN G, HE W, GUO X, PAN J. Genome-wide identification, classification and expression analysis of the MYB transcription factor family in Petunia. International Journal of Molecular Sciences, 2021, 22(9): 4838. doi: 10.3390/ijms22094838
[10]	OGATA K, MORIKAWA S, NAKAMURA H, SEKIKAWA A, INOUE T, KANAI H, SARAI A, ISHII S, NISHIMURA Y. Solution structure of a specific DNA complex of the Myb DNA-binding domain with cooperative recognition helices. Cell, 1994, 79(4): 639-648. doi: 10.1016/0092-8674(94)90549-5 pmid: 7954830
[11]	DUBOS C, STRACKE R, GROTEWOLD E, WEISSHAAR B, MARTIN C, LEPINIEC L. MYB transcription factors in Arabidopsis. Trends in Plant Science, 2010, 15(10): 573-581. doi: 10.1016/j.tplants.2010.06.005
[12]	YU E Y, KIM S E, KIM J H, KO J H, CHO M H, CHUNG I K. Sequence-specific DNA recognition by the Myb-like domain of plant telomeric protein RTBP1. The Journal of Biological Chemistry, 2000, 275(31): 24208-24214. doi: 10.1074/jbc.M003250200
[13]	HAGA N, KATO K, MURASE M, ARAKI S, KUBO M, DEMURA T, SUZUKI K, MÜLLER I, VOß U, JÜRGENS G, ITO M. R1R2R3-Myb proteins positively regulate cytokinesis through activation of KNOLLE transcription in Arabidopsis thaliana. Development, 2007, 134(6): 1101-1110. doi: 10.1242/dev.02801
[14]	HOU X J, LI S B, LIU S R, HU C G, ZHANG J Z. Genome-wide classification and evolutionary and expression analyses of citrus MYB transcription factor families in sweet orange. PLoS ONE, 2014, 9(11): e112375. doi: 10.1371/journal.pone.0112375
[15]	MATUS J T, AQUEA F, ARCE-JOHNSON P. Analysis of the grape MYB R2R3 subfamily reveals expanded wine quality-related clades and conserved gene structure organization across Vitis and Arabidopsis genomes. BMC Plant Biology, 2008, 8(1): 83. doi: 10.1186/1471-2229-8-83
[16]	WILKINS O, NAHAL H, FOONG J, PROVART N J, CAMPBELL M M. Expansion and diversification of the Populus R2R3-MYB family of transcription factors. Plant Physiology, 2009, 149(2): 981-993. doi: 10.1104/pp.108.132795
[17]	LIU Z, LUAN Y, LI J, YIN Y. Expression of a tomato MYB gene in transgenic tobacco increases resistance to Fusarium oxysporum and Botrytis cinerea. European Journal of Plant Pathology, 2016, 144(3): 607-617. doi: 10.1007/s10658-015-0799-0
[18]	LIU T, CHEN T, KAN J, YAO Y, GUO D, YANG Y, LING X, WANG J, ZHANG B. The GhMYB36 transcription factor confers resistance to biotic and abiotic stress by enhancing PR1 gene expression in plants. Plant Biotechnology Journal, 2022, 20(4): 722-735. doi: 10.1111/pbi.v20.4
[19]	XIAO S, HU Q, SHEN J, LIU S, YANG Z, CHEN K, KLOSTERMAN S J, JAVORNIK B, ZHANG X, ZHU L. GhMYB4 downregulates lignin biosynthesis and enhances cotton resistance to Verticillium dahliae. Plant Cell Reports, 2021, 40(4): 735-751. doi: 10.1007/s00299-021-02672-x
[20]	QIU B, CHEN H, ZHENG L, SU L, CUI X, GE F, LIU D. An MYB transcription factor modulates Panax notoginseng resistance against the root rot pathogen Fusarium solani by regulating the jasmonate acid signaling pathway and photosynthesis. Phytopathology, 2022, 112(6): 1323-1334. doi: 10.1094/PHYTO-07-21-0283-R
[21]	CUI J, JIANG N, ZHOU X, HOU X, YANG G, MENG J, LUAN Y. Tomato MYB49 enhances resistance to Phytophthora infestans and tolerance to water deficit and salt stress. Planta, 2018, 248(6): 1487-1503. doi: 10.1007/s00425-018-2987-6
[22]	QIU Z, YAN S, XIA B, JIANG J, YU B, LEI J, CHEN C, CHEN L, YANG Y, WANG Y, TIAN S, CAO B. The eggplant transcription factor MYB44 enhances resistance to bacterial wilt by activating the expression of spermidine synthase. Journal of Experimental Botany, 2019, 70(19): 5343-5354. doi: 10.1093/jxb/erz259 pmid: 31587071
[23]	LIU R, CHEN L, JIA Z, LÜ B, SHI H, SHAO W, DONG H. Transcription factor AtMYB44 regulates induced expression of the ETHYLENE INSENSITIVE2 gene in Arabidopsis responding to a harpin protein. Molecular Plant-Microbe Interactions, 2011, 24(3): 377-389. doi: 10.1094/MPMI-07-10-0170
[24]	YANG Y, KLESSIG D F. Isolation and characterization of a tobacco mosaic virus-inducible myb oncogene homolog from tobacco. Proceedings of the National Academy of Sciences of the United States of America, 1996, 93(25): 14972-14977.
[25]	ZHU T, ZHOU X, ZHANG J L, ZHANG W H, ZHANG L P, YOU C X, JAMESON P E, MA P T, GUO S L. Ethylene-induced NbMYB4L is involved in resistance against tobacco mosaic virus in Nicotiana benthamiana. Molecular Plant Pathology, 2022, 23(1): 16-31. doi: 10.1111/mpp.13139
[26]	ZHOU H, PENG Q, ZHAO J, OWITI A, REN F, LIAO L, WANG L, DENG X, JIANG Q, HAN Y. Multiple R2R3-MYB transcription factors involved in the regulation of anthocyanin accumulation in peach flower. Frontiers in Plant Science, 2016, 7: 1557. pmid: 27818667
[27]	KOUNDAL V, VINUTHA T, HAQ Q M R, PRAVEEN S. Modulation of plant development and MYB down regulation: Both during in planta expression of miR159a and in natural ToLCV infection. Journal of Plant Biochemistry and Biotechnology, 2010, 19: 171-175. doi: 10.1007/BF03263337
[28]	SEO P J, XIANG F, QIAO M, PARK J Y, LEE Y N, KIM S G, LEE Y H, PARK W J, PARK C M. The MYB96 transcription factor mediates abscisic acid signaling during drought stress response in Arabidopsis. Plant Physiology, 2009, 151(1): 275-289. doi: 10.1104/pp.109.144220
[29]	SLAVOKHOTOVA A, KOROSTYLEVA T, SHELENKOV A, PUKHALSKIY V, KOROTTSEVA I, SLEZINA M, ISTOMINA E, ODINTSOVA T. Transcriptomic analysis of genes involved in plant defense response to the cucumber green mottle mosaic virus infection. Life, 2021, 11(10): 1064. doi: 10.3390/life11101064
[30]	EULGEM T. Regulation of the Arabidopsis defense transcriptome. Trends in Plant Science, 2005, 10(2): 71-78. doi: 10.1016/j.tplants.2004.12.006
[31]	YU Y, ZHANG S, YU Y, CUI N, YU G, ZHAO H, MENG X, FAN H. The pivotal role of MYB transcription factors in plant disease resistance. Planta, 2023, 258(1): 16. doi: 10.1007/s00425-023-04180-6 pmid: 37311886
[32]	VAILLEAU F, DANIEL X, TRONCHET M, MONTILLET J L, TRIANTAPHYLIDÈS C, ROBY D. A R2R3-MYB gene, AtMYB30, acts as a positive regulator of the hypersensitive cell death program in plants in response to pathogen attack. Proceedings of the National Academy of Sciences of the United States of America, 2002, 99(15): 10179-10184.
[33]	RAFFAELE S, VAILLEAU F, LÉGER A, JOUBÈS J, MIERSCH O, HUARD C, BLÉE E, MONGRAND S, DOMERGUE F, ROBY D. A MYB transcription factor regulates very-long-chain fatty acid biosynthesis for activation of the hypersensitive cell death response in Arabidopsis. The Plant Cell, 2008, 20(3): 752-767. doi: 10.1105/tpc.107.054858
[34]	NOMAN A, HUSSAIN A, ADNAN M, KHAN M I, ASHRAF M F, ZAINAB M, KHAN K A, GHRAMH H A, HE S. A novel MYB transcription factor CaPHL8 provide clues about evolution of pepper immunity againstsoil borne pathogen. Microbial Pathogenesis, 2019, 137: 103758. doi: 10.1016/j.micpath.2019.103758
[35]	YAO L, YANG B, XIAN B, CHEN B, YAN J, CHEN Q, GAO S, ZHAO P, HAN F, XU J, JIANG Y Q. The R2R3-MYB transcription factor BnaMYB111L from rapeseed modulates reactive oxygen species accumulation and hypersensitive-like cell death. Plant Physiology and Biochemistry, 2020, 147: 280-288. doi: S0981-9428(19)30543-1 pmid: 31891862
[36]	DANEVA A, GAO Z, VAN DURME M, NOWACK M K. Functions and regulation of programmed cell death in plant development. Annual Review of Cell and Developmental Biology, 2016, 32: 441-468. pmid: 27298090
[37]	CHEN B, NIU F, LIU W Z, YANG B, ZHANG J, MA J, CHENG H, HAN F, JIANG Y Q. Identification, cloning and characterization of R2R3-MYB gene family in canola (Brassica napus L.) identify a novel member modulating ROS accumulation and hypersensitive-like cell death. DNA Research, 2016, 23(2): 101-114. doi: 10.1093/dnares/dsv040
[38]	NG D W, ABEYSINGHE J K, KAMALI M. Regulating the regulators: The control of transcription factors in plant defense signaling. International Journal of Molecular Sciences, 2018, 19(12): 3737. doi: 10.3390/ijms19123737
[39]	VISWANATH K K, KUO S Y, TU C W, HSU Y H, HUANG Y W, HU C C. The role of plant transcription factors in the fight against plant viruses. International Journal of Molecular Sciences, 2023, 24(9): 8433. doi: 10.3390/ijms24098433
[40]	李强, 康璠, 薛晴, 陈斌, 孙颖. 神农香菊R2R3-MYB转录因子CiMYB4在镉胁迫中的功能分析. 草业学报, 2024, 33(5): 128-142. doi: 10.11686/cyxb2023236
	LI Q, KANG F, XUE Q, CHEN B, SUN Y. Functional analysis of the R2R3-MYB transcription factor CiMYB4 of Chrysanthemum indicum var. aromaticum in response to cadmium stress. Acta Prataculturae Sinica, 2024, 33(5): 128-142. (in Chinese)
[41]	解振强, 许桓瑜, 黄金霞, 蔡善亚, 李刚, 赵鹏程. 葡萄MYB转录因子基因VvMYB30的克隆及其耐盐性分析. 中外葡萄与葡萄酒, 2024(1): 20-27.
	XIE Z Q, XU H Y, HUANG J X, CAI S Y, LI G, ZHAO P C. Cloning and salt tolerance analysis of VvMYB30 from grape (V. vinifera). Sino-Overseas Grapevine & Wine, 2024(1): 20-27. (in Chinese)
[42]	姚利晓, 范海芳, 张庆雯, 何永睿, 许兰珍, 雷天刚, 彭爱红, 李强, 邹修平, 陈善春. 柑橘溃疡病抗性相关转录因子CitMYB20的功能. 中国农业科学, 2020, 53(10): 1997-2008. doi: 10.3864/j.issn.0578-1752.2020.10.007.
	YAO L X, FAN H F, ZHANG Q W, HE Y R, XU L Z, LEI T G, PENG A H, LI Q, ZOU X P, CHEN S C. Function of citrus bacterial canker resistance-related transcription factor CitMYB20. Scientia Agricultura Sinica, 2020, 53(10): 1997-2008. doi: 10.3864/j.issn.0578-1752.2020.10.007. (in Chinese)
[43]	YANG L L, LI Q L, HAN X Y, JIANG X L, WANG H, SHI Y J, CHEN L L, LI H L, LIU Y Q, YANG X, SHI Y. A cysteine-rich secretory protein involves in phytohormone melatonin mediated plant resistance to CGMMV. BMC Plant Biology, 2023, 23(1): 215. doi: 10.1186/s12870-023-04226-7
[44]	LI Y, ZHANG R, WU Y, WU Q, JIANG Q, MA J, ZHANG Y, QI P, CHEN G, JIANG Y, ZHENG Y, WEI Y, XU Q. TaRBP1 stabilizes TaGLTP and negatively regulates stripe rust resistance in wheat. Molecular Plant Pathology, 2023, 24(10): 1205-1219. doi: 10.1111/mpp.13364 pmid: 37306522
[45]	陈同睿, 罗艳君, 赵潘婷, 贾海燕, 马正强. 过表达TaJRL53基因提高了小麦赤霉病抗性. 作物学报, 2021, 47(1): 19-29. doi: 10.3724/SP.J.1006.2021.01050
	CHEN T R, LUO Y J, ZHAO P T, JIA H Y, MA Z Q. Overexpression of TaJRL53 enhances the Fusarium head blight resistance in wheat. Acta Agronomica Sinica, 2021, 47(1): 19-29. (in Chinese) doi: 10.3724/SP.J.1006.2021.01050
[46]	TIAN Z, ZHANG Z, KANG L, LI M, ZHANG J, FENG Y, YIN J, GONG X, ZHAO J. Small G protein StRab5b positively regulates potato resistance to Phytophthora infestans. Frontiers in Plant Science, 2022, 13: 1065627. doi: 10.3389/fpls.2022.1065627
[47]	CVETKOVSKA M, VANLERBERGHE G C. Alternative oxidase impacts the plant response to biotic stress by influencing the mitochondrial generation of reactive oxygen species. Plant, Cell and Environment, 2013, 36(3): 721-732. doi: 10.1111/pce.2013.36.issue-3
[48]	ZHANG X, IVANOVA A, VANDEPOELE K, RADOMILJAC J, VAN DE VELDE J, BERKOWITZ O, WILLEMS P, XU Y, NG S, VAN AKEN O, et al. The transcription factor MYB 29 is a regulator of ALTERNATIVE OXIDASE1a. Plant Physiology, 2017, 173(3): 1824-1843. doi: 10.1104/pp.16.01494
[49]	GONG Q, LI S, ZHENG Y, DUAN H, XIAO F, ZHUANG Y, HE J, WU G, ZHAO S, ZHOU H, LIN H. SUMOylation of MYB30 enhances salt tolerance by elevating alternative respiration via transcriptionally upregulating AOX1a in Arabidopsis. The Plant Journal, 2020, 102(6): 1157-1171. doi: 10.1111/tpj.v102.6

转录因子NbMYB1R1通过促进活性氧积累抑制病毒侵染

The Transcription Factor NbMYB1R1 Inhibits Viral Infection by Promoting ROS Accumulation

RichHTML

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 11

参考文献 49

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	曹海顺, 周东源, 王瑞, 施招婉, 吴廷全, 张长远. 弱光胁迫短下胚轴黄瓜种质鉴定及其遗传位点挖掘[J]. 中国农业科学, 2026, 59(6): 1286-1301.
[2]	付涵, 于杨, 艾妞, 张思晴, 于连伟, 孙书豪, 赵金章, 韩晓玉, 施艳, 杨雪. 光合作用系统Ⅱ相关蛋白NbPsbQ1通过促进光合效率抑制病毒侵染[J]. 中国农业科学, 2026, 59(1): 90-100.
[3]	韦萍, 潘炬忠, 朱德平, 邵胜雪, 陈珊珊, 韦雅倩, 高维维. OsDREB1J调控水稻籽粒大小的功能研究[J]. 中国农业科学, 2025, 58(8): 1463-1478.
[4]	王梦园, 魏倩睿, 李海艳, 杨巧敏, 宇军, 黄炜, 逯明辉. 辣椒MADS-box转录因子基因CaAGL61的耐热功能分析[J]. 中国农业科学, 2025, 58(8): 1604-1616.
[5]	潘丽媛, 王永军, 李海军, 侯富, 李菁, 李丽丽, 孙苏阳. 基于转录组和WGCNA筛选小麦籽粒蛋白质累积相关调控基因[J]. 中国农业科学, 2025, 58(6): 1065-1082.
[6]	刁邓超, 李云丽, 孟祥宇, 季颂涵, 孙玉晨, 马学红, 李杰, 冯永佳, 李春莲, 吴建辉, 曾庆东, 韩德俊, $\boxed{\hbox{王长发}}$, 郑炜君. 小麦TaGRAS34-5A的克隆及耐热功能研究[J]. 中国农业科学, 2025, 58(4): 617-634.
[7]	张林琳, 宫瑞, 崔彦玲, 钟雄辉, 李烨, 李然红, 潜宗伟. 利用VIGS分析SmWRKY30在茄子抗青枯病中的作用[J]. 中国农业科学, 2025, 58(3): 548-563.
[8]	邹霈怡, 刘美艳, 王颖, 李然红. 狗枣猕猴桃AkNAC2的克隆及功能研究[J]. 中国农业科学, 2025, 58(19): 3985-3999.
[9]	阮桥君, 毛苗苗, 张媛媛, 林晓蓉, 陈忠正. 茶树‘英红9号’乙胺合成关键酶基因CsAlaDC转录因子的筛选[J]. 中国农业科学, 2025, 58(12): 2427-2438.
[10]	赵守帅, 都梦翔, 郭思宇, 张烁, 赵宏伟, 赵长江. 钙调节活性氧和内生细菌增强水稻对纹枯病抗性[J]. 中国农业科学, 2025, 58(11): 2145-2161.
[11]	李凯利, 魏云晓, 种智力, 孟志刚, 王远, 梁成真, 陈全家, 张锐. 红蓝光促进陆地棉愈伤组织诱导和增殖[J]. 中国农业科学, 2024, 57(4): 638-649.
[12]	何勇, 范晓珠, 陈新月, 段书静, 胡婷婷, 谢如雪, 王宇晴, 陈静. 利用酵母双杂交系统筛选与辣椒轻斑驳病毒126 kDa蛋白互作的辣椒寄主因子[J]. 中国农业科学, 2024, 57(15): 2986-2996.
[13]	胡丹丹, 罗润琪, 梁瑞英, 汪磊, 梁琳, 司红彬, 丁家波, 汤新明. ApiAP2转录因子家族调控弓形虫生长发育的研究进展[J]. 中国农业科学, 2024, 57(13): 2687-2697.
[14]	赵风云, 程志虹, 谭强飞, 朱嘉宁, 孙万合, 张紊玮, 贠建民. 甘露醇对草菇继代菌株生产性状和ROS清除能力的影响[J]. 中国农业科学, 2024, 57(1): 190-203.
[15]	李慧, 张雨峰, 李晓刚, 王中华, 蔺经, 常有宏. 全基因组DNA甲基化和转录组联合分析鉴定杜梨耐盐相关转录因子[J]. 中国农业科学, 2023, 56(7): 1377-1390.