盐胁迫下枯草芽孢杆菌NCD-2对番茄促生作用及对土壤微生物群落结构的影响

doi:10.3864/j.issn.0578-1752.2021.21.008

中国农业科学 ›› 2021, Vol. 54 ›› Issue (21): 4573-4584.doi: 10.3864/j.issn.0578-1752.2021.21.008

盐胁迫下枯草芽孢杆菌NCD-2对番茄促生作用及对土壤微生物群落结构的影响

邵美琪^1,²(),赵卫松²,苏振贺²,董丽红²,郭庆港^2,^*(),马平²

¹河北农业大学植物保护学院,河北保定 071001
²河北省农林科学院植物保护研究所/河北省农业有害生物综合防治工程技术研究中心/农业农村部华北北部作物有害生物综合治理重点实验室,河北保定 071000

收稿日期:2021-03-17 接受日期:2021-03-31 出版日期:2021-11-01 发布日期:2021-11-09
通讯作者: 郭庆港
作者简介:联系方式：邵美琪,E-mail： 18849208821@163.com。
基金资助:
国家自然科学基金(31572051);河北省重点研发计划(19226510D);河北省农林科学院现代农业科技创新工程(2019-1-2-3);河北省自然科学基金(C2019301101)

Effect of Bacillus subtilis NCD-2 on the Growth of Tomato and the Microbial Community Structure of Rhizosphere Soil Under Salt Stress

SHAO MeiQi^1,²(),ZHAO WeiSong²,SU ZhenHe²,DONG LiHong²,GUO QingGang^2,^*(),MA Ping²

¹College of Plant Protection, Hebei Agricultural University, Baoding 071001, Hebei
²Plant Protection Institute of Hebei Academy of Agricultural and Forestry Sciences/IPM Centre of Hebei Province/Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, Baoding 071000, Hebei

Received:2021-03-17 Accepted:2021-03-31 Online:2021-11-01 Published:2021-11-09
Contact: QingGang GUO

摘要/Abstract

摘要：

【目的】探讨盐胁迫下生防枯草芽孢杆菌（Bacillus subtilis）NCD-2菌株对番茄的促生效果及对土壤微生物群落多样性的影响,为拓宽NCD-2菌株的应用提供理论依据。【方法】采用温室盆栽试验,测定盐胁迫下NCD-2菌株对番茄株高、地上部、根部干重和鲜重的影响,并测定抗逆相关酶超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性和脱落酸（ABA）含量;采用高通量测序（Illumina MiSeq）技术测定NCD-2菌株菌悬液处理（NCD0）、100 mmol·L^-1 NaCl处理（CK100）、NCD-2菌株菌悬液+100 mmol·L^-1 NaCl处理（NCD100）和清水处理作为对照（CK0）条件下土壤真菌、细菌群落结构,分析盐胁迫下NCD-2菌株对番茄根际土壤微生物群落结构的影响。【结果】正常条件下,经NCD-2菌株处理,番茄株高、地上部鲜重、地上部干重、根部鲜重和根部干重分别较对照增加了9.08%、10.37%、16.64%、15.42%和16.78%;在100 mmol·L^-1 NaCl盐胁迫下,经NCD-2菌株处理,番茄株高、地上部鲜重、地上部干重、根部鲜重和根部干重分别较对照增加了16.86%、18.96%、21.32%、10.50%和23.99%。盐胁迫下,经NCD-2菌株处理,番茄体内SOD、POD、CAT活性和ABA含量分别较对照提高了50.45%、56.18%、29.55%和34.60%。细菌群落组成分析表明,在没有盐胁迫的条件下,NCD-2菌株处理后番茄根际放线菌门（Actinobacteria）、酸杆菌门（Acidobacteria）和绿弯菌门（Chloroflexi）菌群的相对丰度分别较清水对照（CK0）提高了7.28%、15.14%和23.03%;节杆菌属（Arthrobacter）、鞘氨醇单胞菌属（Sphingomonas）、微枝形杆菌属（Microvirga）和链霉菌属（Streptomyces）菌群的相对丰度分别较CK0提高了50.88%、15.31%、11.32%和16.41%。在100 mmol·L^-1 NaCl盐胁迫下,经NCD-2菌株处理,番茄根际变形菌门（Proteobacteria）、放线菌门、厚壁菌门（Firmicutes）和芽单胞菌门（Gemmatimonadetes）菌群的相对丰度分别较单独NaCl处理（CK100）提高了6.08%、8.19%、14.11%和4.70%;节杆菌属、芽孢杆菌属（Bacillus）、鞘氨醇单胞菌属和微枝形杆菌属菌群的相对丰度分别较CK100提高了5.54%、31.80%、23.39%和23.08%。真菌群落组成分析表明,在没有盐胁迫的条件下,NCD-2菌株处理后番茄根际被孢菌门（Mortierellomycota）、球囊菌门（Glomeromycota）和壶菌门（Chytridiomycota）菌群相对丰度分别提高至CK0的186%、477%和1 650%;小被孢霉属（Mortierella）、木霉属（Trichoderma）和光黑壳属（Preussia）菌群分别提高至CK0的186%、108%和120%。在100 mmol·L^-1 NaCl盐胁迫下,NCD-2菌株处理后番茄根际被孢菌门、球囊菌门和壶菌门菌群分别提高至CK100的345%、154%和921%;小被孢霉属较CK100提高了246%。【结论】盐胁迫环境下NCD-2菌株处理后通过提高番茄体内抗逆相关酶的活性、ABA的含量,增加番茄根际有益微生物的种群数量,从而提高了番茄对盐胁迫的耐受能力,显著促进了番茄的生长发育。

关键词: 枯草芽孢杆菌, 盐胁迫, 抗氧化酶活性, 高通量测序, 土壤微生物群落结构

Abstract:

【Objective】The objective of this study is to evaluate the growth promotion effect of Bacillus subtilis strain NCD-2 on tomato seedlings under salt stress, as well as the effect of strain NCD-2 on soil microbial community diversity. The results will be useful for expanding the application of strain NCD-2 in agricultural system.【Method】The pot experiments were conducted to evaluate the effects of strain NCD-2 treatment on stem length, aboveground fresh weight, aboveground dry weight, root fresh weight and root dry weight. The activity of resistance-related enzymes such as peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) and the content of abscisic acid (ABA) were measured. High-throughput sequencing (Illumina MiSeq) technique was used to determine the bacterial and fungal community structures in the rhizosphere soil. The four treatments were set as NCD-2 strain suspension treatment (NCD0), 100 mmol·L ^-1 NaCl treatment (CK100), NCD-2 strain suspension + 100 mmol·L^-1 NaCl treatment (NCD100) and water treatment as control (CK0).【Result】Under normal conditions, strain NCD-2 treatment significantly increased the biomass of tomato. The plant height, aboveground fresh weight, aboveground dry weight, root fresh weight and root dry weight were increased by 9.08%, 10.37%, 16.64%, 15.42% and 16.78%, respectively, when compared with the control. Under salt stress, compared to the control, the plant height, aboveground fresh weight, aboveground dry weight, root fresh weight and root dry weight were increased by 16.86%, 18.96%, 21.32%, 10.50% and 23.99% after treated with strain NCD-2, respectively. The activity of resistance-related enzymes SOD, POD, CAT and the content of ABA were increased by 50.45%, 56.18%, 29.55% and 34.60% after treated with strain NCD-2, respectively, when compared with the control. For bacteria community composition analysis, compared to CK0, the relative abundance of bacteria phylum Actinobacteria, Acidobacteria and Chloroflexi was increased by 7.28%, 15.14% and 23.03% after treated with strain NCD-2 without salt stress, respectively. The relative abundance of bacteria genus Arthrobacter, Sphingomonas, Microvirga and Streptomyces was increased by 50.88%, 15.31%, 11.32% and 16.41% after treated with strain NCD-2, respectively. Under 100 mmol·L ^-1 NaCl stress, compared to CK100, the relative abundance of bacteria phylum Proteobacteria, Actinobacteria, Firmicutes and Gemmatimonadetes was increased by 6.08%, 8.19%, 14.11% and 4.70% after treated with strain NCD-2, respectively. The relative abundance of bacteria genus Arthrobacter, Bacillus, Sphingomonas and Microvirga was increased by 5.54%, 31.80%, 23.39% and 23.08% after treated with strain NCD-2, respectively. For fungal community composition analysis, compared to CK0, the relative abundance of fungal phylum Mortierellomycota, Glomeromycota and Chytridiomycota was increased to 186%, 477% and 1 650% of CK0, respectively. The relative abundance of fungal genus Mortierella, Trichoderma and Preussia was increased to 186%, 108%, and 120% of CK0 after treated with strain NCD-2 without salt stress, respectively. Under 100 mmol·L ^-1 NaCl stress, compared to CK100, the relative abundance of fungal phylum Mortierellomycota, Glomeromycota and Chytridiomycota was increased to 345%, 154%, 921% of CK100 after treated with strain NCD-2, respectively. The relative abundance of fungal genus Mortierella was increased by 246% after treated with strain NCD-2.【Conclusion】After the treatment of strain NCD-2 under salt stress, the activity of stress-resistant enzymes and the content of ABA in tomato were increased, and the population of beneficial microorganisms in tomato rhizosphere was increased, thus improving the tolerance of tomato to salt stress and significantly increasing the growth and development of tomato.

Key words: Bacillus subtilis, salt stress, activity of antioxidant enzymes, high-throughput sequencing, soil microbial community structure

邵美琪,赵卫松,苏振贺,董丽红,郭庆港,马平. 盐胁迫下枯草芽孢杆菌NCD-2对番茄促生作用及对土壤微生物群落结构的影响[J]. 中国农业科学, 2021, 54(21): 4573-4584.

SHAO MeiQi,ZHAO WeiSong,SU ZhenHe,DONG LiHong,GUO QingGang,MA Ping. Effect of Bacillus subtilis NCD-2 on the Growth of Tomato and the Microbial Community Structure of Rhizosphere Soil Under Salt Stress[J]. Scientia Agricultura Sinica, 2021, 54(21): 4573-4584.

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盐胁迫下枯草芽孢杆菌NCD-2对番茄促生作用及对土壤微生物群落结构的影响

Effect of Bacillus subtilis NCD-2 on the Growth of Tomato and the Microbial Community Structure of Rhizosphere Soil Under Salt Stress

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