中国农业科学 ›› 2020, Vol. 53 ›› Issue (9): 1860-1873.doi: 10.3864/j.issn.0578-1752.2020.09.013
庄姗1,林伟1,丁军军1,郑欠1,寇馨月1,李巧珍1,李玉中1,2()
收稿日期:
2019-08-02
接受日期:
2019-11-06
出版日期:
2020-05-01
发布日期:
2020-05-13
通讯作者:
李玉中
作者简介:
庄姗,Tel:18519190629;E-mail:489300802@qq.com。
基金资助:
Shan ZHUANG1,Wei LIN1,JunJun DING1,Qian ZHENG1,XinYue KOU1,QiaoZhen LI1,YuZhong LI1,2()
Received:
2019-08-02
Accepted:
2019-11-06
Online:
2020-05-01
Published:
2020-05-13
Contact:
YuZhong LI
摘要:
【目的】探究植物根系分泌的主要组分(有机酸、氨基酸、糖类)对土壤N2O排放及其微生物过程的影响,为选择适宜的植物进而控制土壤N2O排放提供支撑。【方法】通过室内试验分别添加草酸、丝氨酸、葡萄糖于土壤中模拟根系的3种主要分泌物,每种分泌物设置两个浓度水平:低浓度(150 μg C·d -1)和高浓度(300 μg C·d -1),另设置添加蒸馏水的对照组,共7个处理。将土壤置于120 mL玻璃瓶中进行培养,24 h内采集气体样品7次,每次培养2 h,获取N2O排放速率、日累积排放量和同位素特征值(δ 15N bulk、δ 18O和SP(site preference,SP=δ 15N α-δ 15N β))。【结果】添加3种根系分泌物组分后,土壤N2O排放速率均逐渐升高,且均高于对照。高浓度处理组N2O累积排放量为:葡萄糖((3.2±1.3)mg·kg -1·d -1)处理>丝氨酸((2.6±0.5)mg·kg -1·d -1)处理>草酸((1.4±0.2)mg·kg -1·d -1)处理,低浓度处理组为:草酸((2.7±1.3)mg·kg -1·d -1)处理>丝氨酸((1.8±0.4)mg·kg -1·d -1)处理>葡萄糖((1.6±0.8)mg·kg -1·d -1)处理;添加根系分泌物的不同处理间土壤N2O的δ 18O值无明显差异,并稳定在24.1‰—25.6‰,且均显著高于对照((20.1±1.5)‰);土壤N2O的δ 15N bulk值与添加根系分泌物的种类有关,其中草酸处理组为(-20.06±2.22)‰、丝氨酸处理组为(-22.33±1.10)‰、葡萄糖处理组为(-13.86±1.11)‰、对照组为(-23.14±3.72)‰。各处理土壤N2O的SP值的变化范围为13.13‰—15.03‰,根系分泌物浓度越高,SP值越低。综合分析不同处理4个指标(N2O排放速率、N2O的δ 15N bulk、δ 18O和SP值)的不同时刻的检测值与日均值的校正系数,添加根系分泌物后第16小时各处理4个指标的校正系数最接近于1。【结论】在NH+ 4-300 mg N·kg -1的土壤环境下根系分泌物促进N2O的排放,且在培养期间(24 h)土壤N2O排放速率逐渐升高。高浓度处理组葡萄糖对土壤N2O排放速率促进效果最强,低浓度处理组草酸对土壤N2O排放速率促进效果最强。与对照组相比,根系分泌物的添加使N2O的δ 18O值显著升高;与对照组相比,葡萄糖的添加使δ 15N bulk值显著升高。根系分泌物浓度越高,反硝化作用对N2O的贡献越大。
庄姗,林伟,丁军军,郑欠,寇馨月,李巧珍,李玉中. 不同根系分泌物对土壤N2O排放及同位素特征值的影响[J]. 中国农业科学, 2020, 53(9): 1860-1873.
Shan ZHUANG,Wei LIN,JunJun DING,Qian ZHENG,XinYue KOU,QiaoZhen LI,YuZhong LI. Effects of Different Root Exudates on Soil N2O Emissions and Isotopic Signature[J]. Scientia Agricultura Sinica, 2020, 53(9): 1860-1873.
Table 1
Cumulative emissions of N2O and daily weighted average of isotope signature"
处理 Treatment | N2O累计排放量 (mg·kg-1·d-1) | δ18O (‰) | δ15Nbulk (‰) | SP (‰) | |
---|---|---|---|---|---|
草酸 Oxalic acid | CL | 2.68±1.34ab | 24.16±1.55a | -19.82±7.41abc | 17.03±0.40a |
CH | 1.38±0.24bc | 24.08±1.22a | -20.31±5.82abc | 13.13±2.70b | |
丝氨酸 Serine | AL | 1.81±0.45abc | 25.33±0.39a | -24.44±1.95c | 15.71±0.78ab |
AH | 2.56±0.45ab | 24.68±1.11a | -20.21±2.11abc | 14.35±1.47ab | |
葡萄糖 Glucose | PL | 1.61±0.79bc | 25.59±0.25a | -13.12±1.13a | 16.16±1.40a |
PH | 3.23±1.33a | 24.09±2.30a | -14.6±4.38ab | 15.18±1.04ab | |
对照 Control treatment | 0.90±0.09c | 20.06±1.52b | -23.14±3.72c | 13.29±1.40b | |
添加根系分泌物 Adding root exudates | <0.001** | 0.196 | 0.089 | 0.032* | |
组分 Component | 0.718 | 0.495 | 0.017* | 0.718 | |
浓度 Concentration | 0.38 | 0.261 | 0.726 | 0.012* | |
组分×浓度 Component×Concentration | 0.0278* | 0.611 | 0.51 | 0.222 |
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