代乳粉添加甘露寡糖对7—28日龄湖羊羔羊胃肠道发育的影响

doi:10.3864/j.issn.0578-1752.2020.02.014

中国农业科学 ›› 2020, Vol. 53 ›› Issue (2): 398-408.doi: 10.3864/j.issn.0578-1752.2020.02.014

所属专题：专题——幼龄反刍动物培养

• 专题：幼龄反刍动物培育研究 • 上一篇下一篇

代乳粉添加甘露寡糖对7—28日龄湖羊羔羊胃肠道发育的影响

郑琛¹,李发弟^2,³,李飞²,周巨旺¹,段鹏伟¹,刘绘汇¹,樊海苗¹,朱威力¹,刘婷¹()

1 甘肃农业大学动物科学技术学院,兰州 730070
2 兰州大学草地农业科技学院,草地农业生态系统国家重点实验室/ 农业农村部草牧业创新重点实验室,兰州 730020
3 甘肃省肉羊繁育生物技术工程实验室,甘肃民勤 733300

收稿日期:2019-06-24 接受日期:2019-08-14 出版日期:2020-01-16 发布日期:2020-02-17
联系方式: 郑琛,E-mail：zhengc@gsau.edu.cn。
基金资助:
国家自然科学基金(31560646);国家自然科学基金(31860657)

Effects of Adding Mannan Oligosaccharides to Milk Replacer on the Development of Gastrointestinal Tract of 7-28 Days Old Hu Lambs

ZHENG Chen¹,LI FaDi^2,³,LI Fei²,ZHOU JuWang¹,DUAN PengWei¹,LIU HuiHui¹,FAN HaiMiao¹,ZHU WeiLi¹,LIU Ting¹()

1 College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070
2 State Key Laboratory of Grassland Agro-ecosystems, Key laboratory of Grassland Livestock Industry Innovation/Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020
3 Engineering Laboratory of Mutton Sheep Breeding and Reproduction Biotechnology in Gansu Province, Minqin 733300, Gansu

Received:2019-06-24 Accepted:2019-08-14 Published:2020-01-16 Online:2020-02-17

摘要/Abstract

摘要：

【目的】探讨代乳粉中添加甘露寡糖（mannan oligosaccharides,MOS）对7—28日龄湖羊羔羊胃肠道生长发育的影响。【方法】选择同质性良好的7日龄湖羊公羔（双羔）30只,随机分为2组,每组15只,每只为1个重复,对照组羔羊饲喂不含MOS的代乳粉,试验组羔羊饲喂含0.2 % MOS的代乳粉,试验期21d。羔羊28日龄时,两个试验组各随机选择8只羔羊屠宰,取出消化道,称量各胃室和肠段包含内容物的质量和净质量,量取各肠段长度,用以计算各部位的相对质量和内容物分布,以及各肠段的相对长度。多聚甲醛固定皱胃胃底腺区及十二指肠、空肠和回肠中段的组织样品,测定组织形态和小肠上皮细胞凋亡率。采集十二指肠、空肠和回肠的黏膜样品,测定紧密连接蛋白1 (claudin 1)、闭锁小带1（zonula occludens-1,ZO-1）和闭锁蛋白（occludin）的mRNA表达量。【结果】除空肠相对长度外（%全肠长度,P=0.040）,MOS对羔羊胃肠指数（%活体质量）、胃肠相对质量（%全胃质量、%全肠质量和%全胃肠质量）、肠道相对长度（%全肠长度）、内容物相对活体质量（%活体质量）、胃肠内容物相对总胃/肠内容物及总胃肠内容物相对质量（%总胃内容物、总肠内容物、总胃肠内容物）、小肠上皮细胞凋亡率和小肠黏膜claudin 1蛋白mRNA的表达量均没有产生显著影响（P＞0.05）,但MOS显著提高羔羊十二指肠绒毛高度和肌层厚度并显著降低绒毛宽度（P=0.033,P=0.047,P=0.015）,显著上调空肠ZO-1蛋白mRNA表达量（P=0.028）,此外,MOS有提高羔羊回肠绒毛高度、绒毛宽度和隐窝深度、皱胃肌层厚度及回肠occludin蛋白mRNA表达量的趋势（P=0.075,P=0.078,P=0.085,P=0.084,P=0.052）。【结论】MOS对7—28日龄湖羊羔羊胃肠道相对质量、长度和内容物分布基本无显著影响,但可改善十二指肠和回肠绒毛及肌层的组织形态,维持小肠屏障功能,有利于提高养分消化率。

关键词: 羔羊, 甘露寡糖, 代乳粉, 胃肠道, 发育

Abstract:

【Objective】This study was conducted to investigate the effects of mannan oligosaccharides (MOS) supplementation to milk replacer on the development of gastrointestinal tract of 7-28 day-old Hu lambs. 【Method】 Thirty 7 day-old Hu male lambs were chosen and divided into 2 groups randomly, fifteen lambs in each group and each lamb as a repeat. Lambs were fed milk replacer with or without 0.2 % MOS, respectively. The test lasted for 21 days. Eight lambs were selected from each group randomly and slaughtered at 28 day-old. The weights of the compound stomach and the intestinal tract with and without content, and lengths of the intestinal tract were measured, and the relative quality and length were calculated. While the tissue samples from fundus gland region of the abomasum, the middle part of duodenum, jejunum and ileum were fixed in paraformaldehyde to analyse the histomorphology, and the apoptotic rate of intestinal epithelial cells as well. And the mRNA expression of claudin 1, zonula occludens-1 (ZO-1), and occludin protein of duodenum, jejunum and ileum mucosa were measured.【Result】The results showed that except relative length of jejunum (P=0.040), the relative weights (% body weight, % stomach weight, % intestinal tract weight, and % gastrointestinal tract weight), relative lengths (% intestinal tract length), content of stomach and intestinal tract (% body weight, % stomach content weight, % intestinal tract content weight, and % gastrointestinal tract content weight), the apoptotic rate of intestinal epithelial cells and mRNA expression of claudin 1 protein in intestinal tract of lambs were not affected by MOS (P＞0.05). However, MOS elongated the villus height and the muscular thickness, and decreased the villus width of lamb duodenum significantly (P=0.033, P=0.047, P=0.015). MOS also up-regulated the mRNA expression of ZO-1 protein of lamb jejunum significantly (P=0.028). And there was a tendency that MOS elongated villus height, width and crypt depth of ileum, muscular thickness of abomasum, and mRNA expression of occludin protein of ileum (P=0.075, P=0.078, P=0.085, P=0.084, P=0.052).【Conclusion】MOS almost did not affect the relative weights, lengths, and content distribution of gastrointestinal tract of 7-28 days old Hu lambs, but improved the histomorphology of duodenum and ileum, indicating it could maintain barrier function of intestinal tract and benefit to nutrients digestibility.

Key words: lamb, mannan oligosaccharides, milk replacer, gastrointestinal tract, development

郑琛,李发弟,李飞,周巨旺,段鹏伟,刘绘汇,樊海苗,朱威力,刘婷. 代乳粉添加甘露寡糖对7—28日龄湖羊羔羊胃肠道发育的影响[J]. 中国农业科学, 2020, 53(2): 398-408.

ZHENG Chen,LI FaDi,LI Fei,ZHOU JuWang,DUAN PengWei,LIU HuiHui,FAN HaiMiao,ZHU WeiLi,LIU Ting. Effects of Adding Mannan Oligosaccharides to Milk Replacer on the Development of Gastrointestinal Tract of 7-28 Days Old Hu Lambs[J]. Scientia Agricultura Sinica, 2020, 53(2): 398-408.

图/表 8

表1

表2

表3

MOS对羔羊胃肠相对质量的影响"

项目Item		对照组Control	MOS组 MOS	SEM	P值 P value
胃室 Stomach
瘤胃 Rumen	%全胃质量 % the quality of total stomach	29.70	30.45	0.58	0.533
瘤胃 Rumen	%全胃肠质量 % the quality of total gastrointestinal tract	6.71	6.62	0.27	0.869
网胃 Reticulum	%全胃质量 % the quality of total stomach	9.16	9.46	0.40	0.724
网胃 Reticulum	%全胃肠质量 % the quality of total gastrointestinal tract	2.05	2.06	0.10	0.939
瓣胃 Omasum	%全胃质量 % the quality of total stomach	4.08	4.15	0.21	0.883
瓣胃 Omasum	%全胃肠质量 % the quality of total gastrointestinal tract	0.92	0.89	0.05	0.795
皱胃 Abomasum	%全胃质量 % the quality of total stomach	57.05	55.94	0.74	0.471
皱胃 Abomasum	%全胃肠质量 % the quality of total gastrointestinal tract	12.81	12.14	0.35	0.356
肠道 Intestinal tract
十二指肠 Duodenum	%全肠质量 % the quality of total intestinal tract	3.22	2.93	0.14	0.297
十二指肠 Duodenum	%全胃肠质量 % the quality of total gastrointestinal tract	2.49	2.28	0.09	0.292
空肠Jejunum	%全肠质量 % the quality of total intestinal tract	64.21	61.69	1.07	0.254
空肠Jejunum	%全胃肠质量 % the quality of total gastrointestinal tract	49.80	48.36	1.06	0.517
回肠 Ileum	%全肠质量 % the quality of total intestinal tract	1.91	2.41	0.21	0.258
回肠 Ileum	%全胃肠质量 % the quality of total gastrointestinal tract	1.69	1.88	0.13	0.477
盲肠 Cecum	%全肠质量 % the quality of total intestinal tract	5.57	6.89	0.44	0.135
盲肠 Cecum	%全胃肠质量 % the quality of total gastrointestinal tract	4.32	5.38	0.34	0.116
结肠 Colon	%全肠质量 % the quality of total intestinal tract	18.95	19.43	0.57	0.686
结肠 Colon	%全胃肠质量 % the quality of total gastrointestinal tract	14.67	15.17	0.41	0.558
直肠 Rectum	%全肠质量 % the quality of total intestinal tract	6.14	6.65	0.23	0.275
直肠 Rectum	%全胃肠质量 % the quality of total gastrointestinal tract	4.75	5.19	0.16	0.162

表3

表4

表5

表6

MOS对羔羊胃肠内容物相对总胃/肠内容物及总胃肠内容物相对质量的影响"

项目 Item		对照组Control	MOS组 MOS	SEM	P值 P value
胃室内容物 Stomach content
瘤胃内容物 Rumen content	%全胃内容物质量 % the quality of total stomach content	26.96	33.15	2.56	0.239
瘤胃内容物 Rumen content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	17.30	21.12	1.80	0.306
网胃内容物 Reticulum content	%全胃内容物质量 % the quality of total stomach content	4.99	6.68	1.28	0.532
网胃内容物 Reticulum content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	3.50	4.06	0.03	0.742
瓣胃内容物 Omasum content	%全胃内容物质量 % the quality of total stomach content	0.23	0.24	0.03	0.988
瓣胃内容物 Omasum content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	0.14	0.13	0.01	0.698
皱胃内容物 Abomasum content	%全胃内容物质量 % the quality of total stomach content	68.58	60.09	2.71	0.119
皱胃内容物 Abomasum content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	43.14	39.30	2.35	0.434
肠道内容物 Intestinal tract content
十二指肠内容物 Duodenum content	%全肠内容物质量 % the quality of total intestinal tract content	7.20	6.23	1.63	0.777
十二指肠内容物 Duodenum content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	1.98	2.22	0.39	0.765
空肠内容物 Jejunum content	%全肠内容物质量 % the quality of total intestinal tract content	39.34	46.21	2.22	0.126
空肠内容物 Jejunum content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	14.28	16.95	1.69	0.451
回肠内容物 Ileum content	%全肠内容物质量 % the quality of total intestinal tract content	1.75	1.45	0.31	0.666
回肠内容物 Ileum content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	0.80	0.57	0.20	0.618
盲肠内容物 Cecum content	%全肠内容物质量 % the quality of total intestinal tract content	12.73	9.88	1.35	0.312
盲肠内容物 Cecum content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	4.88	3.51	0.70	0.345
结肠内容物 Colon content	%全肠内容物质量 % the quality of total intestinal tract content	33.02	30.57	2.83	0.681
结肠内容物 Colon content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	12.49	10.25	1.17	0.356
直肠内容物 Rectum content	%全肠内容物质量 % the quality of total intestinal tract content	5.99	7.80	1.05	0.405
直肠内容物 Rectum content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	2.06	2.77	0.42	0.414

表6

表7

表8

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营养成分 Ingredients^1}	含量 Concentration
干物质 Dry matter	95.68
蛋白质 Protein	24.89
粗脂肪 Ether extract	17.50
粗纤维 Crude fiber	2.16
粗灰分 Crude ash	5.81
钙 Calcium	1.02
总磷 Total phosphorus	0.59

项目Item	对照组Control	MOS组 MOS	SEM	P值 P value
皱胃 Abomasum
黏膜厚度 Mucosa thickness	333.36	331.46	9.74	0.927
肌层厚度 Muscular thickness	548.48	686.99	40.11	0.084
十二指肠 Duodenum
绒毛高度 Villus height	361.99b	446.39a	20.44	0.033
绒毛宽度 Villus width	80.04a	61.96b	3.92	0.015
隐窝深度 Crypt depth	148.87	163.33	8.46	0.414
肌层厚度 Muscular thickness	161.93b	201.87a	10.25	0.047
绒毛高度/隐窝深度值V/C	2.57	2.82	0.11	0.258
空肠Jejunum
绒毛高度 Villus height	431.59	445.30	20.29	0.748
绒毛宽度 Villus width	71.13	68.01	2.04	0.464
隐窝深度 Crypt depth	173.48	158.21	6.65	0.265
肌层厚度 Muscular thickness	157.73	153.01	8.27	0.786
绒毛高度/隐窝深度值V/C	2.59	2.89	0.09	0.118
回肠 Ileum
绒毛高度 Villus height	373.81	428.38	15.25	0.075
绒毛宽度 Villus width	68.91	78.87	2.81	0.078
隐窝深度 Crypt depth	176.96	213.10	10.38	0.085
肌层厚度 Muscular thickness	274.31	230.82	18.54	0.267
绒毛高度/隐窝深度值V/C	2.19	2.15	0.06	0.775

项目Item	对照组Control	MOS组 MOS	SEM	P值 P value
十二指肠 Duodenum
Claudin 1	0.98	0.94	0.10	0.863
ZO-1	0.92	1.05	0.08	0.403
Occludin	0.86	1.18	0.10	0.112
细胞凋亡 Apoptosis (%)	11.27	5.04	2.90	0.302
空肠Jejunum
Claudin 1	0.91	1.09	0.21	0.671
ZO-1	0.80b	1.20a	0.10	0.028
Occludin	0.96	1.04	0.08	0.630
细胞凋亡 Apoptosis (%)	21.09	18.08	5.40	0.791
回肠 Ileum
Claudin 1	0.87	0.81	0.18	0.875
ZO-1	0.92	1.06	0.07	0.362
Occludin	0.83	1.17	0.09	0.052
细胞凋亡 Apoptosis (%)	47.29	37.62	6.54	0.487

代乳粉添加甘露寡糖对7—28日龄湖羊羔羊胃肠道发育的影响

Effects of Adding Mannan Oligosaccharides to Milk Replacer on the Development of Gastrointestinal Tract of 7-28 Days Old Hu Lambs

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项目Item	对照组Control	MOS组 MOS	SEM	P值 P value
胃室 Stomach
瘤胃 Rumen	0.54	0.50	0.02	0.386
网胃 Reticulum	0.16	0.15	0.01	0.555
瓣胃 Omasum	0.08	0.07	0.01	0.488
皱胃 Abomasum	1.04	0.92	0.04	0.138
肠道 Intestinal tract
十二指肠 Duodenum	0.20	0.17	0.01	0.066
空肠Jejunum	4.00	3.71	0.14	0.316
回肠 Ileum	0.13	0.14	0.01	0.697
盲肠 Cecum	0.35	0.41	0.02	0.201
结肠 Colon	1.18	1.15	0.04	0.675
直肠 Rectum	0.38	0.39	0.01	0.663

项目Item	对照组Control	MOS组 MOS	SEM	P值 P value
十二指肠 Duodenum	2.29	2.01	0.09	0.107
空肠Jejunum	81.84a	80.53b	0.33	0.040
回肠 Ileum	0.83	0.95	0.01	0.292
盲肠 Cecum	0.91	1.12	0.06	0.094
结肠 Colon	11.96	12.89	0.29	0.115
直肠 Rectum	2.17	2.49	0.11	0.151

项目Item	对照组Control	MOS组 MOS	SEM	P值 P value
胃室内容物 Stomach content
瘤胃内容物 Rumen content	1.44	1.67	0.20	0.587
网胃内容物 Reticulum content	0.24	0.34	0.07	0.473
瓣胃内容物 Omasum content	0.01	0.01	0.001	0.946
皱胃内容物 Abomasum content	3.43	3.18	0.24	0.623
肠道内容物 Intestinal tract content
十二指肠内容物 Duodenum content	0.15	0.14	0.02	0.868
空肠内容物Jejunum content	1.26	1.35	0.20	0.833
回肠内容物 Ileum content	0.11	0.05	0.03	0.378
盲肠内容物 Cecum content	0.43	0.30	0.08	0.422
结肠内容物 Colon content	1.08	0.81	0.13	0.298
直肠内容物 Rectum content	0.17	0.21	0.03	0.503

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