父源性印记基因在牛早期胚胎中的表达

doi:10.3864/j.issn.0578-1752.2013.18.019

摘要/Abstract

摘要： 【目的】研究父源印记基因在牛早期胚胎中的表达模式。【方法】利用Real-time PCR技术，检测Gnas、Grb10和Xist这3个父源性印记基因在牛体外受精（In vitro Fertilization，IVF）和孤雌激活（Parthenogenetic Activation，PA）胚胎各发育阶段的表达情况。【结果】对于IVF和PA两种来源的牛胚胎，Gnas在胚胎各发育阶段的表达没有明显差异；Grb10在2细胞阶段的PA胚胎中的表达量明显高于在IVF胚胎的，但从4细胞阶段到囊胚阶段，Grb10在两种来源的牛胚胎中表达没有明显差异；从2细胞阶段到8细胞阶段，Xist在两种来源的牛胚胎中表达没有明显差异，但从16细胞期开始，Xist在PA胚胎中的表达明显高于IVF胚胎的表达量。【结论】对于牛IVF胚胎和PA胚胎，Gnas、Grb10和Xist从2细胞期到囊胚期都可以检测到表达，但表达模式不同。这3个父源性印记基因在牛胚胎发育过程中可能发挥着重要的作用。

关键词: 牛 , 父源性印记基因 , 早期胚胎 , 表达

Abstract: 【Objective】The objective of the experiment is to investigate the expression pattern of paternal imprinting genes in bovine early stage embryo.【Method】The expression profile of paternal imprinting genes Gnas, Grb10, and Xist were detected in bovine embryo derived from in vitro fertilization (IVF) and parthenogenetic activation (PA) by real-time PCR. 【Result】 There were no difference in imprinted expression profile of Gnas between IVF and PA embryo. Grb10 expression was significantly higher during 2-cell stage in PA embryo than that of IVF embryo, however, no difference was observed in expression of Grb10 between IVF and PA embryos from 4-cell satge to blastcyst stage. Xist expression was similar between PA and IVF embryo from 2-cell to 8-cell stage. But in the later stage, Xist expression was higher significantly in PA embryo than that in IVF embryo. 【Conclusion】Gnas, Grb10 and Xist were expressed differently in bovine IVF embryo and PA embryo from 2-cell stage to the blastocyst stage, and they might play an important role in bovine embryo development.

Key words: bovine , paternal imprinted genes , early stage embryo , expression

张春强, 赵德超, 韩志玲, 张文广, 张家新. 父源性印记基因在牛早期胚胎中的表达[J]. 中国农业科学, 2013, 46(18): 3887-3893.

ZHANG Chun-Qiang, ZHAO De-Chao, HAN Zhi-Ling, ZHANG Wen-Guang, ZHANG Jia-Xin. The Expression of Paternal Imprinting Genes in Bovine Early Stage Embryo[J]. Scientia Agricultura Sinica, 2013, 46(18): 3887-3893.

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