中国农业科学 ›› 2013, Vol. 46 ›› Issue (9): 1946-1955.doi: 10.3864/j.issn.0578-1752.2013.09.023

• 研究简报 • 上一篇    下一篇

陆地棉trihelix转录因子基因响应非生物胁迫表达谱分析

 李月, 孙杰, 谢宗铭, 李全胜, 司爱君, 陈受宜   

  1. 1.石河子大学农学院/新疆兵团绿洲生态农业重点实验室,新疆石河子 832003
    2.新疆农垦科学院分子农业技术育种中心/作物种质创新与基因资源利用兵团重点实验室,新疆石河子 832000
    3.中国科学院遗传与发育生物学研究所/国家植物基因组重点实验室,北京 100101
  • 收稿日期:2012-12-04 出版日期:2013-05-01 发布日期:2013-03-28
  • 通讯作者: 通信作者谢宗铭,Tel:0993-6683583;E-mail:xiezm2008@yahoo.com.cn;通信作者陈受宜,Tel:010-64886859;E-mail:sychen@genetics.ac.cn
  • 作者简介:李月,Tel:15010505040;E-mail:liyue6905@126.com
  • 基金资助:

    转基因生物新品种培育科技重大专项(2009ZX08009-090B)、兵团博士资金项目(2008JC03)

Expression Profiling of Cotton (Gossypium hirsutum L.) Trihelix Genes Responsive to Abiotic Stresses

 LI  Yue, SUN  Jie, XIE  Zong-Ming, LI  Quan-Sheng, SI  Ai-Jun, CHEN  Shou-Yi   

  1. 1.Agricultural College, Shihezi University/The Key Oasis Eco-Agriculture Laboratory of Xinjiang Production and Construction Group, Shihezi 832003, Xinjiang
    2.Center for Molecular Agrobiotechnology and Breeding, Xinjiang Academy of Agricultural and Reclamation Science/Xinjiang Production and Construction Group Key Laboratory of Crop Germplasm Enhancement and Gene Resources Utilization, Shihezi 832000, Xinjiang
    3.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences/National Key Laboratory of Plant Genomics, Beijing 100101
  • Received:2012-12-04 Online:2013-05-01 Published:2013-03-28

摘要: 【目的】对陆地棉trihelix转录因子基因在多种胁迫下的应答表达模式进行研究分析,为棉花抗逆相关基因克隆及阐明棉花抗逆分子机制奠定基础。【方法】根据已知24个trihelix转录因子EST序列设计引物,通过RT-PCR和qRT-PCR的方法分析棉花(Gossypium hirsutum L.)GhGT在高盐(200 mmol.L-1NaCl)、干旱、低温(4℃)等非生物胁迫和外源激素ABA(100 μmol.L-1)处理下的表达模式。【结果】12个基因响应高盐和干旱胁迫应答,7个基因响应冷胁迫应答,应答ABA处理的基因9个。此外,有3个基因(GhGT2、GhGT18和GhGT23)在4种处理下均有应答。【结论】GhGTs在陆地棉的非生物胁迫适应过程中可能具有重要的作用。

关键词: 棉花 , trihelix转录因子 , 非生物胁迫 , 表达分析

Abstract: 【Objective】The objective of this study is to dissect cotton trihelix genes expression alteration responsive to different abiotic stresses, thus providing a foundation for stress-related genes cloning and elucidating the molecular mechanism of cotton stress-resistance.【Method】Semi-quantitative RT-PCR and real-time PCR were employed to reveal the expression profiling of 24 GhGTs under different abiotic stresses (200 mmol.L-1 NaCl, drought, 4℃) and exogenous hormone (100 μmol.L-1ABA) treatments.【Result】The results showed that the expression of 12 GhGT genes were up- and down-regulated by salinity and drought stresses, and that of 7 GhGT genes and 9 GhGT genes were up- and down-regulated by cold and ABA treatments, respectively. Moreover, three genes including GhGT2, GhGT18 and GhGT23 were response to all above treatments. 【Conclusion】The results suggested that GhGTs may play important roles in upland cotton plant abiotic stress adaption.

Key words: cotton , trihelix transcription factor , abiotic stress , expression