妊娠后期胎儿宫内生长受限对出生后羔羊内脏器官的影响

doi:10.3864/j.issn.0578-1752.2012.15.015

摘要/Abstract

摘要： 【目的】研究妊娠后期胎儿宫内生长受限对出生后羔羊内脏器官的影响。【方法】选择健康的蒙古绵羊45只（经同期发情且同期受孕），妊娠（90±1） d分为自由采食组（CG）、0.33 MJ ME•kgw-0.75•d-1（RG1）以及0.175 MJ ME•kgw-0.75•d-1（RG2）3个营养水平处理组，进行不同营养水平的限饲饲养。各组分别于羔羊初生及28周龄时选择4只屠宰，取出内脏。【结果】妊娠后期不同营养水平限饲母羊，严重限制了RG1（P＜0.05）、RG2（P＜0.01）组羔羊的平均初生体重：①初生时，RG1组羔羊仅肺脏重和肺脏组织总DNA含量显著低于CG组（P＜0.05），脾脏重及脾脏组织总DNA含量极显著低于CG组（P＜0.01）；而 RG2组羔羊肺脏和脾脏均显著低于CG组（P＜0.01），心脏、肝脏、肾脏、总胃及小肠重均显著低于CG组（P＜0.05）；其中，心脏和肾脏总DNA含量、肺脏总DNA含量和蛋白与DNA含量比、肝脏组织蛋白和DNA含量比均显著低于CG组（P＜0.05）。②28周龄时，RG1组胎儿期生长受限的肺脏、脾脏重及其总DNA含量与对照组差异不显著（P＞0.05）；除心脏外，RG2组胎儿期生长受限的肝脏、肺脏及脾脏重出生后经过28周的生长与CG组差异不显著（P＞0.05）；RG2组胎儿期降低的肾脏、脾脏细胞总DNA含量，肝脏细胞蛋白和DNA比，在试验结束羔羊28周龄时与CG组差异不显著（P＞0.05），并且肺脏细胞蛋白和DNA比显著高于CG组（P＜0.05），而心脏和肺脏组织总DNA含量仍显著低于CG组（P＜0.05）。【结论】妊娠后期胎儿宫内生长受限通过对其胎儿内脏器官细胞增殖与增肥不同程度的影响而改变了出生后羔羊内脏器官的生长发育轨迹。

关键词: 妊娠后期, 胎儿宫内生长受限, 羔羊, 内脏器官

Abstract: 【Objective】 The effects of intrauterine growth restriction during late pregnancy on the visceral organs of postnatal lambs were studied. 【Method】 Forty-five Mongolia ewes, mated at a synchronized oestrus, were divided into three groups and offered three diets during late pregnancy (90±1) days. The dietary treatments were ad libitum access to feed (control group, CG), 0.33 MJ ME•kgw-0.75•d-1 (restricted group1, RG1), and 0.175 MJ ME•kgw-0.75•d-1 (restricted group 2, RG2), respectively. Four lambs in each group were selected and slaughtered immediately after parturition (neonatal lambs) and at 28-wk of age, and the major visceral organs were removed and weighed separately.【Result】 The results indicated that the maternal undernutrition reduced the average birth weight in RG1 (P＜0.05) and RG2 (P＜0.01) compared to CG. For neonatal lambs, the weights and the DNA content both in the lung (P＜0.05) and spleen (P＜0.01) in RG1 were significantly decreased compared with CG. In RG2, the weights of the lung and spleen were lower (P＜0.01), and the weights of heart, liver, kidney, stomach, small intestine were lower than those in CG (P＜0.05). The DNA content in kidney and heart, the ratio between the DNA content and protein in lung and liver were decreased compared with CG (P＜0.05). At 28 wk of age, the weights of lung and spleen and their DNA contents in RG1 had no significant difference compared with CG (P＞0.05). In RG2, except for heart, there were no significant difference in weights of lung, spleen and liver compared with CG (P＞0.05), and the difference of the DNA content in kidney and spleen, the protein/DNA ratio in liver were not observed (P＞0.05), and the protein/DNA ratio in lung increased compared with CG (P＜0.05). The DNA content in heart and lung, however, was greatly lower (P＜0.05) than those of CG. 【Conclusion】 It was concluded that intrauterine growth restriction during the late pregnancy had an adverse effect on weight and cellularity of postnatal visceral organs, and these perturbations of hyperplasia or hypertrophy in fetal visceral organs development may have a significant potential effect on postnatal growth and adult health.

Key words: late pregnancy, intrauterine growth restriction, postnatal lambs, visceral Organs

高峰, 刘迎春. 妊娠后期胎儿宫内生长受限对出生后羔羊内脏器官的影响[J]. 中国农业科学, 2012, 45(15): 3130-3136.

GAO Feng, LIU Ying-Chun. Effect of Intrauterine Growth Restriction During Late Pregnancy on Visceral Organs of Postnatal Lambs[J]. Scientia Agricultura Sinica, 2012, 45(15): 3130-3136.

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