促孕散对不孕奶牛血清中微量元素和白介素的影响

doi:10.3864/j.issn.0578-1752.2012.13.021

摘要/Abstract

摘要： 【目的】探讨经促孕散对不孕奶牛治疗前后血清中微量元素（Cu、Fe、Mn、Zn）与白介素（IL-1、IL-6）的含量变化规律。【方法】利用火焰法、原子吸收光谱法和酶联免疫吸附法检测实验组和正常组治疗前、后血清中微量元素和白介素的含量。【结果】①促孕散治疗持久，黄体有效率为91.67%，卵巢静止有效率为88.89%，②持久黄体型治疗前血清中Cu、Zn含量显著高于正常组（P＜0.05），Mn含量极显著低于正常组（P＜0.01），治疗后血清中含量接近正常，且含量水平差异不显著（P＞0.05），Fe、IL-1、IL-6含量治疗前后与正常组比较差异不显著，③卵巢静止型治疗前血清中Mn含量低于正常组（P＜0.01），IL-1含量高于正常组（P＜0.05），治疗后逐步与正常组含量水平差异不显著（P＞0.05），Cu、 Fe、Zn、IL-6含量治疗前后与正常组比较差异不显著（P＞0.05）。【结论】促孕散对不孕奶牛血清中部分微量元素和白介素含量有微调作用，在奶牛不孕症治疗效果上有不可忽视的作用。

关键词: 促孕散, 奶牛, 持久黄体, 卵巢静止, 血清, 微量元素, 白介素

Abstract: 【Objective】The law of trace elements (Cu, Fe, Mn, Zn) and interleukin (IL-1, IL-6) contents in serum using fertility-promoting powder to treat infertility caused by ovarian disease of dairy cattle were studied. 【Method】 Flame atomic absorption spectrometry and enzyme-linked immunization absorb assay were used to detect the levels of trace elements and interleukin contents in serum from the experimental and normal groups before and after treatment. 【Result】 After treatment, the effective rate of persistent corpus luteum was 91.67%, the effective rate of ovarian static was 88.89%. In persistent corpus luteum body, Cu, Zn contents were significantly higher than normal group in pre-treatment (P＜0.05) , Mn content was significantly lower than the normal group (P＜0.01), after treatment, their content levels closed to the normal group, and the difference was not significant (P＞0.05). The Fe, IL-1, and IL-6 contents closed to the normal group before and after treatment. In ovarian static body, Mn content was significantly lower than the normal group in pre-treatment (P＜0.01), IL-1 content was higher than the normal group (P＜0.05), after treatment, the contents were not significantly different (P＞0.05), but Cu,Fe,Zn, and IL-6 contents were not significantly different compared with the normal group before and after treatment (P＞0.05). 【Conclusion】The effect of fertility-promoting powder on trace elements and interleukin in serum can not be ignored in treatment of infertility caused by ovarian disease of dairy cattle.

邓敏, 谷新利, 梁光华, 刘贤侠, 高树, 徐雷. 促孕散对不孕奶牛血清中微量元素和白介素的影响[J]. 中国农业科学, 2012, 45(13): 2758-2763.

DENG Min, GU Xin-Li, LIANG Guang-Hua, LIU Xian-Xia, GAO Shu, XU Lei. Effects of Fertility-Promoting Powder on Trace Elements and Interleukin in Serum in Infertile Dairy Cattle[J]. Scientia Agricultura Sinica, 2012, 45(13): 2758-2763.

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