中国农业科学 ›› 2012, Vol. 45 ›› Issue (4): 768-773.doi: 10.3864/j.issn.0578-1752.2012.04.018

• 畜牧·资源昆虫 • 上一篇    下一篇

ESR基因在苏姜猪世代选育中的遗传变异及与猪群繁殖性状的关联

 王宵燕, 何庆玲, 经荣斌, 宋成义   

  1. 1.扬州大学动物科学与技术学院,江苏扬州 225009
  • 收稿日期:2011-10-17 出版日期:2012-02-15 发布日期:2011-12-31
  • 通讯作者: 王宵燕,E-mail:wxy.yzu@gmail.com
  • 作者简介:王宵燕,E-mail:wxy.yzu@gmail.com
  • 基金资助:

    江苏省科技厅高新技术项目(BG2006303)、江苏省研究生培养创新工程(CX09B_316Z)

Genetic Variation of ESR Gene in Different Generations of Sujiang Pigs and the Impact on Reproductive Traits of Pigs

 WANG  Xiao-Yan, HE  Qing-Ling, JING  Rong-Bin, SONG  Cheng-Yi   

  1. 1.扬州大学动物科学与技术学院,江苏扬州 225009
  • Received:2011-10-17 Online:2012-02-15 Published:2011-12-31

摘要: 【目的】探讨苏姜猪ESR基因的PvuⅡ酶切位点多态性在世代选育过程中的遗传变异及与猪群繁殖性状的关系。【方法】采用PCR-RFLP的方法对906头、2—6世代苏姜猪ESR基因PvuⅡ酶切位点的多态性进行分析,并采用最小二乘法和单因子方差分析该位点多态性与苏姜猪群的繁殖性状的关联效应。【结果】ESR基因在苏姜猪的第2—6世代中B等位基因频率呈现出不断增加的趋势,BB基因型个体从第三世代开始出现,并且频率呈现出上升的趋势。仅考虑公猪基因型时,与配母猪总产仔数、产活仔数及死胎数之间无显著差异,但不同基因型公、母猪交配后母猪产仔数则有显著差异(P<0.05)。【结论】ESR基因的PvuⅡ酶切位点的多态性对不同基因型公、母猪交配后母猪的产仔数有显著影响。

关键词: ESR基因, 苏姜猪, 遗传变异, 繁殖性能

Abstract: 【Objective】This study was aimed to analyze PvuⅡ polymorphism of ESR gene in different generations of Sujiang pigs and the correlation between the polymorphism and the reproductive traits of Sujiang pigs. 【Method】 PCR-RFLP was used to diagnose polymorphism of ESR gene in 906 Sujiang pigs of 2-6 generations. Least square method and one-way anova were performed to evaluate the association of this polymorphism site with reproductive traits of pigs. 【Result】 The frequency of allele B of ESR gene in Sujiang pigs of 2-6 generations showed an increasing trend. Pigs with BB genotypes appeared in the third generations and then presented an upward trend in the frequency. There was no significant relationship between the polymorphism of ESR gene and litter size of sows mated to different genotypes of boars. But they had significant impacts on litter size that different genotypes of boars mated with different genotypes of sows (P<0.05). 【Conclusion】 The selection of boars for reproductive traits of Sujiang pig must be taken into consideration.

Key words: ESR gene, Sujiang pig, genetic variation, reproductive traits