不同剂量胰岛素、胰高血糖素和甲状腺素处理对奶牛乳腺上皮细胞FcRn mRNA表达的影响

doi:10.3864/j.issn.0578-1752.2011.20.016

中国农业科学 ›› 2011, Vol. 44 ›› Issue (20): 4279-4283.doi: 10.3864/j.issn.0578-1752.2011.20.016

不同剂量胰岛素、胰高血糖素和甲状腺素处理对奶牛乳腺上皮细胞FcRn mRNA表达的影响

胡小丽, 张春林, 王加启, 赵圣国, 章玉涛, 卜登攀, 周凌云

1.中国农业科学院北京畜牧兽医研究所/动物营养学国家重点实验室

收稿日期:2010-08-30 出版日期:2011-10-15 发布日期:2010-11-26
通讯作者: 通信作者王加启，Tel：010-62815833；Fax；010-62897587；E-mail：wang-jia-qi@263.net
作者简介:胡小丽，Tel：010-62815833；Fax：010-62897587；E-mail：huxiaoli513822@126.com
基金资助:
国家自然科学基金项目（30871837）

The Expression of FcRn mRNA in Mammary Gland Epithelial Cells of Holstein Cows Treated with Different Concentrations of Thyroxine, Insulin, and Glucagon in Vitro

HU Xiao-Li, ZHANG Chun-Lin, WANG Jia-Qi, ZHAO Sheng-Guo, ZHANG Yu-Tao, BU Deng-Pan, ZHOU Ling-Yun

1.中国农业科学院北京畜牧兽医研究所/动物营养学国家重点实验室

Received:2010-08-30 Online:2011-10-15 Published:2010-11-26

摘要/Abstract

摘要： 【目的】采用real-time PCR方法检测体外激素处理对奶牛乳腺上皮细胞FcRn（neonatal Fc receptor，FcRn）mRNA表达的影响。【方法】在乳腺上皮细胞培养液中添加不同浓度胰高血糖素（0.01、0.1和1 μmol•L-1）、胰岛素（0.005、0.1和0.5 μmol•L-1）、甲状腺素T4（0.01、0.1和1 μmol•L-1），体外培养并收集细胞，提取细胞mRNA后，利用real-time PCR检测FcRn mRNA的相对丰度。【结果】在一定添加剂量范围内，随着添加剂量的增加，甲状腺素和胰高血糖素能够显著地促进FcRn mRNA表达量升高（P＜0.05），胰岛素在添加量为0.005 μmol•L-1和0.5 μmol•L-1时FcRn mRNA的表达量低，但在0.1 μmol•L-1添加时表达量显著升高（P＜0.05）。【结论】添加外源胰岛素、胰高血糖素和甲状腺素能够影响FcRn mRNA的表达，为进一步研究乳腺内FcRn受体变化以及影响因素提供了依据。

关键词: 荷斯坦奶牛, 乳腺上皮细胞, 激素, real-timePCR, mRNA

Abstract: 【Objective】The mRNA expression of FcRn was studied in Holstein Cows mammary gland epithelial cells treated with hormone by real-time PCR. 【Method】 FcRn mRNA expression of in vitro cultured mammary epithelial cells treated with different concentrations of thyroxine hormone (0.01, 0.1, 1 μmol•L-1), insulin (0.005, 0.1, 0.5 μmol•L-1) and glucagon (0.01, 0.1, 1 μmol•L-1), respectively, was determined.【Result】The results showed that the mRNA abundance of FcRn increased with the higher dose of thyroxine and glucagon significantly (P＜0.05) in a certain range. However, the mRNA expression of FcRn was significantly lower when treated with 0.005 μmol•L-1 and 0.5 μmol•L-1 insulin than 0.1μmol•L-1 (P＜0.05).【Conclusion】Thyroxine, insulin and glucagon can obviously change the level of FcRn mRNA, which provide a basis for further research of the change and influence of FcRn.

Key words: HolsteinCows, mammaryepithelialcells, hormone, real-timePCR, mRNA

胡小丽, 张春林, 王加启, 赵圣国, 章玉涛, 卜登攀, 周凌云. 不同剂量胰岛素、胰高血糖素和甲状腺素处理对奶牛乳腺上皮细胞FcRn mRNA表达的影响[J]. 中国农业科学, 2011, 44(20): 4279-4283.

HU Xiao-Li, ZHANG Chun-Lin, WANG Jia-Qi, ZHAO Sheng-Guo, ZHANG Yu-Tao, BU Deng-Pan, ZHOU Ling-Yun. The Expression of FcRn mRNA in Mammary Gland Epithelial Cells of Holstein Cows Treated with Different Concentrations of Thyroxine, Insulin, and Glucagon in Vitro[J]. Scientia Agricultura Sinica, 2011, 44(20): 4279-4283.

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不同剂量胰岛素、胰高血糖素和甲状腺素处理对奶牛乳腺上皮细胞FcRn mRNA表达的影响

The Expression of FcRn mRNA in Mammary Gland Epithelial Cells of Holstein Cows Treated with Different Concentrations of Thyroxine, Insulin, and Glucagon in Vitro

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