中国农业科学 ›› 2011, Vol. 44 ›› Issue (6): 1301-1306 .

• 研究简报 • 上一篇    

脐橙果皮油斑病解剖结构

  

  1. (西南大学园艺园林学院)
  • 收稿日期:2010-07-20 修回日期:2010-08-17 出版日期:2011-03-15 发布日期:2011-03-15
  • 通讯作者: 李道高

Anatomical Structures of Oleocellosis of Navel Orange Peel

  1. (西南大学园艺园林学院)
  • Received:2010-07-20 Revised:2010-08-17 Online:2011-03-15 Published:2011-03-15
  • Contact: LI Dao-gao

摘要:

【目的】通过对重庆奉节两个脐橙品种果皮油斑病两种处理下不同发病时期的调查和解剖结构的观察,比较涂蜡和套袋两种措施对油斑病的影响,探索油斑病发病的解剖学机理及预防措施。【方法】采用塑料切片技术,经过JB-4渗透、包埋,玻璃刀切片,PAS-TBO双重染色,显微镜观察、测定表皮细胞、组织结构特性。【结果】脐橙油胞分布在外果皮中,健康和发病初期油胞基本上保持其完整性而不发生破裂;发病晚期,破裂开口。油斑病的发生程度与果皮角质膜质地是否均匀一致有关;角质膜厚度的差异不是油斑病发生的直接原因。涂蜡的果皮质地均匀、外表光滑,保鲜效果好,但对油斑病的发生影响较小;套袋果感染其它病的机会较少,而且质地均匀、外表光滑,果皮的角质层较完整。【结论】强光照、外界压力、表皮外带水分、机械损伤都会增加油斑病的发生;套袋有助于降低油斑病发生。

关键词: 脐橙, 果皮, 油斑病, 解剖特征, 涂蜡, 套袋

Abstract:

【Objective】The aim of the paper is to explore the anatomical structures and preventive measures of oleocellosis through researching the anatomical structure of two navel orange varieties from Chongqing’s Fengjie in different treatments and compare the effects of waxing or bagging on oleocellosis.【Method】 Plastics microtomy was used in this research through JB-4 permeating, embedding, and slicing with glass knife, double-staining by PAS-TBO, and peel cells and characteristics of organizational structure were observed and determined by microscope.【Result】No rupture was observed in oil cavities exist in the epicarp in the healthy or the early onset, but rupture was observed in late period in illness samples. The occurrence degree of oleocellosis related to the degree of uniformity of peel corneous layer rather than the thickness. Uniform texture, smooth appearance, good effect of retaining freshness, but waxing did not affect the occurrence of oleocellosis. Bagging lessened the chance of other disease infection, and the texture was uniform, the appearance was smooth, and the peel cuticle was more complete.【Conclusion】Strong light, external pressure, peel moisture and mechanical damage all could increase the probability of oleocellosis. Bagging may help to reduce the occurrence probability of oleocellosis.

Key words: navel orange, peel, oleocellosis, anatomical character, waxing, bagging