中国农业科学 ›› 2005, Vol. 38 ›› Issue (08): 1578-1584 .

• 植物保护 • 上一篇    下一篇

玉米小斑病菌T小种培养滤液对玉米抗病性的诱导

马春红,陈霞,李广敏,翟彩霞,王立安,郭秀林,崔四平   

  1. 河北省农林科学院遗传生理研究所
  • 收稿日期:2004-06-25 修回日期:1900-01-01 出版日期:2005-08-10 发布日期:2005-08-10
  • 通讯作者: 李广敏

Induced Resistance by the Filtration of Bipolaris maydis Race T Toxin Cultivation

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  1. 河北省农林科学院遗传生理研究所
  • Received:2004-06-25 Revised:1900-01-01 Online:2005-08-10 Published:2005-08-10

摘要: 以1对同核异质的玉米C103为试材,进行了利用低浓度玉米小斑病菌T小种培养滤液作为激发子来提高玉米抗病性的研究。结果表明, 经预处理叶片的病斑面积为(0.3±0.05)~(0.9±0.5)mm2,而对照为(23.1±8.7)mm2,差异极显著。同时还测定了与提高抗病性有关酶的变化,经0~96 h的动态检测,以1∶50的处理效果为最好,苯丙氨酸解氨酶(PAL)活性比对照平均提高了64.2%,过氧化物酶(POD)活性比对照平均提高了41.2%,有害物质丙二醛(MDA)的含量比对照平均降低了29.7%。从而说明低浓度培养滤液本身能够成功地作为激发子来诱导玉米的系统获得性抗性。

关键词: 玉米小斑病菌T小种, 粗毒素(培养滤液), 苯丙氨酸解氨酶, 过氧化物酶, 丙二醛, 诱导抗性

Abstract: Resistance to maize southern leaf spot disease was induced by the low concentration of filtration of Bipolaris maydis race T cultivation in an experiment. The nuclear neterogeny corn C103 was used as the test material .The lesion area on the leaves was significant difference by connalysis The lesion areas on pretreated leaves were (0.3±0.05)-(0.9±0.5) mm2, but that in the control were (23.1±8.7)mm2. At the same time, the changes of peroxidase, phenylalanine, ammonialyase, malondialdenvde activities were determined. During 0-96 h inspection, phenylalanine, ammonialyase activities increased by 64.2%, peroxidase activities increased by 41.2%, but the malondialdenvde content decreased by 29.7% compared with the control. It seems that the low concentration of filftration of B. maydis race T cultivation itself can be used as an elicitor to enhance the induced resistance.

Key words: Bipolaris maydis race T, Filtration of cultivation, PAL, POD, MDA, Induced resistance