关键词: DES, 成年仓鼠, 睾丸, 生精细胞, 凋亡

Abstract: To explore the effects of diethylstilbestrol (DES) an environment endocrine disruptor, on the spermatogenesis of adult hamster, 10 adult hamsters were treated with DES (1mg·kg-1 body weight) daily by subcutaneous injection for one week. Morphological changes on adult hamster testis and spermatogenetic cells were observed by light and transmission electron microscope. Apoptotic spermatogenetic cells were detected by TUNEL staining and methyl green-pyronin staining. The results showed that the testis weight and seminiferous tubule area decreased significantly (P<0.01). Germ cells distributed disorderly in seminiferous tubule. Few long spermatids were found in almost all of the tubular lumen. Testis regression was associated with an important increase of apoptosis, especially in spermatocytes and spermatids. A lot of flocculent dense bodies or vacuole structures were observed in the cytoplasm of degenerating spermatocytes and spermatids by transmission electron microscope. In conclusion, this study indicates that DES has induced spermatogenetic cell apoptosis and it is an important cause of the abnormal spermatogenesis of adult hamster testis.

Key words: Diethylstilbestrol, Adult hamster, Testes, Spermatogenetic cells, Apoptosis