粒用高粱幼苗期耐苏打盐碱综合评价与指标鉴选

doi:10.3864/j.issn.0578-1752.2025.01.003

中国农业科学 ›› 2025, Vol. 58 ›› Issue (1): 30-42.doi: 10.3864/j.issn.0578-1752.2025.01.003

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

粒用高粱幼苗期耐苏打盐碱综合评价与指标鉴选

乔钲岩¹^,²(), 于淼¹(), 唐玉劼¹, 石贵山¹, 刘新宇¹^,², 刘晓涵¹^,³, 王新鼎¹^,², 李扬¹, 王鼐¹^,^*(), 陈冰嬬¹^,^*()

¹ 吉林省农业科学院作物资源研究所，吉林公主岭 136100
² 吉林农业大学农学院，长春 130118
³ 齐齐哈尔大学生命科学与农林学院，黑龙江齐齐哈尔 161000

收稿日期:2024-06-09 接受日期:2024-09-02 出版日期:2025-01-01 发布日期:2025-01-07
通信作者:
王鼐，E-mail：wang-nai@163.com。
陈冰嬬，E-mail：chenbingru1979@163.com。
联系方式: 乔钲岩，E-mail：15039961556@163.com。于淼，E-mail：yumiao910816@163.com。乔钲岩和于淼为同等贡献作者。
基金资助:
吉林省科技发展计划(20240601065RC); 农业农村部国家现代农业产业技术体系建设专项(CARS-06)

Comprehensive Evaluation for Soda Salinity and Alkalinity in Sorghum Seedling Stage and Selection of Indicators

QIAO ZhengYan¹^,²(), YU Miao¹(), TANG YuJie¹, SHI GuiShan¹, LIU XinYu¹^,², LIU XiaoHan¹^,³, WANG XinDing¹^,², LI Yang¹, WANG Nai¹^,^*(), CHEN BingRu¹^,^*()

¹ Crop Resources Research Institute of Jilin Academy of Agricultural Sciences, Gongzhuling 136100, Jilin
² College of Agronomy, Jilin Agricultural University, Changchun 130118
³ College of Life Sciences and Agriculture and Forestry, Qiqihar University, Qiqihar 161000, Heilongjiang

Received:2024-06-09 Accepted:2024-09-02 Published:2025-01-01 Online:2025-01-07

摘要/Abstract

摘要：

【目的】筛选苏打盐碱胁迫液浓度和耐盐碱测定指标，建立大批量耐盐碱鉴定方法；对粒用高粱核心种质的耐盐碱特性进行综合评价，筛选耐盐碱种质，为进一步培育耐盐碱亲本和杂交种提供种质基础。【方法】松嫩平原盐碱土的主要成分是Na₂CO₃和NaHCO₃。采用50 mmol·L^-1 NaHCO₃﹕Na₂CO₃=9﹕1为胁迫液模拟松嫩平原中度盐碱土环境，胁迫液的pH为9.19、含盐量为0.21%。以苗高、根长、苗鲜重、根鲜重、苗干重、根干重、根冠比鲜重、根冠比干重8个性状为测定指标，鉴定285份粒用高粱核心种质苗期的耐盐碱特性。采用主成分分析筛选粒用高粱苗期耐盐碱鉴定指标并建立幼苗期耐盐碱评价数学模型，采用聚类分析将285份高粱种质的耐盐碱特性分级，筛选出耐盐碱性较强的种质。【结果】50 mmol·L^-1盐碱胁迫对285份高粱种质的8个指标均表现出抑制作用，8个性状的平均耐盐碱系数分别为0.794、0.785、0.565、0.554、0.802、0.638、0.978和0.841，并且盐碱胁迫下各指标之间均呈现出显著正相关性；主成分分析筛选出苗高、根鲜重可作为高粱幼苗期苏打盐碱胁迫鉴定评价的指标；采用多元线性回归分析归纳出高粱幼苗期耐盐碱特性评价模型Y=0.097X4+0.171X2+ 0.201X6+0.157X1+0.105X3-0.147，可用于多个指标的综合评价；聚类分析将285份粒用高粱核心种质耐盐碱特性划分为强耐盐碱、耐盐碱、中间型、敏感型、极敏感型5个等级，其中，强耐盐种质8份、耐盐碱种质8份、中间型种质112份、敏感型种质134份、极敏感型种质23份。将耐盐碱种质和极敏感型种质种植在吉林省西部苏打盐碱地中度盐碱土（pH 8.5—9.5、含盐量0.3%）进行验证，强耐盐碱种质平均出苗率为45.4%、平均苗高为23 cm；耐盐碱种质平均出苗率为31.3%、平均苗高20.9 cm；极敏感型种质平均出苗率为20%、平均苗高12.3 cm。【结论】以50 mmol·L^-1苏打盐碱浓度（NaHCO₃﹕Na₂CO₃=9﹕1）从285份高粱种质资源中筛选出8份强耐苏打盐碱的种质，8份耐盐碱种质，筛选出苗高和根鲜重2个性状作为高粱幼苗期大批量耐盐碱筛选的鉴定评价指标。

关键词: 苏打盐碱土, 高粱, 苗期, 耐盐碱, 综合评价

Abstract:

【Objective】This study was conducted to screen the concentration of soda saline-alkali stress solution and the determination index of saline-alkali tolerance, to establish the identification method of saline-alkali tolerance to large-scale identification of sorghum germplasms. The saline-alkali tolerance of the core germplasms of grain sorghum were comprehensively evaluated, and the saline-alkali tolerant germplasms were identified and selected to provide a germplasm basis for further breeding of saline-alkali tolerant parents and hybrids.【Method】The main components of saline-alkali soil of in The Songnen Plain are Na₂CO₃ and NaHCO₃. This experiment used 50 mmol·L^-1 NaHCO₃﹕Na₂CO₃=9﹕1 as a stress solution to simulate the moderate saline-alkali environment in Songnen Plain, the pH is 9.19, Salinity is 0.21%. Eight traits, including seedling height, root length, seedling fresh weight, root fresh weight, seeding dry weight, root dry weight, root-shoot ratio fresh weight and root-shoot ratio dry weight were used as measurement indexes and the saline-alkali tolerance characteristics of 285 sorghum core germplasms at seedling stage were identified. Principal component analysis was used to screen the salt-alkali tolerance identification indexes of grain sorghum at seedling stage and establish a mathematical model for salt-alkali tolerance evaluation at seedling stage. The saline-alkaline tolerance of 285 sorghum germplasms was classified by cluster analysis, and the germplasms with strong saline-alkaline tolerance were screened.【Result】50 mmol·L^-1saline-alkali stress showed inhibitory effects on 8 indexes of 285 sorghum germplasms, the average values of saline -alkali resistance coefficients are 0.794, 0.785, 0.565, 0.554, 0.802, 0.638, 0.978, and 0.841.Under saline alkali stress, 8 indexes showed significant positive correlations; the seedling height and root fresh weight could be used as the indexes for the identification and evaluation of soda saline-alkali stress in sorghum seedling stage by principal component analysis; The evaluation model of saline-alkali tolerance characteristics of sorghum seedlings was summarized by multiple linear regression analysis Y=0.097X4+0.171X2+0.201X6+0.157X1+0.105X3- 0.147, it can be used for comprehensive evaluation of multiple indicators. 285 grain sorghum core collections were divided into 5 grades by cluster analysis, strong saline-alkali tolerance, saline-alkali tolerance, intermediate type, sensitive type and extremely sensitive type, Among them, there were 8 strong salt-tolerant germplasms, 8 salt-tolerant germplasms, 112 intermediate germplasms, 134 sensitive germplasms and 23 extremely sensitive germplasms. The saline-alkali tolerant germplasm and extremely sensitive germplasm were planted in the moderate saline-alkali soil (pH 8.5-9.5, Salinity 0.3%) for verification in Zhenlai County of western Jilin Province. The average seedling emergence rate of strong saline-alkali tolerant germplasm was 45.4%, and the average seedling height was 23 cm. The average seedling emergence rate of salt-tolerant germplasm was 31.3%, and the average height was 20.9 cm. The average emergence rate of extremely sensitive germplasm was 20%, and the average seedling height was 12.3 cm.【Conclusion】8 strong soda saline-alkali tolerant germplasms and 8 saline-alkali tolerant germplasms were screened out from 285 sorghum germplasms resources with 50 mmol·L^-1 soda saline-alkali concentration (NaHCO₃﹕Na2CO₃=9﹕1). The seedling height and root fresh weight could be used as the preferred evaluation indexes for the identification of saline-alkali tolerance at seedling stage.

Key words: soda saline alkali soil, sorghum, seedling stage, salt alkali resistance, comprehensive evaluation

乔钲岩, 于淼, 唐玉劼, 石贵山, 刘新宇, 刘晓涵, 王新鼎, 李扬, 王鼐, 陈冰嬬. 粒用高粱幼苗期耐苏打盐碱综合评价与指标鉴选[J]. 中国农业科学, 2025, 58(1): 30-42.

QIAO ZhengYan, YU Miao, TANG YuJie, SHI GuiShan, LIU XinYu, LIU XiaoHan, WANG XinDing, LI Yang, WANG Nai, CHEN BingRu. Comprehensive Evaluation for Soda Salinity and Alkalinity in Sorghum Seedling Stage and Selection of Indicators[J]. Scientia Agricultura Sinica, 2025, 58(1): 30-42.

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Na⁺浓度 Na⁺ concentration (mmol·L^-1)	pH	电导率 TDS (ms·cm^-1)	盐度Salinity (%)
0(CK)	7.18	0.33	0.00
25	9.15	2.21	0.11
50	9.19	4.04	0.21
75	9.33	5.80	0.31
100	9.21	7.49	0.40
150	9.29	10.63	0.58
200	9.27	17.75	1.01

序号 No.	性状 Traits	测定方法 Measurement method
1	苗高SH (cm)	用直尺测量最长叶片顶部到茎基部的距离 Measure the distance from the top of the longest leaf to the base of the stem with a ruler
2	根长RL (cm)	用直尺测量籽粒节点到主根根尖的距离 Measure the distance from the grain node to the root tip of the main root using a ruler
3	苗鲜重SFW (g)	用分析天平称量籽粒节点以上整个株苗的重量 Weigh the entire seedling above the grain node using an analytical balance
4	根鲜重RFW (g)	用分析天平称量籽粒节点以下整个根部的重量 Weigh the entire root below the grain node using an analytical balance
5	苗干重SDW (g)	测完鲜重的株苗于烘箱烘干，用分析天平称量干重 After measuring the fresh weight of the seedlings, dry them in an oven and weigh the dry weight using an analytical balance
6	根干重RDW (g)	测完鲜重的根部于烘箱烘干，用分析天平称量干重 After measuring the fresh weight of the roots, dry them in an oven and weigh the dry weight using an analytical balance
7	根冠比鲜重RSFW	根鲜重与苗鲜重的比值 Ratio of root fresh weight to seedling fresh weight
8	根冠比干重RSDW	根干重与苗干重的比值 Ratio of root dry weight to seedling dry weight

性状 Traits	最小值 Min	中位数 Median	最大值 Max	平均数 Mean	标准差 SD	偏度 Skew	峰度 Kurt	变异系数 CV (%)
苗高SH (cm)	0.340	0.798	1.411	0.794	0.144	0.350	1.202	18.17
根长RL (cm)	0.382	0.763	1.454	0.785	0.194	0.572	0.452	24.73
苗鲜重SFW (g)	0.096	0.536	1.581	0.565	0.211	0.819	1.742	37.36
根鲜重RFW (g)	0.102	0.521	1.573	0.554	0.264	0.882	1.035	47.75
苗干重SDW (g)	0.102	0.790	1.768	0.802	0.217	0.512	2.373	26.99
根干重RDW (g)	0.093	0.632	1.398	0.638	0.245	0.488	-0.053	38.43
根冠比鲜重RSFW	0.273	0.927	3.716	0.978	0.576	1.899	4.494	54.52
根冠比干重RSDW	0.117	0.753	3.740	0.841	0.404	2.607	12.482	48.04

指标 <BOLD>T</BOLD>raits	主成分Principal component
指标 <BOLD>T</BOLD>raits	PC1	PC2
苗高SH	0.813	-0.265
根长RL	0.654	0.094
苗鲜重SFW	0.686	-0.595
根鲜重RFW	0.935	0.101
苗干重SDW	0.662	-0.609
根干重RDW	0.869	0.266
根冠比鲜重RSFW	0.441	0.728
根冠比干重RSDW	0.420	0.759
特征值Eigen value	3.998	1.992
贡献率Contribution (%)	49.976	24.901
累计贡献率 Cumulative contribution (%)	49.976	74.877

多元回归方程 Multiple regression equation	相关系数 r	判定系数 R²	F值 F value	P值 P value
Y=0.433X4+0.106	0.908	0.825	776.288	≤0.001
Y=0.357X4+0.203X2-0.005	0.948	0.900	734.972	≤0.001
Y=0.211X4+0.194X2+0.184X6-0.037	0.971	0.943	899.738	≤0.001
Y=0.127X4+0.157X2+0.193X6+0.232X1-0.146	0.991	0.983	2345.029	≤0.001
Y=0.097X4+0.171X2+0.201X6+0.157X1+0.105X3-0.147	0.998	0.996	7601.871	≤0.001

粒用高粱幼苗期耐苏打盐碱综合评价与指标鉴选

Comprehensive Evaluation for Soda Salinity and Alkalinity in Sorghum Seedling Stage and Selection of Indicators

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