基于靶向代谢组学的山楂黄色和红色果皮呈色物质差异分析

doi:10.3864/j.issn.0578-1752.2024.12.013

中国农业科学 ›› 2024, Vol. 57 ›› Issue (12): 2439-2453.doi: 10.3864/j.issn.0578-1752.2024.12.013

基于靶向代谢组学的山楂黄色和红色果皮呈色物质差异分析

郭荣琨¹^,²(), 董宁光²(), 农惠兰¹^,², 王菡², 滕维超¹(), 孟佳欣²()

¹ 广西大学林学院/广西森林生态与保育重点实验室/广西高校亚热带人工林培育与利用重点实验室，南宁 530004
² 北京市农林科学院林业果树研究所/北京市落叶果树工程技术研究中心/农业农村部华北地区园艺作物生物学与种质创制重点实验室，北京 100093

收稿日期:2023-11-21 接受日期:2024-04-19 出版日期:2024-06-16 发布日期:2024-06-25
通信作者:
滕维超，E-mail：vincentt@yeah.net。
孟佳欣，E-mail：mengjiaxin26@163.com
联系方式: 郭荣琨，E-mail：18277136649@163.com。董宁光，E-mail：dongng@sina.com。郭荣琨和董宁光为同等贡献作者。
基金资助:
北京市农林科学院青年科研基金(QNJJ202409); 北京市农林科学院科技创新能力专项(KJCX20230101); 北京市农林科学院科技创新能力专项(KJCX20240102); 北京市农林科学院林业果树研究所青年科研基金(LGSJJ202302)

Targeted Metabolomics-Based Analysis of Peel Color Differences Between Yellow and Red Hawthorn

GUO RongKun¹^,²(), DONG NingGuang²(), NONG HuiLan¹^,², WANG Han², TENG WeiChao¹(), MENG JiaXin²()

¹ Guangxi Key Laboratory of Forest Ecology and Conservation/Guangxi Colleges and Universities Key Laboratory for Cultivation and Utilization of Subtropical Forest Plantation/College of Forestry, Guangxi University, Nanning 530004
² Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences/Beijing Engineering Research Center for Deciduous Fruit Trees/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture and Rural Affairs, Beijing 100093

Received:2023-11-21 Accepted:2024-04-19 Published:2024-06-16 Online:2024-06-25

摘要/Abstract

摘要：

【目的】探究黄色山楂和红色山楂成熟过程中果皮类黄酮和类胡萝卜素的积累模式，增加对山楂果皮呈色机理的认识，为果皮色泽定向遗传育种提供参考。【方法】以黄山楂‘金如意’（Crataegus pinnatifida Jinruyi）和红山楂‘软籽’（Crataegus pinnatifida Ruanzi）的果皮为试材，利用靶向代谢组学对2个品种5个生长时期果皮中的类黄酮和类胡萝卜素组分及含量进行定性定量研究，通过正交偏最小二乘分析（OPLS-DA）对不同果色品种果皮中积累的类黄酮和类胡萝卜素代谢物进行差异分析。【结果】两个山楂品种果皮中共检测出130种类黄酮代谢物和49种类胡萝卜素代谢物，这些物质在不同品种、不同生长时期内的山楂果皮中存在显著的种类及含量差异。‘软籽’山楂果皮中花青素含量远高于‘金如意’山楂果皮，成熟期（S5）花青素化合物在红色山楂和黄色山楂果皮中的总含量分别为930.04和2.32 µg·g^-1，两个品种山楂果皮中花青素含量相差约400倍。矢车菊素-3-O-半乳糖苷是最主要的花青素成分，其在花青素代谢物总含量中占比高达95%以上。类胡萝卜素代谢分析显示，‘软籽’和‘金如意’S1—S3时期果皮总类胡萝卜素含量无显著差异，主要积累的类胡萝卜素成分均为叶黄素。S4—S5时期，两种山楂果皮中类胡萝卜素含量差异逐渐扩大，β-胡萝卜素、β-隐黄质、新黄质、α-胡萝卜素和玉米黄质在‘金如意’果皮中大量积累，其含量高于‘软籽’山楂。【结论】花青素积累是导致‘软籽’山楂果皮在成熟期呈现红色的主要原因，类胡萝卜素的积累是‘金如意’山楂发育后期黄色加深的主要原因，花青素和类胡萝卜素的差异性积累是造成山楂果皮颜色差异的关键因素。

关键词: 山楂, 花青素, 类黄酮, 类胡萝卜素, 代谢组学, 果色

Abstract:

【Objective】 The aim of this study was to explore the accumulation pattern of flavonoids and carotenoids of yellow and red hawthorn peel during the fruit ripening process, as well as to deepen the understanding of the coloration mechanism of hawthorn peel, so as to provide the guidance for peel color-directed genetic breeding. 【Method】The fruit peel of yellow hawthorn (Crataegus pinnatifida Jinruyi) and red hawthorn (Crataegus pinnatifida Ruanzi) was used as test materials. The components and content of flavonoid and carotenoid in the peel of the two cultivars during different growth stages were examined qualitatively and quantitatively based on targeted metabolomics analysis. The differential accumulation of flavonoids and carotenoid metabolites between the two cultivars was analyzed by the orthogonal partial least squares discriminant analysis (OPLS-DA). 【Result】A total of 130 kinds of flavonoids and 49 kinds of carotenoids were detected in the peel of the two hawthorn cultivars, and the components and content of these metabolites were significantly different between the two cultivars during different growth stages. The anthocyanin content of the Ruanzi peel was 400 times higher than that of the Jinruyi peel, which in the Ruanzi peel and Jinruyi peel at the mature period (S5) was 930.04 and 2.32 µg·g^-1, respectively. The major anthocyanin species was cyanidin-3-O-galactoside, which accounted for more than 95% of total anthocyanins. The carotenoid metabolism analysis showed that there was no significant difference in total carotenoid content between Ruanzi and Jinruyi during the S1-S3 period, and lutein was the predominant accumulated carotenoid. The content of β-carotene, β-cryptoxanthin, neoxanthin, α-carotene, and zeaxanthin in the Jinruyi peel were significantly higher than that in the Ruanzi peel during the S4-S5 period. The massive accumulation of these carotenoid metabolites in the Jinruyi peel led to the difference in carotenoid content in the two hawthorn cultivars during the S4-S5 period. 【Conclusion】 In the hawthorn fruit mature period, the accumulation of anthocyanin in the Ruanzi peel led to the appearance of red, and the accumulation of carotenoid in the Jinruyi peel led to the appearance of yellow. The differential accumulation of anthocyanins and carotenoids played an important role in the hawthorn peel different coloration.

Key words: hawthorn, anthocyanin, flavonoid, carotenoid, metabolomics, peel color

郭荣琨, 董宁光, 农惠兰, 王菡, 滕维超, 孟佳欣. 基于靶向代谢组学的山楂黄色和红色果皮呈色物质差异分析[J]. 中国农业科学, 2024, 57(12): 2439-2453.

GUO RongKun, DONG NingGuang, NONG HuiLan, WANG Han, TENG WeiChao, MENG JiaXin. Targeted Metabolomics-Based Analysis of Peel Color Differences Between Yellow and Red Hawthorn[J]. Scientia Agricultura Sinica, 2024, 57(12): 2439-2453.

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基于靶向代谢组学的山楂黄色和红色果皮呈色物质差异分析

Targeted Metabolomics-Based Analysis of Peel Color Differences Between Yellow and Red Hawthorn

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