中国农业科学 ›› 2020, Vol. 53 ›› Issue (18): 3693-3706.doi: 10.3864/j.issn.0578-1752.2020.18.006
黄苗苗1,2,3(),陈万权1,2,4(
),曹世勤4,5,孙振宇4,5,贾秋珍4,5,高利2,4,刘博2,4,刘太国2,3,4(
)
收稿日期:
2020-01-20
接受日期:
2020-03-20
出版日期:
2020-09-16
发布日期:
2020-09-25
通讯作者:
陈万权,刘太国
作者简介:
黄苗苗,E-mail: 基金资助:
HUANG MiaoMiao1,2,3(),CHEN WanQuan1,2,4(
),CAO ShiQin4,5,SUN ZhenYu4,5,JIA QiuZhen4,5,GAO Li2,4,LIU Bo2,4,LIU TaiGuo2,3,4(
)
Received:
2020-01-20
Accepted:
2020-03-20
Online:
2020-09-16
Published:
2020-09-25
Contact:
WanQuan CHEN,TaiGuo LIU
摘要:
【目的】了解甘肃和青海小麦条锈菌(Puccinia striiformis f. sp. tritici)春季流行传播路线、群体遗传多样性和生殖模式,明确春季流行期两省小麦条锈菌的传播关系及菌源交流规律,进而为两省小麦条锈病的预测预报、确定越夏初始菌源来源和有效治理提供理论依据。【方法】选择条锈病常发生的地区作为调查和研究区域。甘肃省4个试验点:陇南市文县、陇东平凉市崆峒区、中部麦区定西市临洮县、临夏州临夏县;青海省2个试验点:西宁市城北区、海东市互助县。2017年秋季,在甘肃和青海省6个试验点内根据当地小麦播种适期依次种植82份变异观察圃材料。2018年4—8月,对试验点82份变异观察圃材料进行田间病害调查,并采集到551份小麦条锈菌标样,使用15对引物进行SSR分子标记分析。利用GenAlEx和POPPR v2.5.0软件对数据进行相关分析, 不显著的rbarD值表示连锁平衡,用于推断群体是否发生有性重组。【结果】82份变异观察圃材料在甘肃地区发病比青海地区严重。15对引物组合共扩增出81个位点,每对引物组合产生的多态性位点为2—12个。551份样本克隆矫正后,共鉴定出505个多位点基因型(MLG),其中仅有32个MLG被克隆并进行了2—6次重新采样。甘肃和青海群体总的基因型多样性(G=0.917)较高,其中,甘肃平凉群体的最高,青海互助群体次之,甘肃临洮群体最低。小麦条锈菌的遗传变异主要在各群体内部个体之间。春季流行期,菌源在各群体之间交流频繁,青海东部(互助和西宁)群体与甘肃(平凉和临夏)群体之间的基因流高于青海(互助和西宁)群体与甘肃文县群体之间的基因流。最小时空网络图(MSN)和非参数主成分分析(DAPC)表明青海互助和西宁的群体与来自于甘肃平凉和临夏的群体之间菌源关系最密切,差异最小;与临洮群体遗传距离相对较远且临洮群体相对独立;文县群体则是一个完全独立的群体,与其他5个群体之间的差异最大。连锁不平衡分析表明,甘肃文县、临夏和青海西宁群体存在不显著的rbarD值表示连锁平衡,是有性生殖群体,其中文县群体(rbarD=0.0139,P=0.186)显示出明显的有性重组特征。【结论】小麦条锈病春季流行期,甘肃地区与青海东部地区的传播路线以甘肃平凉、临夏到青海的传播为主,甘肃文县到青海的传播为辅。甘肃文县、临夏和青海西宁3个群体存在有性生殖现象,对甘肃、青海地区条锈菌丰富的遗传多样性的形成具有一定作用。
黄苗苗,陈万权,曹世勤,孙振宇,贾秋珍,高利,刘博,刘太国. 甘肃、青海地区小麦条锈菌监测及群体遗传多样性分析[J]. 中国农业科学, 2020, 53(18): 3693-3706.
HUANG MiaoMiao,CHEN WanQuan,CAO ShiQin,SUN ZhenYu,JIA QiuZhen,GAO Li,LIU Bo,LIU TaiGuo. Surveillance and Genetic Diversity Analysis of Puccinia striiformis f. sp. tritici in Gansu and Qinghai Provinces[J]. Scientia Agricultura Sinica, 2020, 53(18): 3693-3706.
表1
甘肃、青海两省6个观察圃内小麦条锈病自然发病调查结果及采集的551份标样信息"
试验点 Test site | 文县 Wenxian | 平凉(崆峒区) Pingliang (Kongtong) | 临夏 Linxia | 临洮 Lintao | 互助 Huzhu | 西宁(城北区) Xining (Chengbei) | |
---|---|---|---|---|---|---|---|
播种时间(年-月-日) Day of seeding time (Year-month-day) | 2017-10-08 | 2017-09-25 | 2017-09-28 | 2017-10-08 | 2018-04-04 | 2018-04-25 | |
采集时间(年-月-日) Day of collection (Year-month-day) | 2018-04-14 | 2018-05-24 | 2018-06-16 | 2018-06-18 | 2018-08-08 | 2018-08-09 | |
经度 Longitude | 104°53′17″ | 106°40′11″ | 103°11′13″ | 103°31′18″ | 101°53′24″ | 101°44′56″ | |
纬度 Latitude | 32°50′43″ | 35°33′06″ | 35°36′49″ | 35°13′24″ | 36°42′41″ | 36°43′15″ | |
海拔 Elevation (m) | 786 | 1190 | 2010 | 1893 | 2360 | 2350 | |
样本数量 Number of samples | 27 | 132 | 156 | 178 | 47 | 11 | |
编号 Code | 品种名称 Cultivar | IT/S/I (N) | |||||
1 | 水源11 Suwon 11 | 3/5/T (1) | 3/5/T (7) | 4/60/80 (5) | 4/10/10 (3) | 0 | 0 |
2 | 洛夫林10 Lovrin 10 | 3/5/T (1) | 3/5/10 (8) | 4/40/60 (6) | 4/10/T (4) | 0 | 0 |
3 | 洛夫林13 Lovrin 13 | 0 | 3/20/40 (4) | 4/20/60 (6) | 4/20/T (4) | 0 | 0 |
4 | 抗引655 Kangyin 655 | 0 | 3/10/20 (5) | 4/20/40 (5) | 0 | 0 | 0 |
5 | 中四(无芒)Zhong 4 (un-awn) | 0 | 0 | 4/40/60 (5) | 0 | 0 | 0 |
6 | 贵农22 Guinong 22 | 0 | 4/10/10 (2) | 0 | 4/T/T (2) | 0 | 0 |
7 | Triticum spelta album | 0 | 0 | 0 | 0 | 0 | 0 |
8 | Hybrid 46 | 0 | 0 | 0 | 4/10/10 (3) | 0 | 0 |
9 | Reichersberg 42 | 0 | 0 | 4/10/10 (3) | 4/10/T (3) | 0 | 0 |
10 | Heines Peko | 0 | 3/5/10 (6) | 4/60/80 (5) | 4/10/20 (4) | 0 | 0 |
11 | Nord Desprez | 0 | 0 | 4/80/80 (6) | 4/10/T (3) | 4/10/T (2) | 0 |
12 | Compair | 0 | 3/5/10 (2) | 4/40/40 (5) | 4/10/10 (3) | 2/T/T (1) | 3/T/T (1) |
13 | Carsten V | 0 | 3/5/T (3) | 4/20/20 (6) | 0 | 3/T/T (2) | 0 |
14 | Spaldings Prolific | 0 | 3/10/T (4) | 4/10/20 (4) | 4/10/T (2) | 0 | 0 |
15 | Heines Ⅶ | 0 | 0 | 4/10/20 (4) | 0 | 0 | 0 |
16 | Joss Cambier | 0 | 0 | 0 | 0 | 0 | 0 |
17 | Mega | 0 | 0 | 0 | 3/5/T (2) | 0 | 0 |
18 | Hobbit | 0 | 3/T/T (4) | 4/20/40 (5) | 3/10/T (4) | 3/40/20 (4) | 0 |
19 | 铭贤169 Mingxian 169 | 4/60/40 (1) | 4/40/20 (7) | 4/60/60 (4) | 4/20/10 (3) | 4/40/20 (2) | 4/40/40 (3) |
20 | 铭贤169*6/Yr5 Mingxian 169*6/Yr5 | 0 | 3/10/T (2) | 4/60/60 (6) | 0 | 0 | 0 |
21 | 铭贤169*6/Yr10 Mingxian 169*6/Yr10 | 0 | 4/10/10 (5) | 4/80/80 (4) | 4/10/10 (6) | 4/60/60 (7) | 0 |
22 | 周麦22 Zhoumai 22 | 0 | 0 | 0 | 0 | 4/10/10 (4) | 0 |
23 | 烟农15 Yannong 15 | 0 | 3/5/T (5) | 4/80/80 (2) | 4/20/20 (4) | 0 | 0 |
24 | 鲁麦23 Lumai 23 | 3/10/10 (4) | 4/20/10 (2) | 4/80/80 (4) | 4/60/40 (6) | 0 | 0 |
25 | 冀麦38 Jimai 38 | 0 | 4/10/20 (6) | 4/20/80 (6) | 4/20/10 (6) | 0 | 0 |
26 | 石4185 Shi 4185 | 0 | 3/10/10 (3) | 4/20/20 (5) | 4/60/30 (3) | 4/T/T (3) | 0 |
27 | 晋太170 Jintai 170 | 0 | 4/20/20 (1) | 4/60/40 (3) | 4/20/40 (4) | 4/10/20 (3) | 0 |
28 | 杨麦158 Yangmai 158 | 0 | 3/5/T (2) | 4/20/20 (5) | 0 | 0 | 0 |
29 | 兰天19 Lantain 19 | 3/5/T (4) | 0 | 0 | 0 | 0 | 0 |
30 | 兰天31 Lantian 31 | 3/5/T (1) | 0 | 4/T/T (2) | 0 | 0 | 0 |
31 | 兰天26 Lantian 26 | 3/10/T (4) | 0 | 0 | 0 | 0 | 0 |
32 | 天选50 Tianxuan 50 | 3/5/T (1) | 0 | 0 | 0 | 0 | 0 |
33 | 陇鉴386 Longjian 386 | 0 | 0 | 4/T/T (2) | 0 | 4/40/60 (7) | 0 |
34 | Chancellor | 0 | 4/20/20 (1) | 0 | 4/20/20 (4) | 4/30/40 (7) | 0 |
35 | Ulka/8cc | 0 | 4/20/30 (5) | 0 | 3/5/T (1) | — | 0 |
36 | Maris Huntsman | 0 | — | 4/10/10 (2) | — | 0 | 0 |
37 | 小白冬麦 Xiaobaidongmai | 0 | 3/10/T (1) | 0 | 4/40/10 (2) | 0 | 0 |
38 | Khaplic/8cc | 0 | 3/10/10 (1) | 0 | 4/10/10 (5) | 0 | 0 |
39 | Armada | 0 | 0 | 0 | 0 | 0 | 0 |
40 | 81-7241 | 0 | 3/20/20 (4) | 4/10/T (4) | 4/80/40 (3) | — | 0 |
41 | Aquila | 0 | — | 4/5/10 (2) | — | 4/20/20 (3) | — |
42 | 赤牙糙 Chiyacao | 0 | 4/10/30 (4) | 4/10/10 (2) | 4/80/60 (4) | 0 | 0 |
43 | 蚂蚱麦 Mazhamai | 0 | 3/10/T (3) | 4/10/T (3) | 4/5/10 (3) | 0 | — |
44 | 92R137 | 0 | 0 | 4/10/10 (5) | 4/T/T (2) | 0 | 0 |
45 | 保丰104 Baofen 104 | 0 | 3/10/T (3) | 4/10/T (1) | 4/40/40 (5) | 0 | 4/40/20 (5) |
46 | 高加索 Gaojiasuo | 0 | 0 | 4/20/10 (4) | 0 | 0 | 0 |
47 | 京双16 Jingshuang 16 | 0 | 3/20/10 (1) | 4/10/10 (1) | 0 | 0 | 0 |
48 | 白兔3号 Baitu No. 3 | 0 | 3/10/20 (5) | 4/10/10 (3) | 4/10/10 (2) | 0 | — |
49 | 绵麦37 Mianmai 37 | 0 | 0 | 4/10/10 (3) | 0 | 0 | 0 |
50 | 繁6 Fan 6 | 0 | 4/5/T (3) | 4/T/T (3) | 4/10/T (3) | 0 | 0 |
51 | 绵阳11 Mianyang 11 | 0 | 3/40/40 (5) | 4/T/T (3) | 4/10/T (3) | 0 | 0 |
52 | 川麦107 Chuanmai 107 | 3/10/T(2) | 2/5/T (2) | 4/10/T (1) | 4/T/T (1) | 0 | 0 |
53 | 川农19 Chuannong 19 | 3/5/T(4) | 0 | 4/20/T (1) | 0 | 0 | 0 |
54 | 川农27 Chuannong 27 | 3/5/T(2) | 0 | 4/20/10 (4) | 0 | 0 | 0 |
55 | 川麦58 Chuanmai 58 | 3/5/T(1) | 0 | 0 | 4/T/T (2) | 0 | 0 |
56 | 川育23 Chuanyu 23 | 0 | 0 | 0 | 0 | 0 | 0 |
57 | Avocet S*6/Yr9 | 0 | 0 | 4/5/10 (2) | 0 | 0 | 0 |
58 | Avocet S*6/Yr15 | 0 | 0 | 4/10/10 (2) | — | 0 | 0 |
59 | Avocet S*6/Yr24 | 0 | 0 | 0 | 0 | 0 | 0 |
60 | Avocet S*6/Yr26 | 0 | 0 | 0 | 0 | 0 | 0 |
61 | Jupateco R Yr18 | 0 | 0 | 0 | 0 | 0 | 0 |
62 | C591 | 0 | 0 | 4/10/10 (3) | 0 | 0 | 0 |
63 | TcLr9 | 0 | 0 | 0 | 4/10/T (4) | 0 | 0 |
64 | TcLr16 | 0 | 0 | 4/T/T (2) | 4/40/10 (3) | 0 | 0 |
65 | TcLr19 | 0 | 4/40/10 (1) | 4/10/10 (3) | 0 | 0 | 0 |
66 | TcLr24 | 0 | 3/10/10 (4) | 4/10/10 (2) | 0 | 0 | 0 |
67 | TcLr26 | 0 | 3/5/T (4) | 4/10/T (2) | 0 | 0 | 0 |
68 | TcLr38 | 0 | 0 | 0 | 0 | 0 | 0 |
69 | TcLr46 | 0 | 0 | 4/60/40 (3) | 0 | 0 | 0 |
70 | ISr5 Ra | 0 | 0 | 4/60/80 (4) | 0 | 0 | 0 |
71 | ISr6 Ra | 0 | 0 | 0 | 0 | 0 | 0 |
72 | Verstein Sr9e | 0 | 3/40/20 (6) | 0 | — | 0 | 0 |
73 | W2691Sr10 | 0 | 4/10/10 (6) | 4/10/20 (2) | 4/20/40 (5) | 0 | 0 |
74 | ISr11 Ra | 0 | 0 | 0 | 0 | 0 | 0 |
75 | CnS-T-mono-deri=Sr21 | 0 | 3/T/T (3) | 0 | 0 | 0 | 0 |
76 | LcSr24Ag | 0 | 3/20/T (5) | 4/10/T (1) | 4/10/10 (3) | 0 | 0 |
77 | Ealge Sr26 | 0 | 2/5/T (1) | 4/10/10 (1) | 4/5/T (1) | 0 | 0 |
78 | BtSr30 Wst | 0 | 3/10/T (2) | 0 | 0 | 0 | 0 |
79 | Sr31/6*LMPG | 3/5/T (1) | 2/T/T (2) | 4/5/T (1) | 4/5/T (3) | 0 | 0 |
80 | W2691 SrTt-1=Sr36 | 0 | 3/10/T (5) | 4/5/T (1) | 4/5/T (2) | 0 | 0 |
81 | Trident Sr38 | 0 | 0 | 0 | 0 | 0 | 0 |
82 | Little Club | 0 | 4/20/30 (1) | 0 | 0 | 0 | 4/T/T (2) |
表2
本试验使用的15对SSR小麦条锈菌引物信息"
引物名称 Primer name | 重复单元 Repeat motif | 引物序列 Sequence (5′-3′) | 染料 Dye | 退火温度 Tm (℃) | 参考文献 Reference | |
---|---|---|---|---|---|---|
RJ03 | (TGG)8 | F: | GCAGCACTGGCAGGTGG | FAM | 61 | 文献[ Reference [15] |
R: | GATGAATCAGGATGGCTCC | |||||
RJ04 | (TGG)8 | F: | GTGGGTTGGGCTGGAGTC | HEX | 58 | 文献[ Reference [15] |
R: | GCTAATCCATTCCACGCACC | |||||
RJ12 | (AC)7 | F: | ATC ATT CCG ATT TCT TTC TCA CC | FAM | 56 | 文献[ Reference [15] |
R: | TCA CAC TGA TCC CAA TAG ATC AG | |||||
RJ15 | (TG)7 | F: | ATC GAG CAC GTC CAA ATC G | HEX | 56 | 文献[ Reference [15] |
R: | CAC TGG ACA GAC GAC GGT TG | |||||
RJ18 | (TGT)5 | F: | CTG CCC ATG CTC TTC GTC | ROX | 59 | 文献[ Reference [15] |
R: | GAT GAA GTG GGT GCT GCT G | |||||
RJ20 | (CAG)4 | F: | AGA AGA TCG ACG CAC CCG | ROX | 56 | 文献[ Reference [15] |
R: | CCT CCG ATT GGC TTA GGC | |||||
RJ21 | (GTT)6 | F: | TTC CTG GAT TGA ATT CGT CG | FAM | 55 | 文献[ Reference [15] |
R: | CAG TTC TCA CTC GGA CCC AG | |||||
RJ24 | (GTT)5+9 | F: | TTG CTG AGT AGT TTG CGG TGA G | HEX | 58 | 文献[ Reference [15] |
R: | CTC AAG CCC ATC CTC CAA CC | |||||
RJ27 | (TC)10 | F: | CGTCCCGACTAATCTGGTCC | ROX | 52 | 文献[ Reference [15] |
R: | ATGAGTTAGTTTAGATCAGGTCGAC | |||||
CPS08 | (CAG)14 | F: | GAT AAG AAA CAA GGG ACA GC | FAX | 56 | 文献[ Reference [2] |
R: | CAG TGA ACC CAA TTA CTC AG | |||||
CPS09 | (GTT)9 | F: | CGG GAG AAG ACC TGA GC | ROX | 55 | 文献[ Reference [2] |
R: | AGA AAA CGG AAT GTA ATG TG | |||||
CPS10 | (TAG)8 | F: | TCT ACT GGG CAG ACT GGT C | HEX | 56 | 文献[ Reference [2] |
R: | CGG TTT GTT TTG TCG TTT C | |||||
CPS15 | (ATG)5 | F: | GAT GGG GAA AAG TAA GAA GT | FAM | 56 | 文献[ |
R: | GGT GGG GGA TGT AAG TAT GTA | Reference [2] | ||||
CPS34 | (TTTGG)4 | F: | GTT GGC TAC GAG TGG TCA TC | HEX | 56 | 文献[ |
R: | TAA CAC TAC ACA AAA GGG GTC | Reference [2] | ||||
CPS36 | (CTCTAG)3 | F: | TCC AGG CAG TAA ATC AGA CGC | ROX | 58 | 文献[ |
R: | ATC AGC AGG TGT AGC CCC ATC | Reference [2] |
表3
15对引物的遗传多样性信息"
引物 Primer name | 等位基因 Allele | 均匀度 Evenness | 缺失比率 Missing ratio (%) |
---|---|---|---|
RJ12 | 6 | 0.3695 | 2.72 |
RJ15 | 6 | 0.3383 | 5.63 |
RJ18 | 12 | 0.7109 | 8.89 |
RJ27 | 4 | 0.2719 | 4.72 |
RJ20 | 5 | 0.8056 | 8.71 |
RJ21 | 6 | 0.5285 | 2.36 |
RJ24 | 7 | 0.8571 | 3.63 |
CPS08 | 6 | 0.7591 | 0.36 |
CPS09 | 4 | 0.8648 | 9.62 |
CPS10 | 2 | 0.2997 | 0 |
CPS15 | 7 | 0.8171 | 1.09 |
CPS34 | 2 | 0.5949 | 0 |
CPS36 | 3 | 0.6670 | 0.36 |
RJ03 | 7 | 0.8327 | 5.63 |
RJ04 | 4 | 0.2982 | 6.17 |
平均Mean | 5.4 | 0.6010 | 3.99 |
表4
重采基因型及克隆校正群体的基因型多样性统计"
群体 Population | 样本数 N | 多基因座基因型 MLG | 重采基因型编号及数量 Resampled MLG codes and numbers | 基因型多样性 G | 标准化关联指数rbarD | P值 P value |
---|---|---|---|---|---|---|
文县 Wenxian | 27 | 23 | 61 (2), 427 (3), 429 (2) | 0.852 | 0.0139 | 0.186 |
临洮 Lintao | 132 | 111 | 29 (3), 74 (2), 169 (2), 184 (6), 185 (4), 187 (2), 192 (2), 193 (2), 194 (3), 197 (3), 210 (2), 228 (2) | 0.841 | 0.1163 | 0.001 |
平凉 Pingliang | 156 | 152 | 94 (2), 251 (2), 260 (2), 433 (2) | 0.974 | 0.0286 | 0.001 |
临夏 Linxia | 178 | 164 | 141 (5), 143 (2), 146 (2), 254 (2), 289 (2), 308 (2), 314 (2), 320 (2), 394 (2), 489 (2) | 0.921 | 0.0110 | 0.010 |
互助 Huzhu | 47 | 45 | 358 (2), 358 (2) | 0.957 | 0.0416 | 0.002 |
西宁 Xining | 11 | 10 | 272 (2) | 0.909 | 0.0718 | 0.025 |
总数 Total | 551 | 505 | 32 | 0.917 | 0.0260 | 0.001 |
表5
甘肃和青海两省6个小麦条锈菌群体成对的群体遗传分化系数(对角线下部)和基因流(对角线上部)"
文县Wenxian | 临洮Lintao | 平凉Pingliang | 临夏Linxia | 互助Huzhu | 西宁Xining | |
---|---|---|---|---|---|---|
文县 Wenxian | 2.408 | 2.337 | 3.408 | 2.916 | 1.074 | |
临洮 Lintao | 0.143*** | 3.567 | 3.541 | 4.426 | 1.036 | |
平凉 Pingliang | 0.156*** | 0.098*** | 6.238 | 5.217 | 2.601 | |
临夏 Linxia | 0.114*** | 0.103*** | 0.056*** | 8.055 | 2.072 | |
互助 Huzhu | 0.112*** | 0.084*** | 0.069*** | 0.043*** | 1.373 | |
西宁 Xining | 0.234*** | 0.246*** | 0.124*** | 0.138*** | 0.178*** |
[1] | 李振岐, 曾士迈. 中国小麦锈病, 北京: 中国农业出版社, 2002. |
LI Z Q, ZENG S M. Wheat Rust in China. Beijing: China Agriculture Press, 2002. (in Chinese) | |
[2] |
CHEN C Q, ZHENG W M, BUCHENAUER H, HUANG L L, LU N H, KANG Z S. Isolation of microsatellite loci from expressed sequence tag library of Puccinia striiformis f. sp. tritici. Molecular Ecology Resources, 2009,9:236-238.
doi: 10.1111/j.1755-0998.2008.02423.x pmid: 21564613 |
[3] |
CHEN X M. Epidemiology and control of stripe rust [ Puccinia striiformis f. sp. tritici] on wheat. Canadian Journal of Plant Pathology, 2005,27:314-337.
doi: 10.1080/07060660509507230 |
[4] | 马占鸿. 中国小麦条锈病研究与防控. 植物保护学报, 2018,45(1):1-6. |
MA Z H. Researches and control of wheat stripe rust in China. Journal of Plant Protection, 2018,45(1):1-6. (in Chinese) | |
[5] |
陈万权, 康振生, 马占鸿, 徐世昌, 金社林, 姜玉英. 中国小麦条锈病综合治理理论与实践. 中国农业科学, 2013,46(20):4254-4262.
doi: 10.3864/j.issn.0578-1752.2013.20.008 |
CHEN W Q, KANG Z S, MA Z H, XU S C, JIN S L, JIANG Y Y. Integrated management of wheat stripe rust caused by Puccinia striiformis f. sp. tritici in China. Scientia Agricultura Sinica, 2013,46(20):4254-4262. (in Chinese)
doi: 10.3864/j.issn.0578-1752.2013.20.008 |
|
[6] |
HU X P, MA L J, LIU T G, WANG C H, PENG Y L, PU Q, XU X M. Population genetic analysis of Puccinia striiformis f. sp. tritici suggests two distinct populations in Tibet and the other regions of China. Plant Disease, 2017,101(2):288-296.
doi: 10.1094/PDIS-02-16-0190-RE pmid: 30681929 |
[7] |
LIANG J M, LIU X F, LI Y, WAN Q, MA Z H, LUO Y. Population genetic structure and the migration of Puccinia striiformis f. sp. tritici between the Gansu and Sichuan Basin populations of China. Phytopathology, 2016,106(2):192-201.
doi: 10.1094/PHYTO-03-15-0081-R pmid: 26506459 |
[8] |
LIANG J M, WAN Q, LUO Y, MA Z H. Population genetic structures of Puccinia striiformis in Ningxia and Gansu provinces of China. Plant Disease, 2013,97(4):501-509.
doi: 10.1094/PDIS-01-12-0072-RE |
[9] |
LIU X F, HUANG C, SUN Z Y, LIANG J M, LUO Y, MA Z H. Analysis of population structure of Puccinia striiformis in Yunnan Province of China by using AFLP. European Journal of Plant Pathology, 2011,129:43-55.
doi: 10.1007/s10658-010-9688-8 |
[10] |
LU N H, WANG J, CHEN X M, ZHAN G M, CHEN C Q, HUANG L L, KANG Z S. Spatial genetic diversity and interregional spread of Puccinia striiformis f. sp. tritici in Northwest China. European Journal of Plant Pathology, 2011,131:685-693.
doi: 10.1007/s10658-011-9842-y |
[11] | 陆宁海, 郑文明, 王建锋, 詹刚明, 黄丽丽, 康振生. 陇南地区小麦条锈菌群体遗传多样性SSR分析. 中国农业科学, 2009,42(8):2763-2770. |
LU N H, ZHENG W M, WANG J F, ZHAN G M, HUANG L L, KANG Z S. SSR analysis of population genetic diversity of Puccinia striiformis f. sp. tritici in Longnan Region of Gansu, China. Scientia Agricultura Sinica, 2009,42(8):2763-2770. (in Chinese) | |
[12] | 张羽. 甘肃、青海、新疆、西藏四省小麦条锈菌毒性及遗传多样性分析[D]. 雅安: 四川农业大学, 2013. |
ZHANG Y. Virulence and genetic polymorphism of Puccinia striiformis f. sp. tritici in Gansu, Qinghai, Xinjiang and Tibet provinces[D]. Yaan: Sichuan Agricultural University, 2013. (in Chinese) | |
[13] | 张勃, 黄瑾, 贾秋珍, 曹世勤, 孙振宇, 金社林. 甘肃中部及周边地区小麦条锈菌种群的遗传结构分析. 植物保护学报, 2015,42(3):334-339. |
ZHANG B, HUANG J, JIA Q Z, CAO S Q, SUN Z Y, JIN S L. Analysis of population genetic structure of Puccinia striiformis f. sp. tritici from central Gansu and the surrounding areas. Journal of Plant Protection, 2015,42(3):334-339. (in Chinese) | |
[14] | 陈万权, 刘太国, 陈巨莲, 徐世昌. 小麦抗病虫性评价技术规范. 第1 部分: 小麦抗条锈病评价技术规范: NY/T1443.1-2007. 2007-12-01)[2020-01-20]. |
CHEN W Q, LIU T G, CHEN J L, XU S C. Rules for resistance evaluation of wheat to diseases and insect pests. Part 1: Rule for resistance evaluation of wheat to yellow rust (Puccinia striiformis West. f. sp. tritici Eriks.et Henn.): NY/T1443.1-2007. 2007-12-01) [2020-01-20]. (in Chinese) | |
[15] | ENJALBERT J, DUAN X, GIRAUD T, VAUTRIN D, DE VALLAVIEILLE- POPE C, SOLIGNAC M. Isolation of twelve microsatellite loci, using an enrichment protocol, in the phytopathogenic fungus Puccinia striiformis f. sp. tritici. Molecular Ecology Notes, 2002,2:563-565. |
[16] |
PEAKALL R, SMOUSE P E. GenAlEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research—An update. Bioinformatics, 2012,28(19):2537-2539.
doi: 10.1093/bioinformatics/bts460 |
[17] |
KAMVAR Z N, BROOKS J C, GRüNWALD N J. Novel R tools for analysis of genome-wide population genetic data with emphasis on clonality. Frontiers in Genetics, 2015, 6: Article 208.
doi: 10.3389/fgene.2015.00348 pmid: 26734060 |
[18] |
BROWN A H D, FELDMAN M W, NEVO E. Multilocus structure of natural populations of Hordeum spontaneum. Genetics, 1980,96:523-536.
pmid: 17249067 |
[19] |
GRÜNWALD N J, EVERHART S E, KNAUS B J, KAMVAR Z N. Best practices for population genetic analyses. Phytopathology, 2017,107(9):1000-1010.
doi: 10.1094/PHYTO-12-16-0425-RVW pmid: 28513284 |
[20] |
BRUVO R, MICHIELS N K, D’SOUZA T G, SCHULENBURG H. A simple method for the calculation of microsatellite genotype distances irrespective of ploidy level. Molecular Ecology, 2004,13:2101-2106.
doi: 10.1111/j.1365-294X.2004.02209.x pmid: 15189230 |
[21] |
JOMBART T, DEVILLARD S, BALLOUX F. Discriminant analysis of principal components: A new method for the analysis of genetically structured populations. BMC Genetics, 2010,11:94.
doi: 10.1186/1471-2156-11-94 pmid: 20950446 |
[22] | 陈长卿. 中国小麦条锈菌分子群体遗传结构研究[D]. 杨凌: 西北农林科技大学, 2008. |
CHEN C Q. Molecular population genetic structure of Puccinia striiformis f. sp. tritici in China[D]. Yangling: Northwest A&F University, 2008. (in Chinese) | |
[23] | 周祥椿, 杜久元, 尚勋武. 甘肃省小麦品种的现状及对今后育种工作的思考. 甘肃农业科技, 2000(2):3-7. |
ZHOU X C, DU J Y, SHANG X W. The present situation of wheat varieties and the thinking of breeding in future in Gansu Province. Gansu Agricultural Science and Technology, 2000(2):3-7. (in Chinese) | |
[24] |
周祥椿, 杜久元. 陇南小麦生产品种抗条锈病持久性研究. 麦类作物学报, 2006,26(1):108-112.
doi: 10.7606/j.issn.1009-1041.2006.01.028 |
ZHOU X C, DU J Y. Study on resistance durability of commercial wheat cultivars grown in southern region of Gansu Province to stripe rust. Journal of Triticeae Crops, 2006,26(1):108-112. (in Chinese)
doi: 10.7606/j.issn.1009-1041.2006.01.028 |
|
[25] |
DUAN X, TELLIER A, WAN A, LECONTE M, VALLAVIEILLE-POPE C, ENJALBERT J. Puccinia striiformis f. sp. tritici presents high diversity and recombination in the over-summering zone of Gansu, China. Mycologia, 2010,102(1):44-53.
doi: 10.3852/08-098 pmid: 20120228 |
[26] |
MBOUP M, LECONTE M, GAUTIER A, WAN A M, CHEN W, DE VALLAVIEILLE-POPE C, ENJALBERT J. Evidence of genetic recombination in wheat yellow rust populations of a Chinese oversummering area. Fungal Genetics and Biology, 2009,46:299-307.
doi: 10.1016/j.fgb.2008.12.007 pmid: 19570502 |
[27] |
MA L J, KONG X Y, QIAO J X, AN F, HU X P, XU X M. Overwintering of Puccinia striiformis f. sp. tritici on winter wheat at varying altitudes in Gansu and Qinghai provinces. Plant Disease, 2016,100(6):1138-1145.
doi: 10.1094/PDIS-09-15-1112-RE pmid: 30682289 |
[28] | HOVMøLLER M S, JUSTESEN A F, BROWN J K M. Clonality and long-distance migration of Puccinia striiformis f. sp. tritici in north-west Europe. Plant Pathology, 2002,51:24-32. |
[29] |
MCDONALD B A, LINDE C. The population genetics of plant pathogens and breeding strategies for durable resistance. Euphytica, 2002,124:163-180.
doi: 10.1023/A:1015678432355 |
[30] |
ZHAN G M, ZHUANG H, WANG F P, WEI G R, HUANG L L, KANG Z S. Population genetic diversity of Puccinia striiformis f. sp. tritici on different wheat varieties in Tianshui, Gansu Province. World Journal of Microbiology and Biotechnology, 2013,29:173-181.
doi: 10.1007/s11274-012-1170-7 pmid: 23054697 |
[31] |
ZHAO J, WANG L, WANG Z Y, CHEN X M, ZHANG H C, YAO J N, ZHAN G M, CHEN W, HUANG L L, KANG Z S. Identification of eighteen Berberis species as alternate hosts of Puccinia striiformis f. sp. tritici and virulence variation in the pathogen isolates from natural infection of barberry plants in China. Phytopathology, 2013,103(9):927-934.
doi: 10.1094/PHYTO-09-12-0249-R pmid: 23514262 |
[32] |
JIN Y, SZABO L J, CARSON M. Century-old mystery of Puccinia striiformis life history solved with the identification of Berberis as an alternate host. Phytopathology, 2010,100(5):432-435.
doi: 10.1094/PHYTO-100-5-0432 pmid: 20373963 |
[33] | 姚强. 青海省小麦条锈病流行规律研究[D]. 杨凌: 西北农林科技大学, 2018. |
YAO Q. Study on the epidemic rule of wheat stripe rust in Qinghai Province[D]. Yangling: Northwest A&F University, 2018. (in Chinese) | |
[34] | 杜志敏, 姚强, 黄淑杰, 闫佳会, 候璐, 郭青云, 赵杰, 康振生. 青海东部小麦条锈菌转主寄主小檗资源调查与鉴定. 植物病理学报, 2019,43(3):370-378. |
DU Z M, YAO Q, HUANG S J, YAN J H, HOU L, GUO Q Y, ZHAO J, KANG Z S. Investigation and identification of barberry as alternate hosts for Puccinia striiformis f. sp. tritici in eastern Qinghai. Acta Phytopathologica Sinica, 2019,43(3):370-378. (in Chinese) | |
[35] | 赵杰, 郑丹, 左淑霞, 王龙, 黄丽丽, 康振生. 小麦条锈菌有性生殖与毒性变异的研究进展. 植物保护学报, 2018,45(1):7-19. |
ZHAO J, ZHENG D, ZUO S X, WANG L, HUANG L L, KANG Z S. Research advances in alternate host and sexual reproduction of wheat yellow rust pathogen Puccinia striiformis f. sp. tritici Erikss. et Henn. Journal of Plant Protection, 2018,45(1):7-19. (in Chinese) |
[1] | 胡朝月, 王凤涛, 郎晓威, 冯晶, 李俊凯, 蔺瑞明, 姚小波. 小麦抗条锈病基因对中国条锈菌主要流行小种的抗性分析[J]. 中国农业科学, 2022, 55(3): 491-502. |
[2] | 孙小芳,刘敏,潘婷敏,龚国淑. 四川玉米小斑病菌交配型组成与育性分析[J]. 中国农业科学, 2021, 54(12): 2547-2558. |
[3] | 周天宇,李姜玲,杨澜,阮仁武,杨宇衡,李中安. 基于亲本对条锈病敏感性预测小麦杂交种的抗性[J]. 中国农业科学, 2020, 53(9): 1806-1819. |
[4] | 徐默然,蔺瑞明,王凤涛,冯晶,徐世昌. 103份小麦品种(系)抗条锈性和遗传多样性评价及基因检测[J]. 中国农业科学, 2020, 53(4): 748-760. |
[5] | 管方念,龙黎,姚方杰,王昱琦,江千涛,康厚扬,蒋云峰,李伟,邓梅,李豪,陈国跃. 152份黄淮海麦区小麦农家品种抗条锈性评价及重要条锈病抗性基因的分子检测[J]. 中国农业科学, 2020, 53(18): 3629-3637. |
[6] | 魏潇,章秋平,刘宁,张玉萍,徐铭,刘硕,张玉君,马小雪,刘威生. 不同来源中国李(Prunus salicina L.)的多样性与近缘种关系[J]. 中国农业科学, 2019, 52(3): 568-578. |
[7] | 李北,徐琪,杨宇衡,王琪琳,曾庆东,吴建辉,穆京妹,黄丽丽,康振生,韩德俊. 重庆麦区小麦品种(系)抗条锈性评价与基因分析[J]. 中国农业科学, 2017, 50(3): 413-425. |
[8] | 黄亮,刘太国,肖星芷,屈春艳,刘博,高利,罗培高,陈万权. 中国79个小麦品种(系)抗条锈病评价及基因分子检测[J]. 中国农业科学, 2017, 50(16): 3122-3134. |
[9] | 周新力,詹刚明,黄丽丽,韩德俊,康振生. 80份国外春小麦种质资源抗条锈性评价[J]. 中国农业科学, 2015, 48(8): 1518-1526. |
[10] | 王吐虹,郭青云,蔺瑞明,姚强,冯晶,王凤涛,陈万权,徐世昌. 中国40个小麦农家品种和甘肃南部40个生产品种抗条锈病基因推导[J]. 中国农业科学, 2015, 48(19): 3834-3847. |
[11] | 康振生,王晓杰,赵杰,汤春蕾,黄丽丽. 小麦条锈菌致病性及其变异研究进展[J]. 中国农业科学, 2015, 48(17): 3439-3453. |
[12] | 何付新,张阳,秦娟,马微,康振生,郭军. 小麦条锈菌III型磷脂酰肌醇4-羟基激酶基因(PsPik1)的功能分析[J]. 中国农业科学, 2015, 48(16): 3156-3165. |
[13] | 王玲,左示敏,张亚芳,陈宗祥,黄世文,潘学彪. 中国南方八省(自治区)水稻纹枯病菌群体遗传结构的SSR分析[J]. 中国农业科学, 2015, 48(13): 2538-2548. |
[14] | 王文斌1, 2, 张荣胜2, 罗楚平2, 尹小乐2, 刘永锋2, 陆凡1, 2, 陈志谊1, 2. 中国主要稻区稻曲病菌的生物学特性及群体遗传多样性[J]. 中国农业科学, 2014, 47(14): 2762-2773. |
[15] | 陈万权1, 康振生2, 马占鸿3, 徐世昌1, 金社林4, 姜玉英5. 中国小麦条锈病综合治理理论与实践[J]. 中国农业科学, 2013, 46(20): 4254-4262. |
|