中国农业科学 ›› 2017, Vol. 50 ›› Issue (8): 1370-1377.doi: 10.3864/j.issn.0578-1752.2017.08.002

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦TaLox-B等位变异对脂肪氧化酶活性和面粉色泽的影响

张福彦1,陈锋2,程仲杰1,杨保安1,范家霖1,陈晓杰1,张建伟1,陈云堂1,崔龙1

 
  

  1. 1河南省科学院同位素研究所有限责任公司/河南省核农学重点实验室,郑州 450015;2河南农业大学农学院/河南省粮食作物协同创新中心,郑州 450002
  • 收稿日期:2016-11-08 出版日期:2017-04-16 发布日期:2017-04-16
  • 通讯作者: 张建伟,E-mail:zjw10308@163.com。陈锋,E-mail:chf0088@163.com
  • 作者简介:张福彦,E-mail:zhangfuyan704@163.com。
  • 基金资助:
    河南省基础与前沿技术研究计划(162300410169)、河南省科技攻关计划(172102110069)、河南省小麦产业技术体系建设专项资金(Z2010-01-04)

Effects of TaLox-B Alleles on Lipoxygenase Activity and Flour Color in Wheats

ZHANG FuYan1, CHEN Feng2, CHENG ZhongJie1, YANG BaoAn1, FAN JiaLin1, CHEN XiaoJie1ZHANG JianWei1, CHEN YunTang1, CUI Long1   

  1. 1 Isotope Institute Co.,Ltd, Henan Academy of Sciences/Henan Key Laboratory of Nuclear Agricultural Sciences, Zhengzhou  450015; 2 Agronomy College, Henan Agricultural University/Collaborative Innovation Center of Food Crops in Henan     Province, Zhengzhou 450002
  • Received:2016-11-08 Online:2017-04-16 Published:2017-04-16

摘要: 【目的】小麦籽粒脂肪氧化酶(Lox)与面粉色泽密切相关,研究TaLox-B位点上的不同等位变异类型对Lox活性和面粉色泽的影响,为面粉色泽的改良和相关的品质育种工作提供参考依据。【方法】选用122份河南小麦品种(系)为试验材料,用紫外分光光度计和色差仪分别测定其Lox活性和面粉色泽,并利用控制脂肪氧化酶活性位于4BS上的TaLox-B1、TaLox-B2和TaLox-B3位点上的功能标记Lox16、Lox18和Lox-B23对参试材料的Lox基因型进行鉴定。【结果】不同品种间Lox活性及其色泽性状差异达到极显著水平。基因型鉴定结果表明,参试材料的TaLox-B1位点存在TaLox-B1a和TaLox-B1b两种等位变异,所占比例分别为63.9%和39.1%,TaLox-B2位点存在TaLox-B2a和TaLox-B2b两种等位变异,所占比例分别为57.4%和42.6%,TaLox-B3位点也存在TaLox-B3a和TaLox-B3b两种等位变异,所占比例分别为41.8%和48.2%。分析其基因型组合发现,参试材料中共有6种基因型组合类型,依次为TaLox-B1a/TaLox-B2a/TaLox-B3a、TaLox-B1a/TaLox-B2a/TaLox-B3b、TaLox-B1a/TaLox-B2b/ TaLox-B3b、TaLox-B1b/TaLox-B2a/TaLox-B3a、TaLox-B1b/TaLox-B2a/TaLox-B3b和TaLox-B1b/TaLox-B2b/ TaLox-B3b,所占比例分别为41.8%、15.6%、6.6%、28.7%、5.7%和1.6%。分析不同TaLox-B位点基因与Lox活性及红度(a*值)、黄度(b*值)、亮度(L*值)、白度(Wht值)等面粉色泽性状的关系表明,单基因等位变异对Lox活性和面粉色泽的影响不同,3个基因等位变异a*值差异均不显著,对Lox活性的效应,TaLox-B2a高于TaLox-B2b(P<0.05),TaLox-B3a高于TaLox-B3b(P<0.01),TaLox-B2a基因型的Wht值低于TaLox-B2b基因型(P<0.01),TaLox-B3a基因型的Wht值也低于TaLox-B3b基因型(P<0.05)。说明TaLox-B2和TaLox-B3对Lox活性和面粉色泽具有重要影响。进一步分析发现,拥有TaLox-B1a/TaLox-B2a/TaLox-B3a基因型组合的小麦品种(系)的Lox活性和b*值最高,a*值和Wht值最低,而TaLox-B1b/TaLox-B2b/TaLox-B3b基因型组合的小麦品种(系)的L*值、a*值和Wht值最高,其Lox活性和b*值最低。【结论】河南小麦中所发现的6种不同Lox基因型组合中,拥有TaLox-B1a/TaLox-B2a/TaLox-B3a基因型组合的小麦品种(系)的Lox活性相对较高,Wht值相对较低(P<0.05),而拥有TaLox-B1b/TaLox-B2b/TaLox-B3b基因型组合的小麦品种(系)的Lox活性相对较低,Wht值相对较高(P<0.05)。Lox活性的遗传控制在面粉色泽品质改良进程中发挥着关键作用。

关键词: 普通小麦, 脂肪氧化酶基因, 功能标记, 面粉色泽, 河南

Abstract: 【Objective】Lipoxygenase (Lox) is closely related to flour color. The objective of this study was to investigate the influence of different allelic variations at TaLox-B loci on Lox activity and flour color in wheat to provide a theoretical basis for improving flour color and related quality traits in breeding programs.【Method】A total of 122 wheat cultivars and advanced lines from Henan province were used to measure the Lox activities and flour color by spectrophotometer and colorimeter. The functional markers Lox16, Lox18 and Lox-B23 were used to identify the polymorphism of the Lox genes on chromosome 4BS in current Henan wheat cultivars and advanced lines.【Result】There were significant differences of Lox activities, L*, a* and b*values as well as Wht data among different cultivars. Genotyping results indicated two alleles TaLox-B1a and TaLox-B1b at TaLox-B1 locus in these wheat varieties, with 63.9% and 39.1%, respectively. At TaLox-B2 locus, two alleles TaLox-B2a and TaLox-B2b were identified by the functional marker Lox-B23, with 57.4% and 42.6%, respectively. It was also found two alleles TaLox-B3a and TaLox-B3b at the TaLox-B3 locus, with 41.8% and 48.2%, respectively. Six allele combinations of Lox genes, i.e., TaLox-B1a/TaLox-B2a/TaLox-B3a, TaLox-B1a/TaLox-B2a/TaLox-B3b, TaLox-B1a/TaLox-B2b/TaLox-B3b, TaLox-B1b/TaLox-B2a/TaLox-B3a, TaLox-B1b/TaLox-B2a/TaLox-B3b and TaLox-B1b/TaLox-B2b/TaLox-B3b were found with 41.8%, 15.6%, 6.6%, 28.7%, 5.7% and 1.6%, respectively. Analysis of association of Lox alleles at different TaLox-B loci and Lox activities and flour color parameters indicated that the allelic variation at a single locus showed diverse effects on Lox activities and flour color. For a* value, the allelic effect was not significant. For Lox activities, the order of effect was TaLox-B2a > TaLox-B2b (P<0.05), TaLox-B3a > TaLox-B3b (P<0.01). The Wht value of TaLox-B2a was significantly lower than that of TaLox-B2b (P<0.01), and TaLox-B3a showed also a significant lower Wht value than TaLox-B3b (P<0.05). These results suggest that TaLox-B2 and TaLox-B3 significantly affect Lox activities and flour color. Further analysis found that the genotype TaLox-B1a/TaLox-B2a/TaLox-B3a had the highest Lox activity and b* and the lowest a* and Wht value, and TaLox-B1b/TaLox-B2b/TaLox-B3b had the lowest Lox activity and b* and the highest L*, a* and b* values. 【Conclusion】Among the 6 different combinations of three Lox genes in Henan wheat varieties, the genotype TaLox-B1a/TaLox-B2a/TaLox-B3a possess the highest Lox activity and the lowest Wht value (P<0.05), and wheat cultivars with TaLox-B1b/TaLox-B2b/TaLox-B3b have the lowest Lox activity and the highest Wht value (P<0.05). Therefore, the genetic control of Lox activities plays a key role in the improvement of wheat quality.

Key words: common wheat, lipoxygenase gene, functional marker, flour color, Henan