FSHR基因第10外显子多态性及其与小梅山猪产仔数的相关性

doi:10.3864/j.issn.0578-1752.2012.13.017

摘要/Abstract

摘要： 【目的】研究猪FSHR基因第10外显子的多态性及其与小梅山母猪产仔数的相关性。【方法】采用 PCR-SSCP和直接测序方法检测基因多态性，利用最小二乘法分析其对母猪产仔数的关系，同时对多态位点的方差组分进行分析，并预测选择反应。【结果】在小梅山猪、枫泾猪和大白猪这3个群体中，引物P1和P3上分别检测到1个多态位点，其中P1位点可导致氨基酸的改变；引物P6仅在大白猪中检测到1个多态位点；对小梅山猪而言，P1位点的A等位基因和P3位点的D等位基因为优势基因；在P1位点上，两胎以上小梅山母猪中，AA型个体的总产仔数（total number born，TNB）和产活仔数（number born alive，NBA）比BB型分别高出1.95和1.66头（P＜0.01）；在P3位点上，在两胎以上及所有胎次的母猪中，DD型个体的TNB和NBA均极显著高于CC型（P＜0.01）；小梅山母猪的TNB和NBA在P1和P3位点上的遗传主要受到加性效应的影响。【结论】FSHR基因第10外显子P1和P3位点的A和D等位基因对小梅山猪产仔数有显著影响。

关键词: FSHR基因, 多态性, 小梅山猪, PCR-SSCP

Abstract: 【Objective】The effect of polymorphism of exon10 of FSHR gene on litter size in Xiaomeishan pigs was studied.【Method】The polymorphism of exon10 of FSHR gene was investigated by PCR-SSCP and direct sequencing methods, and the effect of exon10 of FSHR gene on litter size traits in Xiaomeishan pigs was analyzed using the least square analysis. Simultaneously the variance components were analyzed and selective reaction was predicted.【Result】 There was one novel polymorphic locus dected by primers P1 and P3, respectively, and the former made amino acid change, among Xiaomeishan, Fengjing and Large White pigs. One polymorphic locus detected by primer P6 was only in Large White pigs. Allele A at locus P1 and allele D at P3 locus were the dominant alleles in Xiaomeishan pigs. AA genotypic sow of Xiaomeishan pigs produced 1.95 TNB (total number born) and 1.66 NBA (number born alive) more than BB genotypic sow at locus P1 after the second parity, respectively (P＜0.01). And DD genotypic sow of Xiaomeishan pigs produced more TNB and NBA than CC genotypic sow at locus P3 after the second parity and in all parities, respectively (P＜0.01). The heredity of TNB and NBA in Xiaomeishan pigs was mostly influenced by the genetic additive effect.【Conclusion】Allele A at locus P1 and allele D at locus P3 in exon10 of FSHR gene had significant effect on litter sizes in Xiaomeishan pigs.

Key words: FSHR gene, polymorphism, Xiaomeishan pigs, PCR-SSCP

吴井生, 王金玉. FSHR基因第10外显子多态性及其与小梅山猪产仔数的相关性[J]. 中国农业科学, 2012, 45(13): 2728-2736.

WU Jing-Sheng, WANG Jin-Yu. Polymorphism of Exon10 of FSHR Gene and Its Relationship with Litter Size in Xiaomeishan Pigs[J]. Scientia Agricultura Sinica, 2012, 45(13): 2728-2736.

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参考文献

[1]张似青, 陆林根, 张江, 秦德兴, 郁金观. 梅山猪繁殖性状遗传参数及其影响因子分析. 养猪, 2007, 1: 52-54.

Zhang S Q, Lu L G, Zhang J, Qin D X, Yu J G. Analysis of genetic parameters of reproductive traits and its influence factor in Meishan swine. Swine Production, 2007, 1: 52-54. (in Chinese)

[2]贾存灵, 李宁, 魏泽辉, 朱晓萍, 刘海英, 贾志海. 不同BMPRIB基因型小尾寒羊同期发情处理后FSHR和LHR表达量的研究. 中国农业科学, 2005, 39(1): 170-175.

Jia C L, Li N, Wei Z H, Zhu X P, Liu H Y, Jia Z H. Study on FSHR and LHR gene mRNA levels of different BMPIB genotypes from Small Tail Han sheep during the oestrum. Scientia Agricultura Sinica, 2005, 39(1): 170-175. (in Chinese)

[3]刘顺德. 基因标记定位在猪数量性状改良中的应用. 养猪, 1999(2): 28-30.

Liu S D. The utilization of gene mapping in improvement of porcine quantitative trait. Swine Production, 1999(2): 28-30. (in Chinese)

[4]Rannikki A S, Zhang F P, Huhtaniemi I T. Ontogeny of follicle-stimulating hormone receptor gene expression in the rat testis and ovary. Molecular and Cellular Endocrinology, 1995, 107(2): 199-208.

[5]Marsters P, Kendall N R, Campbell B K. Temporal relationships between FSH receptor, type 1 insulin-like growth factor receptor, and aromatase expression during FSH-induced differentiation of bovine granulosa cells maintained in serum-free culture. Molecular and Cellular Endocrinology, 2003, 203(1/2): 117-127.

[6]Daelemans C, Smits G, de Maertelaer V, Costagliola S, Englert Y, Vassart G, Delbaere A. Prediction of severity of symptoms in iatrogenic ovarian hyperstimulation syndrome by follicle-stimulating hormone receptor Ser680Asn polymorphism. Journal of Clinical Endocrinology and Metabolism, 2004, 89(12): 6310-6315.

[7]周立花, 李汶, 卢光琇. FSHR、LHR基因突变与多态对女性生殖的影响. 现代生物医学进展, 2010, 10(1): 186-189.

Zhou L H, Li W, Lu G X. The influence of gene mutations and polymorphism of FSHR and LHR to the female reproduction. Progress in Modern Biomedicine, 2010, 10(1): 186-189. (in Chinese)

[8]Greb R R, Grieshaber K, Gromoll J, Sonntag B, Nieschlag E, Kiesel L, Simoni M. A common single nucleotide polymorphism in exon10 of the human follicle stimulating hormone receptor is a major determinant of length and hormonal dynamics of the menstrual cycle. Journal of Clinical Endocrinology and Metabolism, 2005, 90(8): 4866-4872.

[9]Achrekar S K, Modi D N, Meherji P K, Patel Z M, Mahal S D. Follicle stimulating hormone receptor gene variants in women with primary and secondary amenorrhea. Journal of Assisted Reproduction and Genetics, 2010, 27(6): 317-326.

[10]Rodini G P, Genro V K, Matte U, Pereira F S, Bilibio J P, Greggianin C, Souza C A B, Cunha-Filho J S. There is no complete linkage between the polymorphisms N680S and T307A of the follicle stimulating hormone receptor gene in fertile women. Journal of Assisted Reproduction and Genetics, 2011, 28(3): 221-224.

[11]Rahal P, Latronico A C, Kohek M B, de Lucia R F, Milazzotto M P, Wheeler M B, Ferraz J B, Eler J P, Garcia J F. Polymorphisms in the bovine follicle-stimulating hormone receptor gene. Animal Genetics, 2000, 31(4): 280-281.

[12]魏伍川, 许尚忠. 牛FSHR基因5′端侧翼序列的分析和多态性研究. 畜牧兽医学报, 2002, 33(5): 417-423.

Wei W C, Xu S Z. Characterization and polymorphism of the 5′-flanking region of bovine follicle-stimulating hormone receptor gene. Acta Veterinaria et Zootechnica Sinica, 2002, 33(5): 417-423. (in Chinese)

[13]雷雪芹, 陈宏, 袁志发, 徐廷生, 雷初朝, 孙维斌. 牛FSHR基因第10外显子单核苷酸多态性及其与双胎性状的关系. 中国生物化学与分子生物学报, 2004, 20(1): 34-37.

Lei X Q, Chen H, Yuan Z F, Xu T S, Lei C C, Sun W B. Single nucleotide polymorphism in cow follicle-stimulating hormone receptor gene and its association with bovine twinning traits. Chinese Journal of Biochemistry and Molecular Biology, 2004, 20(1): 34-37. (in Chinese)

[14]黄丹丽, 杨章平, 任湘莲, 毛永江, 郭小雅, 瞿冬艳, 李云龙. 乌珠穆沁羊FSHR基因第10外显子的PCR-SSCP检测及序列分析. 家畜生态学报, 2008, 29(2): 17-20.

Huang D L, Yang Z P, Ren X L, Mao Y J, Guo X Y, Qu D Y, Li Y L. PCR-SSCP detection and DNA sequence analysis of exon10 of FSHR gene in three sheep populations. Acta Ecologiae Animalis Domastici, 2008, 29(2): 17-20. (in Chinese)

[15]蓝贤勇, 陈宏, 潘传英, 雷初朝, 张永德, 于娇. 山羊FSHR基因第10 外显子的PCR-SSCP检测及其序列分析. 农业生物技术学报, 2006, 14(4): 484-488.

Lan X Y, Chen H, Pan C Y, Lei C C, Zhang Y D, Yu J. PCR-SSCP detection and DNA sequence analysis of exon10 of goat follicle-stimulating hormone receptor (FSHR) gene. Journal of Agricultural Biotechnology, 2006, 14(4): 484-488. (in Chinese)

[16]姜怀志. 双羔型辽宁绒山羊FSHR基因SNP分析的研究. 吉林农业大学学报, 2004, 26(5): 550-553.

Jiang H Z. Study of the SNP analysis on FSHR gene of biparous Liaoning Cashmere goat line. Journal of Jilin Agriculture University, 2004, 26(5): 550-553. (in Chinese)

[17]谭广梅, 李拥军, 张丽. 海门山羊FSHR基因外显子10的PCR-SSCP分析. 中国草食动物, 2008, 4: 12-14.

Tan G M, Li Y J, Zhang L. Study on the polymorphism of follicle-stimulating hormone receptor gene (FSHR) in Haimen goat. China Herbivores, 2008, 4: 12-14. (in Chinese)

[18]黄磊, 宇向东, 王永, 穆晓琨, 徐华伟, 马力, 付锡三, 林世武. 乐至黑山羊FSHR第10外显子的SNP研究. 西南民族大学学报: 自然科学版, 2010, 36(5): 753-756.

Huang L, Yu X D, Wang Y, Mu X K, Xu H W, Ma L, Fu X S, Lin S W. SNP analysis on follicle-stimulating hormone receptor gene (FSHR) in Lezhi Black goats. Journal of Southwest University for Nationalities: Natural Science Edition, 2010, 36(5): 753-756. (in Chinese)

[19]Perez M M. Gromoll J, Behre H M, Gassner C, Nieschlag E, Simoni M. Ovarian response to follicle-stimulating hormone (FSH) stimulation depends on the FSH receptor genotype. Journal of Clinical Endocrinology and Metabolism, 2000, 85(9): 3365-3369.

[20]de Castro F, Ruiz R, Montoro L, Pérez-Hernández D, Sánchez-Casas Padilla E, Real L M, Ruiz A. Role of follicle-stimulating hormone receptor Ser680Asn polymorphism in the efficacy of follicle- stimulating hormone. Fertility and Sterility, 2003, 80(3): 571-576.

[21]Loutradis D, Patsoula E, Minas V, Koussidis G A, Antsaklis A, Michalas S, Makrigiannakis A. FSH receptor gene polymorphisms have a role for different ovarian response to stimulation in patients entering IVF/ICSI-ET programs. Journal of Assisted Reproduction and Genetics, 2006, 23(4): 177-184.

[22]Jun J K, Yoon J S, Ku S Y, Choi Y M, Hwang K R, Park S Y, Lee G H, Kim S H, Kim J G, Moon S Y. Follicle-stimulating hormone receptor gene polymorphisms and ovarian responses to controlled ovarian hyper stimulation for IVF-ET. Journal of Human Genetics, 2006, 51(8): 665-670.

[23]Achrekar S K, Modi D N, Desai S K, Mangoli V S, Mangoli R V, Mahale S D. Follicle-stimulating hormone receptor polymorphism (Thr307Ala) is associated with variable ovarian response and ovarian hyperstimulation syndrome in Indian women. Fertility and Sterility, 2009, 91(2): 432-439.

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