普通核桃遗传多样性的AFLP分析

doi:10.3864/j.issn.0578-1752.2011.07.016

摘要/Abstract

摘要： 【目的】从分子水平上探讨普通核桃品种的遗传多样性和亲缘关系，为更有效地保护和利用这些品种资源提供科学依据。【方法】采用AFLP-银染分子标记技术，对131份核桃品种进行遗传多样性和亲缘关系分析，应用NTSYSpc2.11a分析软件对统计结果进行聚类分析。【结果】选用20对多态性高、分辨力强的EcoRⅠ/MseⅠ引物组合分别对供试材料的基因组DNA进行扩增，共获得1 643条清晰可辨的条带，其中多态性带1 512条，平均每个引物组合可检测出82.15个多态性位点，多态性位点百分率高达92.03%。得到131份材料间的相似系数范围为0.637—0.928。当相似系数为0.76时， UPGMA分析将131份资源分成8个品种群。【结论】普通核桃种质资源具有丰富遗传多样性和复杂的遗传背景。在品种群中，通过聚类分析难以将早实核桃和晚实核桃截然分开。

关键词: 核桃 , AFLP , 遗传多样性 , 亲缘关系

Abstract: 【Objective】The genetic diversity and phylogenetic relationship of walnut cultivars were discussed at the molecular level, thus providing a scientific basis for more effective protection and use of these resources.【Method】The genetic diversity and relationship of 131 Juglans regia L. cultivars were analyzed by AFLP (amplified fragment length polymorphism) -silver staining protocol. The specific bands were counted and analyzed by NTSYSpc2.11a software. 【Result】 AFLP fingerprinting of 131 Juglans regia L. cultivars with twenty pairs of EcoRⅠ/MseⅠ primers revealed a total number of 1 643 unambiguous bands, of which 1 512 ones were polymorphic and 82.15 polymorphic bands were detected by each pair of primer on average. The polymorphism frequency was 92.03%. This result showed the abundant diversities of enzyme digestion sites among Juglans regia L. cultivars. As analyzed by NTSYSpc2.11a, the similarity coefficient of 131 Juglans regia L. cultivars ranged from 0.637 to 0.928. These Juglans regia L. cultivars were divided into eight groups by UPGMA (unweighted pair group method with arithmetic aver age) based on similarity coefficient. The genetic relationship of 131 Juglans regia L. cultivars was analyzed according to the similarity coefficient. 【Conclusion】 There are rich genetic diversity and complex genetic background in walnut germplasms, but it is difficult to distinguish distinctly the precocious walnut and serotinous walnut by cluster analysis.

Key words: Juglans regia L. , AFLP , genetic diversity , relationship

王红霞1;赵书岗2;高仪3;张志华1;玄立春4. 普通核桃遗传多样性的AFLP分析[J]. 中国农业科学, 2011, 44(7): 1434-1442.

WANGHong-xia1;ZHAOShu-gang2;GAOYi3;ZHANGZhi-hua1;XUANLi-chun4. Genetic Diversity of Juglans regia L. Cultivars Revealed by AFLP Analysis[J]. Scientia Agricultura Sinica, 2011, 44(7): 1434-1442.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2011.07.016

https://www.chinaagrisci.com/CN/Y2011/V44/I7/1434

参考文献

[1]郗荣庭, 张毅萍. 中国核桃. 北京：中国林业出版社, 1992.
Xi R T, Zhang Y P. Chinese Walnut. Beijing: China Forestry Press, 1992. (in Chinese)
[2]Fjiellstrom R G, Parfitt D E. RFLP inheritance and linkage in walnut. Theoretical and Applied Genetics, 1994, 89: 665-670.
[3]Nicese F P, Hormaza J I, Mcgranahan G H. Molecular characterization and genetic relatedness among walnut (Juglans regia L.) genotype based on RAPD markers. Euphytica, 1998, 101: 199-206.
[4]Damiel P, Gao F Y, Giovanna A. Intersimple sequence repeat markers for fingerprinting and determining genetic relationships of walnut(Juglans regia L.) cultivars. Journal of the American Society for Horticultural Science, 2002, 127(1): 75-81.
[5]Fornari B, Cannata F, Spada M, Malvolti M E. Allozyme analysis of genetic diversity and differentiation in European and Asiatic walnut (Juglans regia L.) populations. Forest Genetics, 1999, 6(2): 115-127.
[6]Fornari B, Malvolti M E, Taurchini D, Fineschi S, Beritognolo I, Maccaglia E, Cannata F. Isozyme and organellar DNA analysis of genetic diversity in natural/naturalised European and Asiatic walnut (Juglans regia L.) populations. Acta Horticulturae, 2001, (544): 167-178.
[7]王滑, 郝俊民, 王宝庆, 裴东. 中国核桃8个天然居群遗传多样性分析. 林业科学, 2007, 43(7): 120-124.
Wang H, Hao J M, Wang B Q, Pei D. SSR analysis of genetic diversity of eight natural walnut populations in China. Scientia Silvae Sinicae, 2007, 43(7): 120-124. (in Chinese)
[8]Foroni I, Woeste K, Monti L M, Rao R. Identification of ‘Sorrento’ walnut using simple sequence repeats (SSRs). Genetic Resources and Crop Evolution, 2007, 54:1081-1094.
[9]Ciarmiello L F, Piccirillo P, Pontecorvo G, de Luca A, Kafantaris I, Woodrow P. A PCR based SNPs marker for specific characterization of English walnut (Juglans regia L.) cultivars. Molecular Biology Reports, 2010, doi: 10.1007/s11033-010-0223-y.
[10]吴燕民, 裴东, 奚声珂, 李嘉瑞. 运用RAPD对核桃属种间亲缘关系的研究. 园艺学报, 27(1): 17-22.
Wu Y M, Pei D, Xi S K, Li J R. A study on the genetic relationship among species in Juglans L. using RAPD markers. Acta Horticulturae Sinica, 2000, 27(1): 17-22. (in Chinese)
[11]Wang H, Zhao S, Zhang Z, Gao Y, Zhao Y, Fang J, He F. Genetic relationship and diversity of eight Juglans species in China estimated through AFLP analysis. Acta Horticulturae(ISHS), 2010, 861: 143-150.
[12]Vos P, Hogers R, Bleeker M. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research, 1995, 23: 4407-4414.
[13]韩洁, 胡楠, 李玉阁, 尚富德. 菊花品种资源遗传多样性的AFLP分析. 园艺学报, 2007, 34 (4): 1041-1046.
Han J, Hu N, Li Y G, Shang F D. Genetic diversity of chrysanthemum cultivars revealed by AFLP analysis. Acta Horticulturae Sinica, 2007, 34 (4): 1041-1046. (in Chinese)
[14]黄建安, 李家贤, 黄意欢, 罗军武, 龚志华, 刘仲华. 茶树品种资源遗传多样性的AFLP研究. 园艺学报, 2006, 33 (2): 317-322.
Huang J A, Li J X, Huang Y H, Luo J W, Gong Z H, Liu Z H. Genetic diversity of tea [Camellia sinensis(L.)O. Kuntze] cultivars revealed by AFLP analysis. Acta Horticulturae Sinica, 2006, 33 (2): 317-322. (in Chinese)
[15]王涛, 祝军, 李光晨, 周爱琴, 张文. 苹果砧木亲缘关系AFLP分析. 中国农业科学, 2001, 34(3): 256-259.
Wang T, Zhu J, Li G C, Zhou A Q, Zhang W. AFLP analysis of genetic relationships in apple rootstocks. Scientia Agricultura Sinica, 2001, 34(3): 256-259. (in Chinese)
[16]杨朝东, 王健, 张俊卫, 张波, 包满珠. 梅花不同样本间亲缘关系的AFLP初步分析. 中国农业科学, 2005, 38(10): 2084-2089.
Yang C D, Wang J, Zhang J W, Zhang B, Bao M Z. A preliminary analysis of genetic relationships for Prunus mume Sieb. et Zucc. by AFLP. Scientia Agricultura Sinica, 2005, 38(10): 2084-2089. (in Chinese)
[17]Tosto D S, Hopp H E. Suitability of AFLP markers for the study of genomic relationships within the Oxalis tuberosa alliance. Plant Systematics and Evolution, 2000, 223: 201-209.
[18]Beedanagari S R, Dove S K, Wood B W, Conner. J. A first linkage map of pecan cultivars based on RAPD and AFLP markers. Theoretical and Applied Genetics, 2005, 110: 1127-1137.
[19]陈静, 王文江. 适于AFLP分析的核桃幼叶DNA提取方法. 河北农业大学学报, 2004, 27(6): 44-47.
Chen J, Wang W J. Extraction of DNA for AFLP amplification reaction in tender walnut leaves. Journal of Agricultural University of Hebei, 2004, 27(6): 44-47. (in Chinese)
[20]高志红, 章镇, 韩振海, 沈志军. 果梅SSR反应体系的优化. 南京农业大学学报, 2002, 25 (4): 19-22.
Gao Z H, Zhang Z, Han Z H, Shen Z J. Optimization on SSR analysis system of Japanese apricot( Prunus mume Sieb.et Zucc.). Journal of Nanjing Agricultural University, 2002, 25 (4): 19-22. (in Chinese)
[21]宋国立, 张春庆, 贾继增, 王坤波, 崔荣霞. 棉花AFLP银染技术及品种指纹图谱应用初报. 棉花学报, 1999, 11(6): 281-283.
Song G L, Zhang C Q, Jia J Z ,Wang K B, Cui R X. Cotton AFLP analysis with silver-staining and preliminary report of variety fingerprinting based on it. Acat Gossypll Sinica, 1999, 11(6): 281-283. (in Chinese)
[22]王红霞, 张志华, 赵书岗, 赵悦平, 玄立春. 核桃种质资源遗传多样性研究中的AFLP技术优化及引物筛选. 华北农学报, 2008, 23(1): 50-54.
Wang H X, Zhang Z H, Zhao S G, Zhao Y P, Xuan L C. Optimization of AFLP fingerprinting and screening of primer pairs in genetic diversity analysis of walnut germplasm. Acta Agriculturae Boreali-Sinica, 2008, 23(1): 50-54. (in Chinese)
[23]王红霞. 核桃遗传多样性分析及核心种质的构建[D]. 河北保定: 河北农业大学, 2006.
Wang H X. Analysis of genetic diversity and establishment of core collection of walnut varieties with AFLP markers[D]. Baoding, Hebei: Hebei Agricultural University, 2006. (in Chinese)
[24]罗正荣, 李发芳, 蔡礼鸿. 部分中国原产甜柿种质的分子系统学研究. 园艺学报, 1999, 26(5): 297-301.
Luo Z R, Li F F, Cai L H. Molecular systematics of China native nonastringent persimmon based on random amplified polymorphic DNA. Acta Horticulturae Sinica, 1999, 26(5): 297-301. (in Chinese)
[25]庞晓明, 邓秀新, 胡春根. 枳属36份特异种质的AFLP指纹图谱构建与分析. 园艺学报, 2003, 30(4): 394-398.
Pang X M, Deng X X, Hu C G. Construction of AFLP fingerprint of 36 poncirus accessions. Acta Horticulturae Sinica, 2003, 30(4): 394-398. (in Chinese)