关键词: 蚕豆(Vcia faba L.), 种质资源, ISSR标记, 遗传多样性, 遗传相似性

Abstract:

【Objective】 The purpose of this research is to analyze the genetic diversity of global faba bean germplasm, to explore their genetic similarity and population structure, and to provide essential information for germplasm evaluation and effective utilization of faba bean genetic resources. 【Method】 The genetic similarity of 383 faba bean accessions from 35 countries was analyzed by using ISSR marker. 【Result】 Eleven ISSR primers generated 229 unambiguous bands, of which 212 were polymorphic, and the rate of polymorphic bands was 0.93. Gene diversity index (H) and allelic richness (NA) of different geographic groups of germplasm ranged from 0.16 to 0.28 and from 104 to 193, respectively. Chinese spring-seeding area group showed the highest genetic diversity (H = 0.28, NA = 193), and America group showed the lowest (H = 0.16, NA = 104). The spring faba bean germplasm was clearly separated from winter faba bean germplasm of China in UPGMA clustering analysis based on ISSR molecular marker data. Germplasm from China is quite distinct to that from exotic accessions. The accessions from Europe had a closer genetic similarity with that from North Africa. While the germplasm resources from Asia, Europe and Africa are closely related to their geographical distribution.【Conclusion】Accessions from spring-seeding area of China were most diverse. Germplasm from America showed lowest diversity. The results indicated that the genetic similarity and diversity of faba bean germplasm are closely associated with their growth habit, their geographical origin and ecological distribution.
Key words: faba bean (Vcia faba L.); germplasm resourses; ISSR marker; genetic diversity; genetic similarity

Key words: Faba bean(Vcia faba L.), Germplasm resourses, ISSR marker, Genetic diversity, Genetic similarity