关键词: 簇毛麦, 普通小麦, 易位系, 条锈病, 遗传分析, SSR标记

Abstract:

【Objective】 The high stripe rust resistance material, translocation line V9125-2, from Triticum aestivum-Haynaldia villosa was studied. Inheritance, location and SSR molecular mapping of stripe rust resistance genes in V9125-2 are important for providing a basis for breeding high stripe rust resistance materials. 【Method】 To identify and tag new resistance genes to stripe rust in the translocation line V9125-2 from Triticum aestivum-Haynaldia villosa, the yellow rust races(Puccinia striiform f.sp. stritici)CYR29, CYR30, CYR31, CYR32, CYR33, Su11-4 and Su11-11 were selected as innoculum of genetic analysis of resistance to stripe rust in the progeny population of V9125-2×Mingxian169 at seedling stage. Using the F2 population inoculated with CYR29 to test SSR analysis, combined with bulked segregation analysis (BSA), 289 pairs of primers were used. The F3 population were used to test the linked markers. And the homologous rate between the stripe rust resistance genes of V9125-2 and the varieties in Huang-Huai wheat grown region was detected. 【Result】 The results showed that one dominant gene conferred resistance to stripe rust race CYR29. One dominant gene and one recessive gene indenpently conferred to CYR30, CYR32, CYR33 and Su11-11. And two pairs of independent dominant genes conferred to CYR31 and Su11-4, respectively. The F2 population inoculated with CYR29 were used for SSR analysis, temporarily designated as YrWV. SSR markers, as well as 289 pair primers, combined with bulked segregation analysis (BSA) revealed that six SSR markers, Xbarc87, Xwmc463, Xwmc405, Xbarc126, Xwmc438 and Xgwm473, located on chromosome 7DS were linked to YrWV, and their genetic distances to YrWV were 9.1, 3.9, 5.1, 12.6, 29.0 and 57.4cM, respectively, and the six sites linked to YrWV were validated by a segregated ratio of F3 lines. These markers would be used for breeding new wheat cultivars with marker-assisted selection. A very low homologous rate was found in 43 varieties in Huang-Huai wheat grown region tested with YrWV tags. 【Conclusion】 The result realed that the six SSR markers, Xbarc87, Xwmc463, Xwmc405, Xbarc126, Xwmc438 and Xgwm473, located on the short arm of chromosome 7D in wheat were linked to YrWV. It may be a new wheat stripe rust resistant gene which comes from Haynaldia villosa.

Key words: Haynaldia villosa, common wheat (Triticum aestivum), translocation lines, stripe rust, genetic analysis, SSR marker