Cre/loxP系统,位点特异性重组,转基因,载体构建," /> Cre/loxP系统,位点特异性重组,转基因,载体构建,"/> Cre/loxP system,site-specific recombination,transgene,construction of vector
,"/> <font face="Verdana">Cre/loxP位点特异性重组系统在植物中应用的研究进展</font>

中国农业科学 ›› 2010, Vol. 43 ›› Issue (6): 1099-1107 .doi: 10.3864/j.issn.0578-1752.2010.06.001

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

Cre/loxP位点特异性重组系统在植物中应用的研究进展

孙家利,闫晓红,王力军,魏文辉   

  1. (中国农业科学院油料作物研究所基因组学与分子生物学研究室/农业部油料作物生物学重点开放实验室)

  • 收稿日期:2009-09-07 修回日期:2009-11-26 出版日期:2010-03-15 发布日期:2010-03-15
  • 通讯作者: 魏文辉

Progress in the Application Study of Cre/loxP Site-Specific Recombination System in Plants#br#

SUN Jia-li, YAN Xiao-hong, WANG Li-jun, WEI Wen-hui#br#   

  1. (中国农业科学院油料作物研究所基因组学与分子生物学研究室/农业部油料作物生物学重点开放实验室)

  • Received:2009-09-07 Revised:2009-11-26 Online:2010-03-15 Published:2010-03-15
  • Contact: WEI Wen-hui

摘要:

Cre/loxP位点特异性重组系统自发现以来,在植物基因重组领域得到了广泛应用,已成为提高转基因植物安全性的有效方法。本文介绍了该系统的基本概况及其在转基因植物表达载体构建方面的应用,尤其是在基因删除、定点整合、多基因表达以及杂种优势利用等方面的应用做了重点阐述。

关键词: Cre/loxP系统')">Cre/loxP系统, 位点特异性重组, 转基因, 载体构建

Abstract:

The Cre/loxP site-specific recombination system has been widely applied to gene recombination in plants, and it has become a powerful tool to enhance the safety of transgenic plants. The present review describes an overview of the basic profile of Cre/loxP system and its applications in the construction of plant expression vector, especially in gene deletion, site-specific gene integration, multi-gene expression and heterosis application.

Key words: Cre/loxP system')">Cre/loxP system, site-specific recombination, transgene, construction of vector