关键词: 小鼠, 骨髓间充质干细胞, 分离培养, 诱导分化, 神经细胞, 脂肪细胞

Abstract: Objective To establish a system for isolation and culture of bone marrow mesenchymal stem cells (MSCs) from Kun Ming mouse in intro and to identify characteristic of the cells, directional neuronal and adipocytic induction after culture expansion. Methods　MSC were isolated and cultivated from the bone marrow of Kun Ming mice by investigated their adherence-dependent growth characters; The morphology characters of MSCs were observed under inverted phase contrast microscope; Shape characteristics of MSCs were studied by AKP staining, Hoechst33258 staining, White Giemsa staining; Inducing MSCs into neurone-like cells by β-ME, and staining with HE after inducing, then observe their characteristics under inverted phase contrast microscope; differentiation ratio analysis of differentiated neurocytes were detected by immunocyte-fluorescence of NF-200 and NSE; inducing MSCs into adipocytes by horse serum, observing they process of growth in vitro, differentiation ratio analysis of differentiated adipocytes were detected by Oil Red O stain. Result Separated MSCs were fibroblast-like cells, their have several nucleoluses, positive rates of AKP staining were 93.2％±1.5％. MSCs could differentiate into several phenotype characteristic neurocytes after inducing by β-ME, their shape characteristics were similar with primer passage of mouse full-brain cells. Both NF-200 and NSE immunocyte-fluorescence of Neurones after inducing were positive, positive rates of NF-200 and NSE were 85.4%±1.8% and 82.7%±2.1% respectively; MSCs could differentiate into adipocytes in 100 percent after inducing by horse serum, Oil Red O stain was positive. Conclusion Mesenchymal stem cells of Kun Ming mouse can be successfully isolate and cultivate from mouse bone marrow by keeps its adherence-dependent growth characters. They have directional trans-differentiation potentiality to various neurocytes and adipocytes.

Key words: mice, MSCs, separation and culture, induction and differentiation, neurocytes, adipocytes