中国农业科学 ›› 2007, Vol. 40 ›› Issue (8): 1746-1752 .

• 园艺 • 上一篇    下一篇

白菜转录因子BcBHLHogu的克隆及其特征分析

向 珣,曹家树,叶纨芝,王华新   

  1. 浙江大学蔬菜研究所/农业部重点实验室园艺植物生长与发育调控实验室
  • 收稿日期:2006-11-27 修回日期:2007-02-06 出版日期:2007-08-10 发布日期:2007-08-10
  • 通讯作者: 曹家树

Isolation and characterization analysis of a novel bHLH Gene, BcBHLHogu, in Chinese cabbage (Brassica campestris L. ssp. chinensis Makino)

  

  1. 浙江大学蔬菜研究所/农业部重点实验室园艺植物生长与发育调控实验室
  • Received:2006-11-27 Revised:2007-02-06 Online:2007-08-10 Published:2007-08-10

摘要: 【目的】探明白菜‘矮脚黄’OguCMS核质互作的机制。【方法】采用cDNA-AFLP技术分析白菜‘矮脚黄’OguCMS及其保持系花蕾基因的表达差异,获得一个在保持系早期花蕾不表达但在OguCMS早、中、晚花蕾中稳定表达的差异片段。利用RACE技术获得其cDNA全长,命名为BcBHLHogu(GenBank 登录号:EF127860)。【结果】该基因编码122个氨基酸,具有basic helix-loop- helix(bHLH)结构域;进化分析表明,BcBHLHogu与拟南芥AtBHLH060同源性最高,是冗余基因AtBHLHs060/048的直系同源基因,其功能与BEEs1/2/3(3个油菜素内酯信号的早期应答冗余基因)相似。【结论】BcBHLHogu是白菜bHLH转录因子家族的一个新成员,涉及到花器官发育信号事件,其功能与油菜素内酯应答有关。

关键词: 白菜, bHLH转录因子, 克隆, 特征分析

Abstract: 【Objective】This study was carried out in order to better understanding the mechanism of nuclear-cytoplasmic interactions in OguCMS Chinese cabbage pak-choi (Brassica campestris L. ssp. chinensis Makino var. communis Tsen et Lee cv. Aijiaohuang).【Method】Expreesion profiling was performed using cDNA-AFLP technology on floral buds of OguCMS lines and maintainers in Chinese cabbage pak-choi. A differentially expressed cDNA fragment was obtained,which was unexpressed in early-stage buds of maintainers, but expressed steabilely in OguCMS floral buds. The full cDNA, designated as BcBHLogu(GenBank accession numbers:EF127860), was obtained by RACE. 【Results】 BcBHLogu coded 122 amino acids , contained a bHLH domain, and shared high homology with AtBHLH060. The phyogenetic analysis indicted BcBHLogu and AtBHLHs060/048 were orthologs genes. Its function was similar to BEEs1/2/3,which were three redundant Brassinostreriod early response genes.【Conclusion】BcBHLHogu was predicted a novel bHLH transcription factor in Chinese cabbage, which gene appears to be involved in developmental signaling events for floral processes and had a function related to Brassinosteriods signaling response.

Key words: Chinese cabbage (Brassica campestris ssp. Chinensis), bHLH transcription factor, Cloning, Characterization