关键词: 小麦地方品种, 形态学性状和农艺性状, 种子贮藏蛋白, SSR分子标记, 异质性

Abstract: 【Objective】 The objective of the present study was to determine of the genetic variation and genetic differentiation of the wheat landrace Daqingmang, which originated from the northeastern China. 【Method】Fourteen morphological and agronomic traits, seed storage proteins analyses and 84 simple sequence repeat (SSR) markers were used to detect the genetic relationships among five accessions of the landrace Daqingmang that were collected from different sites. 【Result】The Daqingmang accessions displayed similar performance in morphological and agronomic traits. However, high levels of variations were detected in compositions of gliadins and high-molecular-weiht glutenin subunits (HMW-GS), and 57.1% of the total of 84 SSR primers that distribute in different chromosome arms. Moreover, heterogeneity was detected within the accessions of ZM4401, ZM4402, ZM4407 and ZM4421 in gliadin band patterns and HMW-GS. Microsatellite analysis also revealed the unequal contribution to the genetic variations among Daqingmang accessions. 【Conclusion】Our research showed that landrance of Daqingmang was a population with widely genetic diversity. Although no phenotypic variation was observed, extensive variations in the genes loci of seed storage proteins and many SSR primers loci were detected among the 5 Daqingmang accessions. The results have important significance for effective maintenance and application of genetic resources.

Key words: Wheat landraces, Agro-morphology, Seed storage protein, SSR markers