转cry1Ac/sck基因抗虫水稻对稻田主要非靶标害虫的田间影响评价

中国农业科学 ›› 2007, Vol. 40 ›› Issue (6): 1181-1189 .

转cry1Ac/sck基因抗虫水稻对稻田主要非靶标害虫的田间影响评价

刘雨芳,贺玲,汪琼,胡斯琴,刘文海,陈康贵

湖南科技大学生命科学学院及应用生态学研究所

收稿日期:2006-02-27 修回日期:1900-01-01 出版日期:2007-06-10 发布日期:2007-06-10

Effects of insect-resistant cry1Ac/sck transgenic rice on the key non-target pests in paddy fields and evaluating the ecological safety

湖南科技大学生命科学学院及应用生态学研究所

Received:2006-02-27 Revised:1900-01-01 Online:2007-06-10 Published:2007-06-10

摘要/Abstract

摘要： 【目的】研究转基因抗虫水稻对稻田主要非靶标害虫的田间影响。【方法】以转cry1Ac/ sck双基因抗虫水稻株系MSA、MSB、MSA4及其杂交稻21S/MSB、II-32A/MSB与KF6-304为材料，2002年在福建沙县、2003至2004在湖南湘潭县系统地开展了转基因水稻对稻田主要非靶标害虫白背飞虱、褐飞虱、叶蝉及稻瘿蚊的田间影响评价研究。【结果】秧苗期，MSA、MSB、21S/MSB、II-32A/MSB对稻飞虱产卵没有明显影响，稻飞虱在MSA4与KF6-304秧苗上的产卵量显著低于其对照,但所取样本上的产卵量都非常低。水稻移栽后，转基因水稻株系对白背飞虱与褐飞虱种群数量的影响有一定差异,但与它们的对照均无显著差异,整个生长期的数据分析也显示无显著差异。21S/MSB、II-32A/MSB上的叶蝉数量显著或极显著高于其对照，但叶蝉数量很低,单种叶蝉的种群数量则更低,且MSA、MSB、MSA4与KF6-304没有引起稻田叶蝉数量的明显变化。在成熟收割前，MSA、MSB有增加无效分蘖上葱管数的态势，但在水稻生长中期都对稻瘿蚊有高的抗性，能减少葱管的形成，降低标葱率。【结论】转cry1Ac/sck 双基因抗虫水稻MSA、MSB、MSA4及杂交稻21S/MSB、II-32A/MSB与KF6-304不会引起关键非靶标水稻害虫数量的明显上升。导入相同外源基因的不同水稻株系对关键非靶标水稻害虫的影响在时间动态与程度上存在一定的差异，转基因作物生态安全评价需要进行个案分析。

关键词: 转基因水稻, 非靶标害虫, 白背飞虱, 褐飞虱, 叶蝉, 稻瘿蚊

Abstract: 【Objective】To study the effects of resistant –insects transgenic rice on the key non-target pests in paddy fields. 【Method】The effects of three rice lines (MSA, MSB and MSA4) and three hybrid rice lines (21S/MSB, II-32A/MSB and KF6-304) of transgenic rice with a fused double genes of cry1Ac and sck (cry1Ac/sck rice) on the key non-target pests in paddy fields were investigated and the ecological safety of these cry1Ac/sck rice were evaluated in Sha County, Fujian Province in 2002 and in Xiangtan County, Huana Province in 2003, 2004, respectively.【Result】The significant differences in the fecundity of white back planthopper, Sogatella urcifera were not found between cry1Ac/sck rice MSA、MSB、21S/MSB、II-32A/MSB plots and their control, furthermore, both MSA4 and KF6-304 significant decreased the fecundity of S. urcifera during seedling. Whereas all six cry1Ac/sck rice lines sometimes increased or decreased the number of individuals of S. urcifera and brown planthopper, Nilaparvata lugens in the temporal dynamic, in most cases, there were no significant differences between cry1Ac/sck rice plots and their controls, and the situations in total quantity were similar. Both 21S/MSB and II-32A/MSB stimulated the populations of leafhoppers, however, there were no significant differences between other cry1Ac/sck rice plots and their controls and only a lower total quantity of leafhoppers in paddy fields. MSA and MSB showed high resistance to Pachydiplosis oryzae and decreased the number of shallot tracts and rate of rice clusters with shallot tracts at mid-stage of rice growth whereas these two cry1Ac/sck rice plots have the trends to increase the number of shallot tracts in null ramifications of rice before harvesing.【Result】 The results suggest that cry1Ac/sck rice lines, MSA, MSB, MSA4, 21S/MSB, II-32A/MSB and KF6-304 have not significant negative effects on key non-target pests in paddy fields.

Key words: non-target insect pests, Sogatella urcifera, Nilaparvata lugens, leafhopper, Pachydiplosis oryzae, transgenic rice

刘雨芳,贺玲,汪琼,胡斯琴,刘文海,陈康贵. 转cry1Ac/sck基因抗虫水稻对稻田主要非靶标害虫的田间影响评价[J]. 中国农业科学, 2007, 40(6): 1181-1189 .

. Effects of insect-resistant cry1Ac/sck transgenic rice on the key non-target pests in paddy fields and evaluating the ecological safety[J]. Scientia Agricultura Sinica, 2007, 40(6): 1181-1189 .

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/

https://www.chinaagrisci.com/CN/Y2007/V40/I6/1181

[1]	邬伟,徐慧丽,王正亮,俞晓平. 褐飞虱丝氨酸蛋白酶抑制剂基因Nlserpin2的克隆及其功能分析[J]. 中国农业科学, 2022, 55(12): 2338-2346.
[2]	於卫东,潘碧莹,邱玲玉,黄镇,周泰,叶林,唐斌,王世贵. 两个褐飞虱海藻糖转运蛋白基因的结构及调控海藻糖代谢功能[J]. 中国农业科学, 2020, 53(23): 4802-4812.
[3]	张道伟,康奎,余亚娅,匡富萍,潘碧莹,陈静,唐斌. 白背飞虱酚氧化酶原PPO基因特性及其免疫应答[J]. 中国农业科学, 2020, 53(15): 3108-3119.
[4]	赵艳,王天圻,朱军莉. 基于PTN系统分析不同种植地转基因水稻种子可培养内生真菌菌群的多样性[J]. 中国农业科学, 2020, 53(11): 2305-2320.
[5]	丁艳娟,刘永康,罗雨嘉,邓颖梅,徐红星,唐斌,徐彩娣. 褐飞虱GSK-3调控糖原与海藻糖代谢的潜在功能[J]. 中国农业科学, 2019, 52(7): 1237-1246.
[6]	唐斌,沈祺达,曾伯平,肖仲久,邱玲玉,潘碧莹,李昆,张道伟. 褐飞虱一个新的海藻糖合成酶基因的特性、发育表达及RNAi效果分析[J]. 中国农业科学, 2019, 52(3): 466-477.
[7]	张道伟,余亚娅,潘碧莹,康奎,曾伯平,陈静,唐斌. 白背飞虱海藻糖合成酶基因调控几丁质合成的功能[J]. 中国农业科学, 2019, 52(19): 3357-3366.
[8]	贺静澜,张明,刘瑞莹,万贵钧,潘卫东,陈法军. 近零磁场下干扰磁响应关键基因对褐飞虱寿命的影响[J]. 中国农业科学, 2019, 52(1): 45-55.
[9]	张露，朱世城，郑好，沈祺达，王世贵，唐斌. 褐飞虱海藻糖酶基因在表皮几丁质代谢中的调控作用[J]. 中国农业科学, 2017, 50(6): 1047-1056.
[10]	李冠楠，苗昌见，李为争，王高平，刘晓岚，郭线茹，闫凤鸣. 吡虫啉悬浮种衣剂对玉米田节肢动物群落及主要非靶标害虫的影响[J]. 中国农业科学, 2017, 50(24): 4735-4746.
[11]	李干金，徐显浩，张海亮，朱敏，崔旭红. 短时高温暴露对褐飞虱存活和生殖特性的影响[J]. 中国农业科学, 2015, 48(9): 1747-1755.
[12]	赵艳，李燕燕. 利用PTN系统快速解析转基因水稻种子可溶性蛋白非预期变异的来源[J]. 中国农业科学, 2015, 48(22): 4397-4407.
[13]	王博，姚云，徐泽炜，林欣大. 褐飞虱Yellow基因的克隆及功能[J]. 中国农业科学, 2015, 48(15): 2976-2984.
[14]	赵艺泽，刘艳，王锡锋. 利用酵母双杂交系统筛选介体异沙叶蝉中与小麦矮缩病毒外壳蛋白互作的蛋白质[J]. 中国农业科学, 2015, 48(12): 2354-2363.
[15]	李伟, 郭建夫, 袁红旭, 李玥仁, 刘月廉, 黄永相, 蒋世河. 抗真菌转基因水稻对稻田靶标病害和非靶标病虫害的影响[J]. 中国农业科学, 2012, 45(24): 5032-5039.