中国农业科学 ›› 2007, Vol. 40 ›› Issue (2): 291-297 .

• 植物保护 • 上一篇    下一篇

花生品种对疮痂病抗性及其机制的研究

方树民,王正荣,柯玉琴,陈玉森,黄春梅,于建兴   

  1. 福建农林大学植物保护学院
  • 收稿日期:2005-10-20 修回日期:1900-01-01 出版日期:2007-02-10 发布日期:2007-02-10

The evaluation of resistance and study of resistant mechanism of peanut varieties to scab disease

  

  1. 福建农林大学植物保护学院
  • Received:2005-10-20 Revised:1900-01-01 Online:2007-02-10 Published:2007-02-10

摘要: 【目的】研究花生栽培品种对疮痂病的抗性差异,为品种抗性鉴定和利用提供科学依据。【方法】用病菌孢子悬液对32份品种作田间接种鉴定,对不同叶位病情做调查;用不同病级叶片作酶活性和丙二醛含量测定。对8份品种作表皮组织显微计测和病程观察。【结果】粤油5号等11份表现感病;濮花16等10份为中感;淮花8号等8份和徐花8号等3份则为中抗和抗病。抗病型品种(MR-R)顶端嫩叶发病率0~23.8%,Ⅴ、Ⅵ级病叶比率0;感病型品种(MS-S)则分别为84%~100%和10.7%~37.1%。随着叶片病害逐级加重,POD活性和MDA含量相应上升;CAT和SOD活性则呈下降趋势。不同品种皮层结构差异决定对疮痂病抗性,抗病品种具较厚的角质层,叶片气孔较少;且病菌潜育期长,产生病斑少,果荚带菌率低。【结论】主栽泉花10号等感病品种表皮角质层薄,嫩叶气孔多,病菌潜育期短,产生病斑数多,释放孢子量大,这是导致该病流行的首要因素。

关键词: 花生疮痂病, 品种抗性, 酶活性, 皮层组织结构, 潜育期

Abstract: For detecting the resistance of varieties of peanut against leaf scab, the seedlings of 32 varieties were inoculated with the spore of scab pathogen, and the observation on the disease development in the field was systematically carried out. The results showed that 11 varieties, including Eyou 5 and Huaihua 8, were susceptible; 10 varieties, including Puhua 16 and Jinhuasheng 1, were moderate susceptible; 8 varieties, including Eyou 9 and Huaihua 8, were moderate resistant; 3 varieties, including Xuhua 8 and G/845, were resistant. The results of differents parts of leaves from the cirruses of 8 varieties revealed that the incidence of a disease, the top leaf of the MR-R varieties, was 0%-23.8%, the rate of the diseased leaf belonging to the V、VI degrees;while the MS-S varieties ,the incidence of a disease is 84.0%-100% and 10.7%-37.1%.The results of leaf activities of enzyme and the content tests of MDA of different disease levels from different susceptible varieties revealed that activities of POD and the content tests of MDA raised accordingly and had close relation; while the activities of CAT and SOD descended .The results of micro-measure of different parts and phases revealed that the different reactions to scab pathogen were determined by the cortical structural differences of varieties. The resistant varieties and aging structures have thicker cutin, less stoma density in the leaf; The susceptible varieties and tender structures have thinner cutin, much stoma density in the leaf.