中国农业科学 ›› 2007, Vol. 40 ›› Issue (10): 2355-2360 .

• 研究简报 • 上一篇    下一篇

利用基因芯片研究小麦耐盐突变体盐胁迫条件下基因的表达图谱

赵宝存,赵 芊,葛荣朝,沈银柱,黄占景   

  1. 河北师范大学生命科学学院
  • 收稿日期:2006-03-01 修回日期:2006-06-13 出版日期:2007-10-10 发布日期:2007-10-10
  • 通讯作者: 黄占景

Study the expression map of salt-tolerance mutant under salt-stress in wheat using gene microarray

  

  1. 河北师范大学生命科学学院
  • Received:2006-03-01 Revised:2006-06-13 Online:2007-10-10 Published:2007-10-10

摘要: 【目的】研究小麦在不同盐胁迫时间下根部基因的应答反应。【方法】利用基因芯片技术,分析盐胁迫下耐盐小麦RH8706-49的小麦根部基因的表达情况。【结果】获得了61 215个小麦基因的差异表达图谱。在不同盐胁迫时间下大量根部基因的表达发生很大变化,即有盐诱导表达的基因,也有盐抑制表达的基因,同时对杂交数据进行多种聚类分析,并对基因表达差异的原因进行了初步分析。【结论】小麦耐盐机理非常复杂,是大量基因协调表达的结果,其中盐诱导表达基因的作用非常重要。

关键词: 基因芯片, 小麦, 耐盐基因, 盐胁迫, 盐诱导基因

Abstract: 【OBJECTIVE】To study the reaction of different gene of root under salt-tolerance in wheat;【METHOD】Analysed the gene expression of root in wheat using gene chip technology; 【RESULTS】 The expression of all gene in root of wheat salt-tolerance mutant RH8706-49 were analysed using gene chip technology. The responses to different time of salt stress were studied. The differentia expression map of 61,215 genes was gained. The clustering analysis of hybridizing data showed gene expression changed in root under salt stress. Both salt-induced gene and salt-inhabited gene had been found;【CONCLUSION】The mechanism of salt-tolerance in wheat is very complicated. It is the result of a lot of genes expression in line. And the function of the salt-induced gene is very important.

Key words: gene chip, wheat, salt-tolerance gene, salt stress, salt-induced gene