关键词: FAT/CD36, RACE, 实时定量RT-PCR, 黄羽肉鸡, 脂肪

Abstract: Abstract: 【Objective】To clone chicken FAT/CD36 complete cDNA sequence and study the ontogenesis expression pattern of FAT/CD36 mRNA;【Method】Chicken FAT/CD36 complete cDNA was cloned by 5’- and 3’-RACE. Subcutaneous fat and visceral fat samples were collected and weighed from both male and female Yellow-feathered broilers on d22, d29, d42 and d56 (n=10). Fat content of breast muscle and thigh muscle were determined on d29 and d56. Real time RT-PCR was conducted to investigate the tissue specific ontogenesis expression pattern of FAT/CD36 mRNA in breast muscle, thigh muscle, subcutaneous fat and visceral fat samples collected from both male and female Yellow-feathered broilers;【Result】We have cloned chicken FAT/CD36 complete cDNA sequence of 2243bp(GenBank: DQ323177), which including the 1416bp ORF. The accumulation of subcutaneous fat and visceral fat gradually increased associated with age and the FAT/CD36 mRNA expression levels in subcutaneous fat and visceral fat of male broilers also increased gradually, the highest expression level proved to be in the visceral fat. The FAT/CD36 mRNA expression levels of female broilers were relatively high during the development prophase (d22 and d29), whereas a degressive expression pattern was showed during the development anaphase (d42 and d56);【Conclusion】We have successfully cloned chicken FAT/CD36 complete cDNA sequence. The expression pattern of FAT/CD36 mRNA in muscle and fat were different with gender.

Key words: FAT/CD36, RACE, Real-time PCR, Yellow-feathered broilers, Fat