中国农业科学 ›› 2007, Vol. 40 ›› Issue (10): 2289-2295 .

• 园艺 • 上一篇    下一篇

不同部位烟叶海绵与栅栏细胞中主要化学成分研究

黄 勇,周冀衡,刘建利,杨虹琦,王 刚,王 勇,张苧元   

  1. 湖南农业大学生物科学技术学院/烟草科学与工程技术重点实验室
  • 收稿日期:2006-11-13 修回日期:2007-02-07 出版日期:2007-10-10 发布日期:2007-10-10
  • 通讯作者: 周冀衡

Study on Main Chemical Component of Spongy Mesophyll

  

  1. 湖南农业大学生物科学技术学院/烟草科学与工程技术重点实验室
  • Received:2006-11-13 Revised:2007-02-07 Online:2007-10-10 Published:2007-10-10

摘要: 【目的】定量了解不同叶位海绵与栅栏细胞主要品质成分的差异。【方法】采用酶解方法分离‘K326’和红花大金元下部(第4叶)、中部(第10叶)、上部(第16叶)适熟叶片海绵与栅栏细胞,然后通过气相色谱分析鉴定非挥发性有机酸,分光光度计法分析质体色素、糖类及烟碱。【结果】样品海绵与栅栏细胞的数量百分比分别为85%,80%以上;多元有机酸在海绵细胞中含量较高,高级脂肪酸、质体色素、糖类均是栅栏细胞中含量高,而烟碱却是海绵细胞含量高于栅栏细胞;无论是海绵细胞,还是栅栏细胞,上部叶叶绿素含量较高,下部叶非挥发性有机酸含量较高,中部叶类胡萝卜素、总糖和还原糖含量较高,非还原糖与叶位无关。烟碱与含量与叶位正相关。【结论】酶解法能够有效分离叶片细胞,不同基因型、不同叶位、不同细胞都存在较大的差异,中部叶、栅栏细胞有益成分较高。

关键词: 品质成分, 细胞, 叶位, 品种, 烤烟

Abstract: The palisade mesophyll and spongy mesophyll of cutter different riped leaf-position (lower,4th,cutter,10th,upper,16th) of Flue-cured Tobacco(K326 andHongDa) were divided by pectinase, then no-volatility organic acid was detected by gas chromatography, and plastid pigment or glucide or nicotine was analyzed by spectrophotometer. It showed that the content of multiple organic acid and nicotine of spongy mesophyll was higher than that of palisade mesophyll, and other components were on the contrary. No matter the palisade mesophyll or spongy mesophyll,the content of chlorophyllous in upper leaf cells was highest, and the content of no-volatility organic acid in lower leaf cells was highest, and the total content of carotin, glucide,deoxidize sugar in cutter leaf cells was highest, and the content of no-deoxidize sugar in cells hasn’t pertinence among the leaf position. The content of nicotine in cells was positive correlation with leaf position.

Key words: No-volatility organic acid, Plastid pigment, Glucide, Nicotine, Palisade mesophyll, Spongy mesophyll, Leaf position